Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis
This study aimed to establish a cell-based assay (CBA) for the detection of agrin antibodies (Agrin-Ab) to explore the clinical features of agrin antibody-positive Chinese patients with myasthenia gravis (Agrin-MG). We developed a CBA based on the human full-length agrin protein expressed in HEK293T...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.753247/full |
| _version_ | 1818570371102670848 |
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| author | Shumin Wang Haonan Yang Haonan Yang Rongjing Guo Lulu Wang Lulu Wang Yingna Zhang Jie Lv Xue Zhao Jing Zhang Hua Fang Qingyong Zhang Yunke Zhang Junhong Yang Xinzheng Cui Peiyang Gao Ting Chang Feng Gao |
| author_facet | Shumin Wang Haonan Yang Haonan Yang Rongjing Guo Lulu Wang Lulu Wang Yingna Zhang Jie Lv Xue Zhao Jing Zhang Hua Fang Qingyong Zhang Yunke Zhang Junhong Yang Xinzheng Cui Peiyang Gao Ting Chang Feng Gao |
| author_sort | Shumin Wang |
| collection | DOAJ |
| description | This study aimed to establish a cell-based assay (CBA) for the detection of agrin antibodies (Agrin-Ab) to explore the clinical features of agrin antibody-positive Chinese patients with myasthenia gravis (Agrin-MG). We developed a CBA based on the human full-length agrin protein expressed in HEK293T cells for the reliable and efficient detection of Agrin-Ab. Clinical data and serum samples were collected from 1948 MG patients in 26 provinces in China. The demographic and clinical features of Agrin-MG patients were compared with those of other MG patient subsets. Eighteen Agrin-MG cases were identified from 1948 MG patients. Nine patients were Agrin-Ab positive, and nine were AChR-Ab and Agrin-Ab double-positive (Agrin/AChR-MG). Eleven (61.11%) patients were males older than 40 years of age. The initial symptom in 13 (81.25%) cases was ocular weakness. Occasionally, the initial symptom was limb-girdle weakness (two cases) or bulbar muscle weakness (one case). Agrin-MG patients demonstrated slight improvement following treatment with either acetylcholinesterase inhibitor or prednisone; however, the combination of the two drugs could effectively relieve MG symptoms. In China, Agrin-MG demonstrated seropositivity rates of 0.92%. These patients were commonly middle-aged or elderly men. The patients usually presented weakness in the ocular, bulbar, and limb muscles, which may be combined with thymoma. These patients have more severe diseases, although the combination of pyridostigmine and prednisone was usually effective in relieving symptoms. |
| first_indexed | 2024-12-14T13:40:27Z |
| format | Article |
| id | doaj.art-830423f5ec534e87bb8e355fd3296bf6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T13:40:27Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-830423f5ec534e87bb8e355fd3296bf62022-12-21T22:59:28ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-12-011210.3389/fimmu.2021.753247753247Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia GravisShumin Wang0Haonan Yang1Haonan Yang2Rongjing Guo3Lulu Wang4Lulu Wang5Yingna Zhang6Jie Lv7Xue Zhao8Jing Zhang9Hua Fang10Qingyong Zhang11Yunke Zhang12Junhong Yang13Xinzheng Cui14Peiyang Gao15Ting Chang16Feng Gao17Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaBGI College, Zhengzhou University, Zhengzhou, ChinaDepartment of Neurology, Tangdu Hospital, The Air Force Medical University, Xi’an, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaMyasthenia Gravis Comprehensive Diagnosis and Treatment Center, Henan Provincial People’s Hospital, Zhengzhou, ChinaDepartment of Encephalopathy, First Affiliated Hospital of Henan University of Traditional Chinese Medicine (TCM), Zhengzhou, ChinaDepartment of Encephalopathy, First Affiliated Hospital of Henan University of Traditional Chinese Medicine (TCM), Zhengzhou, ChinaMyasthenia Gravis Comprehensive Diagnosis and Treatment Center, Henan Provincial People’s Hospital, Zhengzhou, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaDepartment of Neurology, Tangdu Hospital, The Air Force Medical University, Xi’an, ChinaDepartment of Neuroimmunology, Henan Institute of Medical and Pharmaceutical Sciences, Academy of Medical Science, Zhengzhou University, Zhengzhou, ChinaThis study aimed to establish a cell-based assay (CBA) for the detection of agrin antibodies (Agrin-Ab) to explore the clinical features of agrin antibody-positive Chinese patients with myasthenia gravis (Agrin-MG). We developed a CBA based on the human full-length agrin protein expressed in HEK293T cells for the reliable and efficient detection of Agrin-Ab. Clinical data and serum samples were collected from 1948 MG patients in 26 provinces in China. The demographic and clinical features of Agrin-MG patients were compared with those of other MG patient subsets. Eighteen Agrin-MG cases were identified from 1948 MG patients. Nine patients were Agrin-Ab positive, and nine were AChR-Ab and Agrin-Ab double-positive (Agrin/AChR-MG). Eleven (61.11%) patients were males older than 40 years of age. The initial symptom in 13 (81.25%) cases was ocular weakness. Occasionally, the initial symptom was limb-girdle weakness (two cases) or bulbar muscle weakness (one case). Agrin-MG patients demonstrated slight improvement following treatment with either acetylcholinesterase inhibitor or prednisone; however, the combination of the two drugs could effectively relieve MG symptoms. In China, Agrin-MG demonstrated seropositivity rates of 0.92%. These patients were commonly middle-aged or elderly men. The patients usually presented weakness in the ocular, bulbar, and limb muscles, which may be combined with thymoma. These patients have more severe diseases, although the combination of pyridostigmine and prednisone was usually effective in relieving symptoms.https://www.frontiersin.org/articles/10.3389/fimmu.2021.753247/fullmyasthenia graviscell-based assayagrinautoantibodyclinical features |
| spellingShingle | Shumin Wang Haonan Yang Haonan Yang Rongjing Guo Lulu Wang Lulu Wang Yingna Zhang Jie Lv Xue Zhao Jing Zhang Hua Fang Qingyong Zhang Yunke Zhang Junhong Yang Xinzheng Cui Peiyang Gao Ting Chang Feng Gao Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis Frontiers in Immunology myasthenia gravis cell-based assay agrin autoantibody clinical features |
| title | Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis |
| title_full | Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis |
| title_fullStr | Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis |
| title_full_unstemmed | Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis |
| title_short | Antibodies to Full-Length Agrin Protein in Chinese Patients With Myasthenia Gravis |
| title_sort | antibodies to full length agrin protein in chinese patients with myasthenia gravis |
| topic | myasthenia gravis cell-based assay agrin autoantibody clinical features |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.753247/full |
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