Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/10/5/333 |
| _version_ | 1797567299117383680 |
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| author | Chao-Ju Chen Li-Ling Hsieh Shu-Kai Lin Chu-Feng Wang Yi-Hui Huang Shang-Yi Lin Po-Liang Lu |
| author_facet | Chao-Ju Chen Li-Ling Hsieh Shu-Kai Lin Chu-Feng Wang Yi-Hui Huang Shang-Yi Lin Po-Liang Lu |
| author_sort | Chao-Ju Chen |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs. |
| first_indexed | 2024-03-10T19:40:43Z |
| format | Article |
| id | doaj.art-830fe9636dfa4c2e900e107ac7038625 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T19:40:43Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-830fe9636dfa4c2e900e107ac70386252023-11-20T01:18:54ZengMDPI AGDiagnostics2075-44182020-05-0110533310.3390/diagnostics10050333Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely DiagnosisChao-Ju Chen0Li-Ling Hsieh1Shu-Kai Lin2Chu-Feng Wang3Yi-Hui Huang4Shang-Yi Lin5Po-Liang Lu6Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanCoronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.https://www.mdpi.com/2075-4418/10/5/333coronavirus disease 2019 (COVID-19)severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)molecular diagnosticsreal-time reverse transcriptase polymerase chain (RT-PCR) |
| spellingShingle | Chao-Ju Chen Li-Ling Hsieh Shu-Kai Lin Chu-Feng Wang Yi-Hui Huang Shang-Yi Lin Po-Liang Lu Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis Diagnostics coronavirus disease 2019 (COVID-19) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) molecular diagnostics real-time reverse transcriptase polymerase chain (RT-PCR) |
| title | Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis |
| title_full | Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis |
| title_fullStr | Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis |
| title_full_unstemmed | Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis |
| title_short | Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis |
| title_sort | optimization of the cdc protocol of molecular diagnosis of covid 19 for timely diagnosis |
| topic | coronavirus disease 2019 (COVID-19) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) molecular diagnostics real-time reverse transcriptase polymerase chain (RT-PCR) |
| url | https://www.mdpi.com/2075-4418/10/5/333 |
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