Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells.
Euglena gracilis Z (Euglena) is a unicellular, photosynthesizing, microscopic green alga. It contains several nutrients such as vitamins, minerals, and unsaturated fatty acids. In this study, to verify the potential role of Euglena consumption on human health and obesity, we evaluated the effect of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5813920?pdf=render |
| _version_ | 1818957476139106304 |
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| author | Ryota Sugimoto Naoko Ishibashi-Ohgo Kohei Atsuji Yuko Miwa Osamu Iwata Ayaka Nakashima Kengo Suzuki |
| author_facet | Ryota Sugimoto Naoko Ishibashi-Ohgo Kohei Atsuji Yuko Miwa Osamu Iwata Ayaka Nakashima Kengo Suzuki |
| author_sort | Ryota Sugimoto |
| collection | DOAJ |
| description | Euglena gracilis Z (Euglena) is a unicellular, photosynthesizing, microscopic green alga. It contains several nutrients such as vitamins, minerals, and unsaturated fatty acids. In this study, to verify the potential role of Euglena consumption on human health and obesity, we evaluated the effect of Euglena on human adipose-derived stem cells. We prepared a Euglena extract and evaluated its effect on cell growth and lipid accumulation, and found that cell growth was promoted by the addition of the Euglena extract. Interestingly, intracellular lipid accumulation was inhibited in a concentration-dependent manner. Quantitative real-time PCR analysis and western blotting analysis indicated that the Euglena extract suppressed adipocyte differentiation by inhibiting the gene expression of the master regulators peroxisome proliferator-activated receptor-γ (PPARγ) and one of three CCAAT-enhancer-binding proteins (C/EBPα). Further Oil Red O staining experiments indicated that the Euglena extract inhibited the early stage of adipocyte-differentiation. Consistent with these results, we observed that down-regulation of gene expression was involved in the early stage of adipogenesis represented by the sterol regulatory element binding protein 1 c (SREBP1c), two of three CCAAT-enhancer-binding proteins (C/EBPβ, C/EBPδ), and the cAMP regulatory element-binding protein (CREB). Taken together, these data suggest that Euglena extract is a promising candidate for the development of a new therapeutic treatment for obesity. |
| first_indexed | 2024-12-20T11:10:27Z |
| format | Article |
| id | doaj.art-830f27115b2443d383eda30980de4e30 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T11:10:27Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-830f27115b2443d383eda30980de4e302022-12-21T19:42:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01132e019240410.1371/journal.pone.0192404Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells.Ryota SugimotoNaoko Ishibashi-OhgoKohei AtsujiYuko MiwaOsamu IwataAyaka NakashimaKengo SuzukiEuglena gracilis Z (Euglena) is a unicellular, photosynthesizing, microscopic green alga. It contains several nutrients such as vitamins, minerals, and unsaturated fatty acids. In this study, to verify the potential role of Euglena consumption on human health and obesity, we evaluated the effect of Euglena on human adipose-derived stem cells. We prepared a Euglena extract and evaluated its effect on cell growth and lipid accumulation, and found that cell growth was promoted by the addition of the Euglena extract. Interestingly, intracellular lipid accumulation was inhibited in a concentration-dependent manner. Quantitative real-time PCR analysis and western blotting analysis indicated that the Euglena extract suppressed adipocyte differentiation by inhibiting the gene expression of the master regulators peroxisome proliferator-activated receptor-γ (PPARγ) and one of three CCAAT-enhancer-binding proteins (C/EBPα). Further Oil Red O staining experiments indicated that the Euglena extract inhibited the early stage of adipocyte-differentiation. Consistent with these results, we observed that down-regulation of gene expression was involved in the early stage of adipogenesis represented by the sterol regulatory element binding protein 1 c (SREBP1c), two of three CCAAT-enhancer-binding proteins (C/EBPβ, C/EBPδ), and the cAMP regulatory element-binding protein (CREB). Taken together, these data suggest that Euglena extract is a promising candidate for the development of a new therapeutic treatment for obesity.http://europepmc.org/articles/PMC5813920?pdf=render |
| spellingShingle | Ryota Sugimoto Naoko Ishibashi-Ohgo Kohei Atsuji Yuko Miwa Osamu Iwata Ayaka Nakashima Kengo Suzuki Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. PLoS ONE |
| title | Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. |
| title_full | Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. |
| title_fullStr | Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. |
| title_full_unstemmed | Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. |
| title_short | Euglena extract suppresses adipocyte-differentiation in human adipose-derived stem cells. |
| title_sort | euglena extract suppresses adipocyte differentiation in human adipose derived stem cells |
| url | http://europepmc.org/articles/PMC5813920?pdf=render |
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