Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine
Emerging RNA virus infections are a growing concern among domestic poultry industries due to the severe impact they can have on flock health and economic livelihoods. Avian paramyxoviruses (APMV; avulaviruses, AaV) are pathogenic, negative-sense RNA viruses that cause serious infections in the respi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/3/699 |
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| author | Amy C. Klink Oleksandr Rula Mykola Sushko Maksym Bezymennyi Oleksandr Mezinov Oleksandr Gaidash Xiao Bai Anton Stegniy Maryna Sapachova Roman Datsenko Sergiy Skorokhod Vitalii Nedosekov Nichola J. Hill Levan Ninua Ganna Kovalenko Anne Lise Ducluzeau Andriy Mezhenskyi Jeremy Buttler Devin M. Drown Douglas Causey Borys Stegniy Anton Gerilovych Eric Bortz Denys Muzyka |
| author_facet | Amy C. Klink Oleksandr Rula Mykola Sushko Maksym Bezymennyi Oleksandr Mezinov Oleksandr Gaidash Xiao Bai Anton Stegniy Maryna Sapachova Roman Datsenko Sergiy Skorokhod Vitalii Nedosekov Nichola J. Hill Levan Ninua Ganna Kovalenko Anne Lise Ducluzeau Andriy Mezhenskyi Jeremy Buttler Devin M. Drown Douglas Causey Borys Stegniy Anton Gerilovych Eric Bortz Denys Muzyka |
| author_sort | Amy C. Klink |
| collection | DOAJ |
| description | Emerging RNA virus infections are a growing concern among domestic poultry industries due to the severe impact they can have on flock health and economic livelihoods. Avian paramyxoviruses (APMV; avulaviruses, AaV) are pathogenic, negative-sense RNA viruses that cause serious infections in the respiratory and central nervous systems. APMV was detected in multiple avian species during the 2017 wild bird migration season in Ukraine and studied using PCR, virus isolation, and sequencing. Of 4090 wild bird samples collected, mostly from southern Ukraine, eleven isolates were grown in ovo and identified for APMV serotype by hemagglutinin inhibition test as: APMV-1, APMV-4, APMV-6, and APMV-7. To build One Health’s capacity to characterize APMV virulence and analyze the potential risks of spillover to immunologically naïve populations, we sequenced virus genomes in veterinary research labs in Ukraine using a nanopore (MinION) platform. RNA was extracted and amplified using a multiplex tiling primer approach to specifically capture full-length APMV-1 (<i>n</i> = 5) and APMV-6 (<i>n</i> = 2) genomes at high read depth. All APMV-1 and APMV-6 fusion (F) proteins possessed a monobasic cleavage site, suggesting these APMVs were likely low virulence, annually circulating strains. Utilization of this low-cost method will identify gaps in viral evolution and circulation in this understudied but important critical region for Eurasia. |
| first_indexed | 2024-03-11T05:47:13Z |
| format | Article |
| id | doaj.art-8310193f344e40a1a07ed706c20302a4 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T05:47:13Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-8310193f344e40a1a07ed706c20302a42023-11-17T14:23:05ZengMDPI AGViruses1999-49152023-03-0115369910.3390/v15030699Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern UkraineAmy C. Klink0Oleksandr Rula1Mykola Sushko2Maksym Bezymennyi3Oleksandr Mezinov4Oleksandr Gaidash5Xiao Bai6Anton Stegniy7Maryna Sapachova8Roman Datsenko9Sergiy Skorokhod10Vitalii Nedosekov11Nichola J. Hill12Levan Ninua13Ganna Kovalenko14Anne Lise Ducluzeau15Andriy Mezhenskyi16Jeremy Buttler17Devin M. Drown18Douglas Causey19Borys Stegniy20Anton Gerilovych21Eric Bortz22Denys Muzyka23Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USANational Scientific Center Institute of Experimental and Clinical Veterinary Medicine, 61023 Kharkiv, UkraineState Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 03151 Kyiv, UkraineInstitute for Veterinary Medicine, National Academy of Agrarian Sciences, 03151 Kyiv, UkraineThe F.E. Falz-Fein Biosphere Reserve “Askania Nova”, Askania-Nova, 75230 Kakhovka Raion, UkraineInstitute of Natural Sciences, Department of Zoology, H.S. Skovoroda Kharkiv National Pedagogical University, 61022 Kharkiv, UkraineDepartment of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USANational Scientific Center Institute of