Riboflavin content in autofluorescent earthworm coelomocytes is species-specific.
We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2007-01-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4560 |
| _version_ | 1811241586983436288 |
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| author | Joanna Homa Barbara Płytycz Beata Kozioł Małgorzata Rózanowska A John Morgan |
| author_facet | Joanna Homa Barbara Płytycz Beata Kozioł Małgorzata Rózanowska A John Morgan |
| author_sort | Joanna Homa |
| collection | DOAJ |
| description | We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2) was identified as a major fluorophore in Eisenia jetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes) from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy. |
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| format | Article |
| id | doaj.art-8311fcb925b2417aa58ddb38b60afc4c |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-04-12T13:38:24Z |
| publishDate | 2007-01-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-8311fcb925b2417aa58ddb38b60afc4c2022-12-22T03:30:55ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312007-01-0144427528010.5603/4560Riboflavin content in autofluorescent earthworm coelomocytes is species-specific.Joanna HomaBarbara PłytyczBeata KoziołMałgorzata RózanowskaA John MorganWe have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2) was identified as a major fluorophore in Eisenia jetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes) from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy.http://czasopisma.viamedica.pl/fhc/article/view/4560 |
| spellingShingle | Joanna Homa Barbara Płytycz Beata Kozioł Małgorzata Rózanowska A John Morgan Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. Folia Histochemica et Cytobiologica |
| title | Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. |
| title_full | Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. |
| title_fullStr | Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. |
| title_full_unstemmed | Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. |
| title_short | Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. |
| title_sort | riboflavin content in autofluorescent earthworm coelomocytes is species specific |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4560 |
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