Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective
The association between plasma cholesterol levels and the development of dementia continues to be an important topic of discussion in the scientific community, while the results in the literature vary significantly. We study the effect of reducing oxidized neuronal cholesterol on the lipid raft stru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2905 |
| _version_ | 1797570035905986560 |
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| author | Maher A. Dayeh George Livadiotis Farzan Aminian Kwan H. Cheng James L. Roberts Nikita Viswasam Saber Elaydi |
| author_facet | Maher A. Dayeh George Livadiotis Farzan Aminian Kwan H. Cheng James L. Roberts Nikita Viswasam Saber Elaydi |
| author_sort | Maher A. Dayeh |
| collection | DOAJ |
| description | The association between plasma cholesterol levels and the development of dementia continues to be an important topic of discussion in the scientific community, while the results in the literature vary significantly. We study the effect of reducing oxidized neuronal cholesterol on the lipid raft structure of plasma membrane. The levels of plasma membrane cholesterol were reduced by treating the intact cells with methyl-ß-cyclodextrin (MßCD). The relationship between the cell viability with varying levels of MßCD was then examined. The viability curves are well described by a modified form of the empirical Gompertz law of mortality. A detailed statistical analysis is performed on the fitting results, showing that increasing MßCD concentration has a minor, rather than significant, effect on the cellular viability. In particular, the dependence of viability on MßCD concentration was found to be characterized by a ~25% increase per 1 μM of MßCD concentration. |
| first_indexed | 2024-03-10T20:19:22Z |
| format | Article |
| id | doaj.art-830635597a7d4d4d85fb7c5f6ee2aae5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:19:22Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-830635597a7d4d4d85fb7c5f6ee2aae52023-11-19T22:17:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218290510.3390/ijms21082905Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis PerspectiveMaher A. Dayeh0George Livadiotis1Farzan Aminian2Kwan H. Cheng3James L. Roberts4Nikita Viswasam5Saber Elaydi6Space Science and Engineering Division, Southwest Research Institute, San Antonio, TX 78238, USASpace Science and Engineering Division, Southwest Research Institute, San Antonio, TX 78238, USANeuroscience Program, Departments of Biology, Mathematics, Engineering and Physics & Astronomy, Trinity University, San Antonio, TX 78212, USANeuroscience Program, Departments of Biology, Mathematics, Engineering and Physics & Astronomy, Trinity University, San Antonio, TX 78212, USANeuroscience Program, Departments of Biology, Mathematics, Engineering and Physics & Astronomy, Trinity University, San Antonio, TX 78212, USANeuroscience Program, Departments of Biology, Mathematics, Engineering and Physics & Astronomy, Trinity University, San Antonio, TX 78212, USANeuroscience Program, Departments of Biology, Mathematics, Engineering and Physics & Astronomy, Trinity University, San Antonio, TX 78212, USAThe association between plasma cholesterol levels and the development of dementia continues to be an important topic of discussion in the scientific community, while the results in the literature vary significantly. We study the effect of reducing oxidized neuronal cholesterol on the lipid raft structure of plasma membrane. The levels of plasma membrane cholesterol were reduced by treating the intact cells with methyl-ß-cyclodextrin (MßCD). The relationship between the cell viability with varying levels of MßCD was then examined. The viability curves are well described by a modified form of the empirical Gompertz law of mortality. A detailed statistical analysis is performed on the fitting results, showing that increasing MßCD concentration has a minor, rather than significant, effect on the cellular viability. In particular, the dependence of viability on MßCD concentration was found to be characterized by a ~25% increase per 1 μM of MßCD concentration.https://www.mdpi.com/1422-0067/21/8/2905dementiaAlzheimer’s diseaseoxidative stresscellular viability |
| spellingShingle | Maher A. Dayeh George Livadiotis Farzan Aminian Kwan H. Cheng James L. Roberts Nikita Viswasam Saber Elaydi Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective International Journal of Molecular Sciences dementia Alzheimer’s disease oxidative stress cellular viability |
| title | Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective |
| title_full | Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective |
| title_fullStr | Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective |
| title_full_unstemmed | Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective |
| title_short | Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective |
| title_sort | effects of cholesterol in stress related neuronal death a statistical analysis perspective |
| topic | dementia Alzheimer’s disease oxidative stress cellular viability |
| url | https://www.mdpi.com/1422-0067/21/8/2905 |
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