Experimental and Clinical Veterinary Medicine, 61023 Kharkiv, UkraineState Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 03151 Kyiv, UkraineState Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 03151 Kyiv, UkraineState Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 03151 Kyiv, UkraineDepartment of Epizootology, The National University of Life and Environmental Science of Ukraine, 03041 Kyiv, UkraineDepartment of Biology, University of Massachusetts, Boston, MA 02125, USAInstitute of Ecology, Ilia State University, Tbilisi 0162, GeorgiaDepartment of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USADepartment of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USAState Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 03151 Kyiv, UkraineDepartment of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USADepartment of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USADepartment of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USANational Scientific Center Institute of Experimental and Clinical Veterinary Medicine, 61023 Kharkiv, UkraineNational Scientific Center Institute of Experimental and Clinical Veterinary Medicine, 61023 Kharkiv, UkraineDepartment of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USANational Scientific Center Institute of Experimental and Clinical Veterinary Medicine, 61023 Kharkiv, UkraineEmerging RNA virus infections are a growing concern among domestic poultry industries due to the severe impact they can have on flock health and economic livelihoods. Avian paramyxoviruses (APMV; avulaviruses, AaV) are pathogenic, negative-sense RNA viruses that cause serious infections in the respiratory and central nervous systems. APMV was detected in multiple avian species during the 2017 wild bird migration season in Ukraine and studied using PCR, virus isolation, and sequencing. Of 4090 wild bird samples collected, mostly from southern Ukraine, eleven isolates were grown in ovo and identified for APMV serotype by hemagglutinin inhibition test as: APMV-1, APMV-4, APMV-6, and APMV-7. To build One Health’s capacity to characterize APMV virulence and analyze the potential risks of spillover to immunologically naïve populations, we sequenced virus genomes in veterinary research labs in Ukraine using a nanopore (MinION) platform. RNA was extracted and amplified using a multiplex tiling primer approach to specifically capture full-length APMV-1 (<i>n</i> = 5) and APMV-6 (<i>n</i> = 2) genomes at high read depth. All APMV-1 and APMV-6 fusion (F) proteins possessed a monobasic cleavage site, suggesting these APMVs were likely low virulence, annually circulating strains. Utilization of this low-cost method will identify gaps in viral evolution and circulation in this understudied but important critical region for Eurasia.https://www.mdpi.com/1999-4915/15/3/699viral ecologysurveillance of avian paramyxovirusesAPMVwild birdsnext-generation sequencingminion |
| spellingShingle | Amy C. Klink Oleksandr Rula Mykola Sushko Maksym Bezymennyi Oleksandr Mezinov Oleksandr Gaidash Xiao Bai Anton Stegniy Maryna Sapachova Roman Datsenko Sergiy Skorokhod Vitalii Nedosekov Nichola J. Hill Levan Ninua Ganna Kovalenko Anne Lise Ducluzeau Andriy Mezhenskyi Jeremy Buttler Devin M. Drown Douglas Causey Borys Stegniy Anton Gerilovych Eric Bortz Denys Muzyka Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine Viruses viral ecology surveillance of avian paramyxoviruses APMV wild birds next-generation sequencing minion |
| title | Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine |
| title_full | Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine |
| title_fullStr | Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine |
| title_full_unstemmed | Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine |
| title_short | Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine |
| title_sort | discovery of avian paramyxoviruses apmv 1 and apmv 6 in shorebirds and waterfowl in southern ukraine |
| topic | viral ecology surveillance of avian paramyxoviruses APMV wild birds next-generation sequencing minion |
| url | https://www.mdpi.com/1999-4915/15/3/699 |
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