Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection
Jingjing Zhong, Ming Wei, Chunxia Yang, Yudong Yin, Yu Bai, Ran Li, Li Gu Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Li Gu, Beiji...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-03-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/molecular-epidemiology-of-community-acquired-methicillin-resistant-sta-peer-reviewed-fulltext-article-IDR |
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| author | Zhong J Wei M Yang C Yin Y Bai Y Li R Gu L |
| author_facet | Zhong J Wei M Yang C Yin Y Bai Y Li R Gu L |
| author_sort | Zhong J |
| collection | DOAJ |
| description | Jingjing Zhong, Ming Wei, Chunxia Yang, Yudong Yin, Yu Bai, Ran Li, Li Gu Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Li Gu, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100000, People’s Republic of China, Email guli2013227@foxmail.comPurpose: Since community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized, the molecular epidemiology of CA-MRSA in China has been diverse. It is unclear whether different sites of CA-MRSA infection differ in antimicrobial resistance and clinical characteristics. The purpose of this study was to identify the molecular types, virulence factors and antimicrobial resistance of CA-MRSA strains and to analyze the clinical characteristics of different sites of CA-MRSA infection.Methods: 26 CA-MRSA strains were screened from Beijing Chao-Yang Hospital from 2014 to 2022. SCCmec type, MLST type, spa type, Panton–Valentine leukocidin (PVL), hemolysin α (Hla), phenolic soluble regulatory protein α (PSMα), toxic shock syndrome toxin-1 (TSST-1), and enterotoxin (SE) A to E were detected by PCR and gene sequencing. Antimicrobial susceptibility tests and the clinical features of CA-MRSA infection cases were collected for statistical analysis.Results: The predominant type of CA-MRSA was ST59-t437-IV. New non-epidemic types, SCCmec VII, were also found. PVL was seen in 65.4% of CA-MRSA strains and TSST-1 was only be detected in 3.8% of CA-MRSA strain which caused poor prognosis. There were three types of infections: pneumonia (61.5%), infective endocarditis (7.7%), and skin and soft tissue infections (SSTIs) (30.8%). CA-MRSA pneumonia cases were secondary to influenza infection (37.5%). Patients with CA-MRSA-associated infective endocarditis were more likely to have underlying cardiac diseases. Patients with CA-MRSA-associated SSTIs were more likely to have a history of diabetes mellitus, and strains in this group were more susceptible to erythromycin and clindamycin.Conclusion: ST59-t437-IV was the primary CA-MRSA type in our research and in China. We proposed that TSST-1 might be one of the indicators to predict the severity and prognosis of CA-MRSA infection. Different sites of CA-MRSA infection had difference in antibiotics susceptibility testing and underlying diseases of patients. It could provide a new perspective on treating different types of CA-MRSA infection.Keywords: community, acquired methicillin, resistant Staphylococcus aureus, staphylococcal chromosomal cassette mec type, Panton-Valentine leukocidin, antibiotic resistance, pneumonia |
| first_indexed | 2024-04-10T00:35:21Z |
| format | Article |
| id | doaj.art-82ef6a35ad81410fbac7c7c5f0ca67a9 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-10T00:35:21Z |
| publishDate | 2023-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-82ef6a35ad81410fbac7c7c5f0ca67a92023-03-14T18:21:58ZengDove Medical PressInfection and Drug Resistance1178-69732023-03-01Volume 161485149782293Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of InfectionZhong JWei MYang CYin YBai YLi RGu LJingjing Zhong, Ming Wei, Chunxia Yang, Yudong Yin, Yu Bai, Ran Li, Li Gu Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Li Gu, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100000, People’s Republic of China, Email guli2013227@foxmail.comPurpose: Since community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized, the molecular epidemiology of CA-MRSA in China has been diverse. It is unclear whether different sites of CA-MRSA infection differ in antimicrobial resistance and clinical characteristics. The purpose of this study was to identify the molecular types, virulence factors and antimicrobial resistance of CA-MRSA strains and to analyze the clinical characteristics of different sites of CA-MRSA infection.Methods: 26 CA-MRSA strains were screened from Beijing Chao-Yang Hospital from 2014 to 2022. SCCmec type, MLST type, spa type, Panton–Valentine leukocidin (PVL), hemolysin α (Hla), phenolic soluble regulatory protein α (PSMα), toxic shock syndrome toxin-1 (TSST-1), and enterotoxin (SE) A to E were detected by PCR and gene sequencing. Antimicrobial susceptibility tests and the clinical features of CA-MRSA infection cases were collected for statistical analysis.Results: The predominant type of CA-MRSA was ST59-t437-IV. New non-epidemic types, SCCmec VII, were also found. PVL was seen in 65.4% of CA-MRSA strains and TSST-1 was only be detected in 3.8% of CA-MRSA strain which caused poor prognosis. There were three types of infections: pneumonia (61.5%), infective endocarditis (7.7%), and skin and soft tissue infections (SSTIs) (30.8%). CA-MRSA pneumonia cases were secondary to influenza infection (37.5%). Patients with CA-MRSA-associated infective endocarditis were more likely to have underlying cardiac diseases. Patients with CA-MRSA-associated SSTIs were more likely to have a history of diabetes mellitus, and strains in this group were more susceptible to erythromycin and clindamycin.Conclusion: ST59-t437-IV was the primary CA-MRSA type in our research and in China. We proposed that TSST-1 might be one of the indicators to predict the severity and prognosis of CA-MRSA infection. Different sites of CA-MRSA infection had difference in antibiotics susceptibility testing and underlying diseases of patients. It could provide a new perspective on treating different types of CA-MRSA infection.Keywords: community, acquired methicillin, resistant Staphylococcus aureus, staphylococcal chromosomal cassette mec type, Panton-Valentine leukocidin, antibiotic resistance, pneumoniahttps://www.dovepress.com/molecular-epidemiology-of-community-acquired-methicillin-resistant-sta-peer-reviewed-fulltext-article-IDRcommunity-acquired methicillin-resistant staphylococcus aureusstaphylococcal chromosomal cassette mec typepanton-valentine leukocidinantibiotic resistancepneumonia |
| spellingShingle | Zhong J Wei M Yang C Yin Y Bai Y Li R Gu L Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection Infection and Drug Resistance community-acquired methicillin-resistant staphylococcus aureus staphylococcal chromosomal cassette mec type panton-valentine leukocidin antibiotic resistance pneumonia |
| title | Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection |
| title_full | Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection |
| title_fullStr | Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection |
| title_full_unstemmed | Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection |
| title_short | Molecular Epidemiology of Community-Acquired Methicillin-Resistant Staphylococcus aureus and Clinical Characteristics of Different Sites of Infection |
| title_sort | molecular epidemiology of community acquired methicillin resistant staphylococcus aureus and clinical characteristics of different sites of infection |
| topic | community-acquired methicillin-resistant staphylococcus aureus staphylococcal chromosomal cassette mec type panton-valentine leukocidin antibiotic resistance pneumonia |
| url | https://www.dovepress.com/molecular-epidemiology-of-community-acquired-methicillin-resistant-sta-peer-reviewed-fulltext-article-IDR |
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