Purkinje cell microzones mediate distinct kinematics of a single movement
Abstract The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal su...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40111-5 |
| _version_ | 1797557950289543168 |
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| author | François G. C. Blot Joshua J. White Amy van Hattem Licia Scotti Vaishnavi Balaji Youri Adolfs R. Jeroen Pasterkamp Chris I. De Zeeuw Martijn Schonewille |
| author_facet | François G. C. Blot Joshua J. White Amy van Hattem Licia Scotti Vaishnavi Balaji Youri Adolfs R. Jeroen Pasterkamp Chris I. De Zeeuw Martijn Schonewille |
| author_sort | François G. C. Blot |
| collection | DOAJ |
| description | Abstract The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulations. Here, we demonstrate that its recently identified subpopulations of 9+ and 9- Purkinje cells exhibit an intermediate Aldolase C expression and electrophysiological profile, providing evidence for a graded continuum of intrinsic properties among PC subpopulations. By identifying and utilizing two Cre-lines that genetically target these floccular domains, we show with high spatial specificity that these subpopulations of Purkinje cells participate in separate micromodules with topographically organized connections. Finally, optogenetic excitation of the respective subpopulations results in movements around the same axis in space, yet with distinct kinematic profiles. These results indicate that Purkinje cell subpopulations integrate in discrete circuits and mediate particular parameters of single movements. |
| first_indexed | 2024-03-10T17:23:28Z |
| format | Article |
| id | doaj.art-82fd7fa96c6f48598a238a279fec019b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:23:28Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-82fd7fa96c6f48598a238a279fec019b2023-11-20T10:15:27ZengNature PortfolioNature Communications2041-17232023-07-0114111510.1038/s41467-023-40111-5Purkinje cell microzones mediate distinct kinematics of a single movementFrançois G. C. Blot0Joshua J. White1Amy van Hattem2Licia Scotti3Vaishnavi Balaji4Youri Adolfs5R. Jeroen Pasterkamp6Chris I. De Zeeuw7Martijn Schonewille8Department of Neuroscience, Erasmus MCDepartment of Neuroscience, Erasmus MCDepartment of Neuroscience, Erasmus MCDepartment of Neuroscience, Erasmus MCDepartment of Neuroscience, Erasmus MCDepartment of Translational Neuroscience, University Medical Center Utrecht, Brain Center, Utrecht UniversityDepartment of Translational Neuroscience, University Medical Center Utrecht, Brain Center, Utrecht UniversityDepartment of Neuroscience, Erasmus MCDepartment of Neuroscience, Erasmus MCAbstract The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulations. Here, we demonstrate that its recently identified subpopulations of 9+ and 9- Purkinje cells exhibit an intermediate Aldolase C expression and electrophysiological profile, providing evidence for a graded continuum of intrinsic properties among PC subpopulations. By identifying and utilizing two Cre-lines that genetically target these floccular domains, we show with high spatial specificity that these subpopulations of Purkinje cells participate in separate micromodules with topographically organized connections. Finally, optogenetic excitation of the respective subpopulations results in movements around the same axis in space, yet with distinct kinematic profiles. These results indicate that Purkinje cell subpopulations integrate in discrete circuits and mediate particular parameters of single movements.https://doi.org/10.1038/s41467-023-40111-5 |
| spellingShingle | François G. C. Blot Joshua J. White Amy van Hattem Licia Scotti Vaishnavi Balaji Youri Adolfs R. Jeroen Pasterkamp Chris I. De Zeeuw Martijn Schonewille Purkinje cell microzones mediate distinct kinematics of a single movement Nature Communications |
| title | Purkinje cell microzones mediate distinct kinematics of a single movement |
| title_full | Purkinje cell microzones mediate distinct kinematics of a single movement |
| title_fullStr | Purkinje cell microzones mediate distinct kinematics of a single movement |
| title_full_unstemmed | Purkinje cell microzones mediate distinct kinematics of a single movement |
| title_short | Purkinje cell microzones mediate distinct kinematics of a single movement |
| title_sort | purkinje cell microzones mediate distinct kinematics of a single movement |
| url | https://doi.org/10.1038/s41467-023-40111-5 |
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