Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress
Morchella sextelata was used as experimental material to explore the effects of NaCl stress on mycelia growth and physiological indexes. The mycelial growth index, mycelial morphology, biomass, mycelial membrane permeability, osmotic regulatory substance content and antioxidant enzymes of M. sextela...
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| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010064 |
| _version_ | 1811308314188840960 |
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| author | Jing SUN Tong YANG Zhihao ZHANG Jianrui WANG Yu LIU |
| author_facet | Jing SUN Tong YANG Zhihao ZHANG Jianrui WANG Yu LIU |
| author_sort | Jing SUN |
| collection | DOAJ |
| description | Morchella sextelata was used as experimental material to explore the effects of NaCl stress on mycelia growth and physiological indexes. The mycelial growth index, mycelial morphology, biomass, mycelial membrane permeability, osmotic regulatory substance content and antioxidant enzymes of M. sextelata were measured under different concentrations of NaCl (0, 100, 200, 300, 400 and 500 mmol/L). The effects of different levels of NaCl stress on mycelial growth and resistance physiology of M. sextelata were analyzed. Correlation analysis and principal component analysis were used to screen the key physiological indexes during the mycelial growth of M. sextelata under different NaCl concentrations. The results indicated that as the concentration of NaCl in the medium increased, the mycelial growth rate, mycelial biomass, mycelial diameter and intercellular polysaccharide (IPS) content of M. sextelata showed a downward trend, while the osmotic pressure (OP) and electrical conductivity (EC) showed an upward trend. The soluble protein content, malondialdehyde (MDA) content, proline (Pro) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity and catalase (CAT) activity all increased first and then decreased with the increasing of NaCl concentration. At 200 mmol/L NaCl concentration, the malondialdehyde (MDA) content, peroxidase (POD) activity and catalase (CAT) activity peaked, proline content and SOD activity peaked at 300 mmol/L salt concentration, while soluble protein content peaked at 400 mmol/L NaCl concentration. Through principal component analysis, the accumulative contribution rate of the first three principal components was 96.611%. Among them, when NaCl concentration was 0~100 mmol/L, the indicative physiological indexes were growth rate, mycelial diameter, dry weight, fresh weight and IPS content. When NaCl concentration was 100~200 mmol/L, the indicative physiological indexes were MDA content, soluble protein content, SOD activity, POD activity and CAT activity. When NaCl concentration was 200~400 mmol/L, the indicative physiological indexes were Pro content, soluble protein content and SOD activity. |
| first_indexed | 2024-04-13T09:21:00Z |
| format | Article |
| id | doaj.art-8310f8b621ff4f15af271000d7e76a2a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:21:00Z |
| publishDate | 2022-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-8310f8b621ff4f15af271000d7e76a2a2022-12-22T02:52:36ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-11-01432215516410.13386/j.issn1002-0306.20220100642022010064-22Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl StressJing SUN0Tong YANG1Zhihao ZHANG2Jianrui WANG3Yu LIU4Shandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, ChinaShandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, ChinaShandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, ChinaShandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, ChinaShandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, ChinaMorchella sextelata was used as experimental material to explore the effects of NaCl stress on mycelia growth and physiological indexes. The mycelial growth index, mycelial morphology, biomass, mycelial membrane permeability, osmotic regulatory substance content and antioxidant enzymes of M. sextelata were measured under different concentrations of NaCl (0, 100, 200, 300, 400 and 500 mmol/L). The effects of different levels of NaCl stress on mycelial growth and resistance physiology of M. sextelata were analyzed. Correlation analysis and principal component analysis were used to screen the key physiological indexes during the mycelial growth of M. sextelata under different NaCl concentrations. The results indicated that as the concentration of NaCl in the medium increased, the mycelial growth rate, mycelial biomass, mycelial diameter and intercellular polysaccharide (IPS) content of M. sextelata showed a downward trend, while the osmotic pressure (OP) and electrical conductivity (EC) showed an upward trend. The soluble protein content, malondialdehyde (MDA) content, proline (Pro) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity and catalase (CAT) activity all increased first and then decreased with the increasing of NaCl concentration. At 200 mmol/L NaCl concentration, the malondialdehyde (MDA) content, peroxidase (POD) activity and catalase (CAT) activity peaked, proline content and SOD activity peaked at 300 mmol/L salt concentration, while soluble protein content peaked at 400 mmol/L NaCl concentration. Through principal component analysis, the accumulative contribution rate of the first three principal components was 96.611%. Among them, when NaCl concentration was 0~100 mmol/L, the indicative physiological indexes were growth rate, mycelial diameter, dry weight, fresh weight and IPS content. When NaCl concentration was 100~200 mmol/L, the indicative physiological indexes were MDA content, soluble protein content, SOD activity, POD activity and CAT activity. When NaCl concentration was 200~400 mmol/L, the indicative physiological indexes were Pro content, soluble protein content and SOD activity.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010064morchella sextelatanacl stressgrowth rateantioxidant enzymemalondialdehydeosmotic pressurecorrelation analysisprincipal component analysis |
| spellingShingle | Jing SUN Tong YANG Zhihao ZHANG Jianrui WANG Yu LIU Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress Shipin gongye ke-ji morchella sextelata nacl stress growth rate antioxidant enzyme malondialdehyde osmotic pressure correlation analysis principal component analysis |
| title | Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress |
| title_full | Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress |
| title_fullStr | Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress |
| title_full_unstemmed | Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress |
| title_short | Physiological Response and Principal Component Analysis of Morchella sextelata Mycelia under NaCl Stress |
| title_sort | physiological response and principal component analysis of morchella sextelata mycelia under nacl stress |
| topic | morchella sextelata nacl stress growth rate antioxidant enzyme malondialdehyde osmotic pressure correlation analysis principal component analysis |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010064 |
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