Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing
Background:Streptococcus suis is a zoonotic pathogen that can cause severe infections such as meningitis and septicemia in both swine and humans. Rapid and accurate identification of the causative agent is very important for guiding clinical choices in administering countermeasures.Case Report: Here...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-12-01
|
| Series: | Frontiers in Public Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpubh.2019.00379/full |
| _version_ | 1818218786541535232 |
|---|---|
| author | Yuanyuan Dai Li Chen Wenjiao Chang Huaiwei Lu Peng Cui Xiaoling Ma |
| author_facet | Yuanyuan Dai Li Chen Wenjiao Chang Huaiwei Lu Peng Cui Xiaoling Ma |
| author_sort | Yuanyuan Dai |
| collection | DOAJ |
| description | Background:Streptococcus suis is a zoonotic pathogen that can cause severe infections such as meningitis and septicemia in both swine and humans. Rapid and accurate identification of the causative agent is very important for guiding clinical choices in administering countermeasures.Case Report: Here, we report a case of fatal S. suis infection in a patient who worked as a butcher in China. The 59-year-old man, who had previously undergone splenectomy, injured his finger while processing pork and developed severe sepsis. While blood cultures were negative following antibiotic treatment, S. suis was determined to be the causative agent by metagenomic next-generation sequencing (mNGS) and Sanger sequencing.Conclusion: Identification of etiological agents using techniques such as blood culture prior to antibiotic treatment is very important. mNGS may represent a useful method for diagnosis of infectious diseases, especially post-antibiotic treatment. |
| first_indexed | 2024-12-12T07:29:18Z |
| format | Article |
| id | doaj.art-82fe57f4c2894a778a62c3e0f40ac6b8 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-12-12T07:29:18Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-82fe57f4c2894a778a62c3e0f40ac6b82022-12-22T00:33:03ZengFrontiers Media S.A.Frontiers in Public Health2296-25652019-12-01710.3389/fpubh.2019.00379487139Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation SequencingYuanyuan Dai0Li Chen1Wenjiao Chang2Huaiwei Lu3Peng Cui4Xiaoling Ma5Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaDepartment of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaDepartment of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaDepartment of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaInfectious Disease Research and Development, Beijing Genomics Institute-Shanghai, Shanghai, ChinaDepartment of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, ChinaBackground:Streptococcus suis is a zoonotic pathogen that can cause severe infections such as meningitis and septicemia in both swine and humans. Rapid and accurate identification of the causative agent is very important for guiding clinical choices in administering countermeasures.Case Report: Here, we report a case of fatal S. suis infection in a patient who worked as a butcher in China. The 59-year-old man, who had previously undergone splenectomy, injured his finger while processing pork and developed severe sepsis. While blood cultures were negative following antibiotic treatment, S. suis was determined to be the causative agent by metagenomic next-generation sequencing (mNGS) and Sanger sequencing.Conclusion: Identification of etiological agents using techniques such as blood culture prior to antibiotic treatment is very important. mNGS may represent a useful method for diagnosis of infectious diseases, especially post-antibiotic treatment.https://www.frontiersin.org/article/10.3389/fpubh.2019.00379/fullStreptococcus suisbacteriastreptococcal toxic shock syndromemetagenomic next-generation sequencingblood culture |
| spellingShingle | Yuanyuan Dai Li Chen Wenjiao Chang Huaiwei Lu Peng Cui Xiaoling Ma Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing Frontiers in Public Health Streptococcus suis bacteria streptococcal toxic shock syndrome metagenomic next-generation sequencing blood culture |
| title | Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing |
| title_full | Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing |
| title_fullStr | Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing |
| title_full_unstemmed | Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing |
| title_short | Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing |
| title_sort | culture negative streptococcus suis infection diagnosed by metagenomic next generation sequencing |
| topic | Streptococcus suis bacteria streptococcal toxic shock syndrome metagenomic next-generation sequencing blood culture |
| url | https://www.frontiersin.org/article/10.3389/fpubh.2019.00379/full |
| work_keys_str_mv | AT yuanyuandai culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing AT lichen culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing AT wenjiaochang culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing AT huaiweilu culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing AT pengcui culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing AT xiaolingma culturenegativestreptococcussuisinfectiondiagnosedbymetagenomicnextgenerationsequencing |