High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina
Environmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. Between 2016 and 2017, 80 raw sewage samples and 8...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867022004317 |
| _version_ | 1827980000266027008 |
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| author | Ivana Lo Castro Carlos Espul Vanessa Salete de Paula Nancy R. Altabert Jorge E. Gonzalez Barbara Vieira Lago Livia Melo Villar |
| author_facet | Ivana Lo Castro Carlos Espul Vanessa Salete de Paula Nancy R. Altabert Jorge E. Gonzalez Barbara Vieira Lago Livia Melo Villar |
| author_sort | Ivana Lo Castro |
| collection | DOAJ |
| description | Environmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. Between 2016 and 2017, 80 raw sewage samples and 86 clinical samples (stool and serum) from suspected cases of hepatitis A and hepatitis E were obtained. HAV and HEV were tested by both real-time and nested PCR. Positive samples were sequenced for genotype determination and phylogenetic analysis. Overall, HAV was recovered in 39% of sewage samples and 61.1% of clinical samples. HEV was detected in 22.5% of sewage samples and 15.9% of clinical samples. HAV was found more frequently in sewage during the winter and in clinical samples in spring; HEV was more prevalent in sewage during summer and in clinical samples in autumn. All HAV isolates belonged to genotype IA and HEV isolates belonged to genotype 3, the most prevalent genotypes in South America. High prevalence of HAV and HEV in environmental and clinical samples in Mendoza, Argentina was observed. These findings reinforce the importance of environmental surveillance and implementation of health strategies to control the spread of HAV and HEV in developing countries. |
| first_indexed | 2024-04-09T21:45:42Z |
| format | Article |
| id | doaj.art-83024b147d9e4ad986db693ff16f6143 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-04-09T21:45:42Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-83024b147d9e4ad986db693ff16f61432023-03-25T05:10:29ZengElsevierBrazilian Journal of Infectious Diseases1413-86702023-03-01272102738High prevalence of hepatitis A and E viruses in environmental and clinical samples from West ArgentinaIvana Lo Castro0Carlos Espul1Vanessa Salete de Paula2Nancy R. Altabert3Jorge E. Gonzalez4Barbara Vieira Lago5Livia Melo Villar6Virology Section, Central Hospital Mendoza, ArgentinaVirology Section, Central Hospital Mendoza, ArgentinaLaboratório de Virologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, BrazilNational Reference Laboratory of Viral Hepatitis, National Institute of Infectious Diseases, ANLIS “Carlos G. Malbrán”, Buenos Aires, ArgentinaNational Reference Laboratory of Viral Hepatitis, National Institute of Infectious Diseases, ANLIS “Carlos G. Malbrán”, Buenos Aires, ArgentinaLaboratory of Viral Hepatitis, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil; Instituto de Tecnologia em Imunobiológicos – Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil; Corresponding author.Laboratory of Viral Hepatitis, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, BrazilEnvironmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. Between 2016 and 2017, 80 raw sewage samples and 86 clinical samples (stool and serum) from suspected cases of hepatitis A and hepatitis E were obtained. HAV and HEV were tested by both real-time and nested PCR. Positive samples were sequenced for genotype determination and phylogenetic analysis. Overall, HAV was recovered in 39% of sewage samples and 61.1% of clinical samples. HEV was detected in 22.5% of sewage samples and 15.9% of clinical samples. HAV was found more frequently in sewage during the winter and in clinical samples in spring; HEV was more prevalent in sewage during summer and in clinical samples in autumn. All HAV isolates belonged to genotype IA and HEV isolates belonged to genotype 3, the most prevalent genotypes in South America. High prevalence of HAV and HEV in environmental and clinical samples in Mendoza, Argentina was observed. These findings reinforce the importance of environmental surveillance and implementation of health strategies to control the spread of HAV and HEV in developing countries.http://www.sciencedirect.com/science/article/pii/S1413867022004317HAVHEVGenetic diversitySewageClinical samples |
| spellingShingle | Ivana Lo Castro Carlos Espul Vanessa Salete de Paula Nancy R. Altabert Jorge E. Gonzalez Barbara Vieira Lago Livia Melo Villar High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina Brazilian Journal of Infectious Diseases HAV HEV Genetic diversity Sewage Clinical samples |
| title | High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina |
| title_full | High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina |
| title_fullStr | High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina |
| title_full_unstemmed | High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina |
| title_short | High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina |
| title_sort | high prevalence of hepatitis a and e viruses in environmental and clinical samples from west argentina |
| topic | HAV HEV Genetic diversity Sewage Clinical samples |
| url | http://www.sciencedirect.com/science/article/pii/S1413867022004317 |
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