Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism
The study aimed to investigate the influence of obesity on cellular features of equine endometrial progenitor cells (Eca EPCs), including viability, proliferation capacity, mitochondrial metabolism, and oxidative homeostasis. Eca EPCs derived from non-obese (non-OB) and obese (OB) mares were charact...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/9/1437 |
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| author | Agnieszka Smieszek Klaudia Marcinkowska Ariadna Pielok Mateusz Sikora Lukas Valihrach Elaine Carnevale Krzysztof Marycz |
| author_facet | Agnieszka Smieszek Klaudia Marcinkowska Ariadna Pielok Mateusz Sikora Lukas Valihrach Elaine Carnevale Krzysztof Marycz |
| author_sort | Agnieszka Smieszek |
| collection | DOAJ |
| description | The study aimed to investigate the influence of obesity on cellular features of equine endometrial progenitor cells (Eca EPCs), including viability, proliferation capacity, mitochondrial metabolism, and oxidative homeostasis. Eca EPCs derived from non-obese (non-OB) and obese (OB) mares were characterized by cellular phenotype and multipotency. Obesity-induced changes in the activity of Eca EPCs include the decline of their proliferative activity, clonogenic potential, mitochondrial metabolism, and enhanced oxidative stress. Eca EPCs isolated from obese mares were characterized by an increased occurrence of early apoptosis, loss of mitochondrial dynamics, and senescence-associated phenotype. Attenuated metabolism of Eca EPCs OB was related to increased expression of pro-apoptotic markers (CASP9, BAX, P53, P21), enhanced expression of OPN, PI3K, and AKT, simultaneously with decreased signaling stabilizing cellular homeostasis (including mitofusin, SIRT1, FOXP3). Obesity alters functional features and the self-renewal potential of endometrial progenitor cells. The impaired cytophysiology of progenitor cells from obese endometrium predicts lower regenerative capacity if used as autologous transplants. |
| first_indexed | 2024-03-10T04:17:05Z |
| format | Article |
| id | doaj.art-830f9ffed2ca4914b0b6c105c1a3b87e |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:17:05Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-830f9ffed2ca4914b0b6c105c1a3b87e2023-11-23T07:59:13ZengMDPI AGCells2073-44092022-04-01119143710.3390/cells11091437Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial MetabolismAgnieszka Smieszek0Klaudia Marcinkowska1Ariadna Pielok2Mateusz Sikora3Lukas Valihrach4Elaine Carnevale5Krzysztof Marycz6Department of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, PolandDepartment of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, PolandDepartment of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, PolandDepartment of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, PolandLaboratory of Gene Expression, Institute of Biotechnology CAS, Biocev, 25250 Vestec, Czech RepublicEquine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Colins, CO 80523-1693, USADepartment of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, PolandThe study aimed to investigate the influence of obesity on cellular features of equine endometrial progenitor cells (Eca EPCs), including viability, proliferation capacity, mitochondrial metabolism, and oxidative homeostasis. Eca EPCs derived from non-obese (non-OB) and obese (OB) mares were characterized by cellular phenotype and multipotency. Obesity-induced changes in the activity of Eca EPCs include the decline of their proliferative activity, clonogenic potential, mitochondrial metabolism, and enhanced oxidative stress. Eca EPCs isolated from obese mares were characterized by an increased occurrence of early apoptosis, loss of mitochondrial dynamics, and senescence-associated phenotype. Attenuated metabolism of Eca EPCs OB was related to increased expression of pro-apoptotic markers (CASP9, BAX, P53, P21), enhanced expression of OPN, PI3K, and AKT, simultaneously with decreased signaling stabilizing cellular homeostasis (including mitofusin, SIRT1, FOXP3). Obesity alters functional features and the self-renewal potential of endometrial progenitor cells. The impaired cytophysiology of progenitor cells from obese endometrium predicts lower regenerative capacity if used as autologous transplants.https://www.mdpi.com/2073-4409/11/9/1437obesityendometrial progenitor cellscellular metabolismself-renewal potential |
| spellingShingle | Agnieszka Smieszek Klaudia Marcinkowska Ariadna Pielok Mateusz Sikora Lukas Valihrach Elaine Carnevale Krzysztof Marycz Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism Cells obesity endometrial progenitor cells cellular metabolism self-renewal potential |
| title | Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism |
| title_full | Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism |
| title_fullStr | Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism |
| title_full_unstemmed | Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism |
| title_short | Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism |
| title_sort | obesity affects the proliferative potential of equine endometrial progenitor cells and modulates their molecular phenotype associated with mitochondrial metabolism |
| topic | obesity endometrial progenitor cells cellular metabolism self-renewal potential |
| url | https://www.mdpi.com/2073-4409/11/9/1437 |
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