Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers
Zinc is a trace mineral of interest for optimizing growth in feedlot cattle due to its roles in many physiological functions, including growth. Twenty-four Angus-cross steers (467 ± 13 kg) were used to assess the effects of supplemental Zn and ractopamine hydrochloride (RAC) on trace mineral concent...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Animal Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2023.1191230/full |
| _version_ | 1797799500216008704 |
|---|---|
| author | Emma L. Rients Remy N. Wyatt Erin L. Deters Olivia N. Genther-Schroeder Stephanie L. Hansen |
| author_facet | Emma L. Rients Remy N. Wyatt Erin L. Deters Olivia N. Genther-Schroeder Stephanie L. Hansen |
| author_sort | Emma L. Rients |
| collection | DOAJ |
| description | Zinc is a trace mineral of interest for optimizing growth in feedlot cattle due to its roles in many physiological functions, including growth. Twenty-four Angus-cross steers (467 ± 13 kg) were used to assess the effects of supplemental Zn and ractopamine hydrochloride (RAC) on trace mineral concentrations and muscle gene expression. Four GrowSafe-equipped pens were randomly assigned to treatments (1 pen of six steers/treatment): 0 (CON), 60 (LOW), 120 (MED) or 180 (HI) mg supplemental Zn/kg DM (Availa-Zn, Zinpro). Dietary Zn treatments were initiated on d 0 and RAC supplementation (300 mg·steer·-1·d-1; Actogain45, Zoetis) began on d 53. Blood, liver and muscle (longissimus thoracis) samples were collected from all steers on d -4, 48, and 67. The LOW treatment was removed from gene expression analyses due to < 3 steers being represented for 14 of 22 genes. Data were analyzed using ProcMixed of SAS with the fixed effect of treatment and steer as the experimental unit; orthogonal linear and quadratic contrast statements were used to compare treatments. On d 48 and 67, there were linear and quadratic trends for plasma Zn to be greater in Zn-supplemented steers than CON (P ≤ 0.10). On d 48, there was a tendency for a quadratic decrease on the expression of SLC30A4 (P ≤ 0.07) but no other differences due to treatment. On d 67, several genes involved in Zn transport and storage (MTA1, SLC39A7, SLC39A8, SLC39A9, SLC39A10, SLC39A13) were decreased (P ≤ 0.08), suggesting increased growth influences intracellular Zn trafficking and demands. |
| first_indexed | 2024-03-13T04:20:51Z |
| format | Article |
| id | doaj.art-8307f70290c8479ca9aee8fa232fae1f |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-03-13T04:20:51Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Animal Science |
| spelling | doaj.art-8307f70290c8479ca9aee8fa232fae1f2023-06-20T10:15:11ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252023-06-01410.3389/fanim.2023.11912301191230Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steersEmma L. RientsRemy N. WyattErin L. DetersOlivia N. Genther-SchroederStephanie L. HansenZinc is a trace mineral of interest for optimizing growth in feedlot cattle due to its roles in many physiological functions, including growth. Twenty-four Angus-cross steers (467 ± 13 kg) were used to assess the effects of supplemental Zn and ractopamine hydrochloride (RAC) on trace mineral concentrations and muscle gene expression. Four GrowSafe-equipped pens were randomly assigned to treatments (1 pen of six steers/treatment): 0 (CON), 60 (LOW), 120 (MED) or 180 (HI) mg supplemental Zn/kg DM (Availa-Zn, Zinpro). Dietary Zn treatments were initiated on d 0 and RAC supplementation (300 mg·steer·-1·d-1; Actogain45, Zoetis) began on d 53. Blood, liver and muscle (longissimus thoracis) samples were collected from all steers on d -4, 48, and 67. The LOW treatment was removed from gene expression analyses due to < 3 steers being represented for 14 of 22 genes. Data were analyzed using ProcMixed of SAS with the fixed effect of treatment and steer as the experimental unit; orthogonal linear and quadratic contrast statements were used to compare treatments. On d 48 and 67, there were linear and quadratic trends for plasma Zn to be greater in Zn-supplemented steers than CON (P ≤ 0.10). On d 48, there was a tendency for a quadratic decrease on the expression of SLC30A4 (P ≤ 0.07) but no other differences due to treatment. On d 67, several genes involved in Zn transport and storage (MTA1, SLC39A7, SLC39A8, SLC39A9, SLC39A10, SLC39A13) were decreased (P ≤ 0.08), suggesting increased growth influences intracellular Zn trafficking and demands.https://www.frontiersin.org/articles/10.3389/fanim.2023.1191230/fullbeef cattlegene expressionmuscleractopamine hydrochloridezinczinc transport |
| spellingShingle | Emma L. Rients Remy N. Wyatt Erin L. Deters Olivia N. Genther-Schroeder Stephanie L. Hansen Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers Frontiers in Animal Science beef cattle gene expression muscle ractopamine hydrochloride zinc zinc transport |
| title | Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| title_full | Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| title_fullStr | Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| title_full_unstemmed | Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| title_short | Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| title_sort | zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers |
| topic | beef cattle gene expression muscle ractopamine hydrochloride zinc zinc transport |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2023.1191230/full |
| work_keys_str_mv | AT emmalrients zincsupplementationandractopaminehydrochlorideimpactgeneexpressionofzinctransportersinfinishingbeefsteers AT remynwyatt zincsupplementationandractopaminehydrochlorideimpactgeneexpressionofzinctransportersinfinishingbeefsteers AT erinldeters zincsupplementationandractopaminehydrochlorideimpactgeneexpressionofzinctransportersinfinishingbeefsteers AT oliviangentherschroeder zincsupplementationandractopaminehydrochlorideimpactgeneexpressionofzinctransportersinfinishingbeefsteers AT stephanielhansen zincsupplementationandractopaminehydrochlorideimpactgeneexpressionofzinctransportersinfinishingbeefsteers |