High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot
Kiwifruit soft rot caused by the fungal pathogen Botryosphaeria dothidea is a serious disease in kiwifruit-growing regions worldwide. In this study, we reported the high-quality genome sequence of the highly virulent B. dothidea strain PTZ1 using PacBio Sequel techniques. In total, 100.87 million cl...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2021-04-01
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| Series: | PhytoFrontiers |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-07-20-0006-A |
| _version_ | 1797327239446003712 |
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| author | Kuan Liang Jianbin Lan Baoquan Wang Yuanyuan Liu Qi Lu Pu Liu |
| author_facet | Kuan Liang Jianbin Lan Baoquan Wang Yuanyuan Liu Qi Lu Pu Liu |
| author_sort | Kuan Liang |
| collection | DOAJ |
| description | Kiwifruit soft rot caused by the fungal pathogen Botryosphaeria dothidea is a serious disease in kiwifruit-growing regions worldwide. In this study, we reported the high-quality genome sequence of the highly virulent B. dothidea strain PTZ1 using PacBio Sequel techniques. In total, 100.87 million clean reads with mean read length of 9,871 bp were obtained. De novo assembly resulted in 28 contigs with a total size of 44.45 Mb. The GC content of the genome was 54.59%. Furthermore, genes related to specific virulence of the strain were identified, including 259 fungal cytochrome P450s, 550 carbohydrate-active enzymes, 860 secretory proteins, and 1,182 pathogen–host interactions related proteins. The genome is a useful resource to serve as a reference to facilitate the analysis of B. dothidea isolates and comparative genomic studies of the necrotroph pathogens.[Graphic: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-03-08T06:36:27Z |
| format | Article |
| id | doaj.art-82f99387d4fe4910abb50e16da9f9b7a |
| institution | Directory Open Access Journal |
| issn | 2690-5442 |
| language | English |
| last_indexed | 2024-03-08T06:36:27Z |
| publishDate | 2021-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj.art-82f99387d4fe4910abb50e16da9f9b7a2024-02-03T10:08:56ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422021-04-011212312510.1094/PHYTOFR-07-20-0006-AHigh-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft RotKuan Liang0Jianbin Lan1Baoquan Wang2Yuanyuan Liu3Qi Lu4Pu Liu5School of Horticulture, Anhui Agricultural University, Hefei 230036, People’s Republic of ChinaCollege of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160, People’s Republic of ChinaCollege of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, People’s Republic of ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, People’s Republic of ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, People’s Republic of ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, People’s Republic of ChinaKiwifruit soft rot caused by the fungal pathogen Botryosphaeria dothidea is a serious disease in kiwifruit-growing regions worldwide. In this study, we reported the high-quality genome sequence of the highly virulent B. dothidea strain PTZ1 using PacBio Sequel techniques. In total, 100.87 million clean reads with mean read length of 9,871 bp were obtained. De novo assembly resulted in 28 contigs with a total size of 44.45 Mb. The GC content of the genome was 54.59%. Furthermore, genes related to specific virulence of the strain were identified, including 259 fungal cytochrome P450s, 550 carbohydrate-active enzymes, 860 secretory proteins, and 1,182 pathogen–host interactions related proteins. The genome is a useful resource to serve as a reference to facilitate the analysis of B. dothidea isolates and comparative genomic studies of the necrotroph pathogens.[Graphic: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-07-20-0006-AkiwifruitBotryosphaeria dothideafungigenomehigh virulencesoft rot |
| spellingShingle | Kuan Liang Jianbin Lan Baoquan Wang Yuanyuan Liu Qi Lu Pu Liu High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot PhytoFrontiers kiwifruit Botryosphaeria dothidea fungi genome high virulence soft rot |
| title | High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot |
| title_full | High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot |
| title_fullStr | High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot |
| title_full_unstemmed | High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot |
| title_short | High-Quality Genome Resource of the Pathogen of Botryosphaeria dothidea Causing Kiwifruit Soft Rot |
| title_sort | high quality genome resource of the pathogen of botryosphaeria dothidea causing kiwifruit soft rot |
| topic | kiwifruit Botryosphaeria dothidea fungi genome high virulence soft rot |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-07-20-0006-A |
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