Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System
Gelatin was immobilized selectively on the amide groups-modified bottom of a trench array of a photoresist template with 2 μm resolution by the ethyl(dimethylaminopropyl) carbodiimide/<i>N</i>-hydroxysuccinimide reaction. The gelatin-immobilized line array was brominated to generate a ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/23/4270 |
| _version_ | 1797507294268751872 |
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| author | Jem-Kun Chen Feng-Ping Lin Chi-Jung Chang Chien-Hsing Lu Chih-Feng Huang |
| author_facet | Jem-Kun Chen Feng-Ping Lin Chi-Jung Chang Chien-Hsing Lu Chih-Feng Huang |
| author_sort | Jem-Kun Chen |
| collection | DOAJ |
| description | Gelatin was immobilized selectively on the amide groups-modified bottom of a trench array of a photoresist template with 2 μm resolution by the ethyl(dimethylaminopropyl) carbodiimide/<i>N</i>-hydroxysuccinimide reaction. The gelatin-immobilized line array was brominated to generate a macroinitiator for atom transfer radical polymerization. Poly(methacrylic acid) (PMAA) brushes were grafted from the macroinitiator layer as line arrays of one-dimensional diffraction gratings (DGs) for various grafting polymerization times. A laser beam system was employed to analyze the optical feature with a characteristic diffraction effect of the PMAA DGs at a 45° incident angle along the transverse magnetic and transverse electric polarization. The growth of the PMAA brush lines increased both their heights and widths, leading to a change in the reflective diffraction intensity. The PMAA brushes under various grafting polymerization times were cleaved from the substrate by digestion of gelatin with trypsin, and their molecular weights were obtained by gel permeation chromatography. The change degree of the diffraction intensity varied linearly with the molecular weight of the PMAA brushes over a wide range, from 135 to 1475 kDa, with high correlation coefficients. Molecular weight determination of polymer brushes using the reflective diffraction intensity provides a simple method to monitor their growth in real time without polymer brush cleavage. |
| first_indexed | 2024-03-10T04:46:31Z |
| format | Article |
| id | doaj.art-82fdedb54f4741889b94e91ca229120b |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T04:46:31Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-82fdedb54f4741889b94e91ca229120b2023-11-23T02:55:25ZengMDPI AGPolymers2073-43602021-12-011323427010.3390/polym13234270Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser SystemJem-Kun Chen0Feng-Ping Lin1Chi-Jung Chang2Chien-Hsing Lu3Chih-Feng Huang4Department of Materials and Science Engineering, National Taiwan University of Science and Technology, 43, Sec. 4, Keelung Road, Taipei 106335, TaiwanDepartment of Materials and Science Engineering, National Taiwan University of Science and Technology, 43, Sec. 4, Keelung Road, Taipei 106335, TaiwanDepartment of Chemical Engineering, Feng Chia University, 100, Wenhwa Road, Taichung 40724, TaiwanDepartment of Obstetrics and Gynecology, Taichung Veterans General Hospital, Taichung 40705, Taiwani-Center for Advanced Science and Technology (iCAST), Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, TaiwanGelatin was immobilized selectively on the amide groups-modified bottom of a trench array of a photoresist template with 2 μm resolution by the ethyl(dimethylaminopropyl) carbodiimide/<i>N</i>-hydroxysuccinimide reaction. The gelatin-immobilized line array was brominated to generate a macroinitiator for atom transfer radical polymerization. Poly(methacrylic acid) (PMAA) brushes were grafted from the macroinitiator layer as line arrays of one-dimensional diffraction gratings (DGs) for various grafting polymerization times. A laser beam system was employed to analyze the optical feature with a characteristic diffraction effect of the PMAA DGs at a 45° incident angle along the transverse magnetic and transverse electric polarization. The growth of the PMAA brush lines increased both their heights and widths, leading to a change in the reflective diffraction intensity. The PMAA brushes under various grafting polymerization times were cleaved from the substrate by digestion of gelatin with trypsin, and their molecular weights were obtained by gel permeation chromatography. The change degree of the diffraction intensity varied linearly with the molecular weight of the PMAA brushes over a wide range, from 135 to 1475 kDa, with high correlation coefficients. Molecular weight determination of polymer brushes using the reflective diffraction intensity provides a simple method to monitor their growth in real time without polymer brush cleavage.https://www.mdpi.com/2073-4360/13/23/4270diffraction gratingPMAAmolecular weightlaser system |
| spellingShingle | Jem-Kun Chen Feng-Ping Lin Chi-Jung Chang Chien-Hsing Lu Chih-Feng Huang Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System Polymers diffraction grating PMAA molecular weight laser system |
| title | Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System |
| title_full | Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System |
| title_fullStr | Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System |
| title_full_unstemmed | Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System |
| title_short | Facile Molecular Weight Determination of Polymer Brushes Grafted from One-Dimensional Diffraction Grating by SI-ATRP Using Reflective Laser System |
| title_sort | facile molecular weight determination of polymer brushes grafted from one dimensional diffraction grating by si atrp using reflective laser system |
| topic | diffraction grating PMAA molecular weight laser system |
| url | https://www.mdpi.com/2073-4360/13/23/4270 |
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