The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa
INTRODUCTION: Pseudomonas aeruginosa (P. aeruginosa) is one of the primary opportunistic pathogens responsible for nosocomial infections. Aminoglycosides are an import ant component of antipseudomonal chemotherapy. The inactivation of drugs by modifying enzymes is the most common mechanism of aminog...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
2011-01-01
|
| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000900002 |
| _version_ | 1818254810919469056 |
|---|---|
| author | Farzam Vaziri Shahin Najar Peerayeh Qorban Behzadian Nejad Abbas Farhadian |
| author_facet | Farzam Vaziri Shahin Najar Peerayeh Qorban Behzadian Nejad Abbas Farhadian |
| author_sort | Farzam Vaziri |
| collection | DOAJ |
| description | INTRODUCTION: Pseudomonas aeruginosa (P. aeruginosa) is one of the primary opportunistic pathogens responsible for nosocomial infections. Aminoglycosides are an import ant component of antipseudomonal chemotherapy. The inactivation of drugs by modifying enzymes is the most common mechanism of aminoglycoside resistance. OBJECTIVES: The inactivation of aminoglycosides by modifying enzymes is the primary resistance mechanism employed by P. aeruginosa. The aim of the present study was to investigate the occurrence of aminoglycoside resistance and the prevalence of four import ant modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in P. aeruginosa in Iran. METHODS: A total of 250 clinical isolates of P. aeruginosa were collected from several hospitals in seven cities in Iran. Antimicrobial susceptibility tests (using the disk diffusion method and E-tests) were performed for all 250 isolates. In addition, all isolates were screened for the presence of modifying enzyme genes by polymerase chain reaction. RESULTS: The resistance rates, as determined by the disk diffusion method, were as follows: gentamicin 43%, tobramycin 38%, and amikacin 24%. Of the genes examined, aac (6')-II (36%) was the most frequently identified gene in phenotypic resist ant isolates, followed by ant (2")-I, aph (3')-VI, and aac (6')-I. CONCLUSIONS: Aminoglycoside resistance in P. aeruginosa remains a signific ant problem in Iran. Therefore, there is considerable local surveillance of aminoglycoside resistance. |
| first_indexed | 2024-12-12T17:01:53Z |
| format | Article |
| id | doaj.art-82f135b8fc8b4ec79a84e2f4c3fa323f |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-12-12T17:01:53Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-82f135b8fc8b4ec79a84e2f4c3fa323f2022-12-22T00:18:06ZengElsevier EspañaClinics1807-59321980-53222011-01-016691519152210.1590/S1807-59322011000900002The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosaFarzam VaziriShahin Najar PeerayehQorban Behzadian NejadAbbas FarhadianINTRODUCTION: Pseudomonas aeruginosa (P. aeruginosa) is one of the primary opportunistic pathogens responsible for nosocomial infections. Aminoglycosides are an import ant component of antipseudomonal chemotherapy. The inactivation of drugs by modifying enzymes is the most common mechanism of aminoglycoside resistance. OBJECTIVES: The inactivation of aminoglycosides by modifying enzymes is the primary resistance mechanism employed by P. aeruginosa. The aim of the present study was to investigate the occurrence of aminoglycoside resistance and the prevalence of four import ant modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in P. aeruginosa in Iran. METHODS: A total of 250 clinical isolates of P. aeruginosa were collected from several hospitals in seven cities in Iran. Antimicrobial susceptibility tests (using the disk diffusion method and E-tests) were performed for all 250 isolates. In addition, all isolates were screened for the presence of modifying enzyme genes by polymerase chain reaction. RESULTS: The resistance rates, as determined by the disk diffusion method, were as follows: gentamicin 43%, tobramycin 38%, and amikacin 24%. Of the genes examined, aac (6')-II (36%) was the most frequently identified gene in phenotypic resist ant isolates, followed by ant (2")-I, aph (3')-VI, and aac (6')-I. CONCLUSIONS: Aminoglycoside resistance in P. aeruginosa remains a signific ant problem in Iran. Therefore, there is considerable local surveillance of aminoglycoside resistance.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000900002Pseudomonas aeruginosaAntibioticResistanceaac (69)-IIant (20)-I |
| spellingShingle | Farzam Vaziri Shahin Najar Peerayeh Qorban Behzadian Nejad Abbas Farhadian The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa Clinics Pseudomonas aeruginosa Antibiotic Resistance aac (69)-II ant (20)-I |
| title | The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa |
| title_full | The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa |
| title_fullStr | The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa |
| title_full_unstemmed | The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa |
| title_short | The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa |
| title_sort | prevalence of aminoglycoside modifying enzyme genes aac 6 i aac 6 ii ant 2 i aph 3 vi in pseudomonas aeruginosa |
| topic | Pseudomonas aeruginosa Antibiotic Resistance aac (69)-II ant (20)-I |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000900002 |
| work_keys_str_mv | AT farzamvaziri theprevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT shahinnajarpeerayeh theprevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT qorbanbehzadiannejad theprevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT abbasfarhadian theprevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT farzamvaziri prevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT shahinnajarpeerayeh prevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT qorbanbehzadiannejad prevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa AT abbasfarhadian prevalenceofaminoglycosidemodifyingenzymegenesaac6iaac6iiant2iaph3viinpseudomonasaeruginosa |