NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD)
Abstract Each year, bovine respiratory disease (BRD) results in significant economic loss in the cattle sector, and novel metabolic profiling for early diagnosis represents a promising tool for developing effective measures for disease management. Here, 1H-nuclear magnetic resonance (1H-NMR) spectra...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29234-3 |
| _version_ | 1797864993120583680 |
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| author | Mariana Santos-Rivera Nicholas C. Fitzkee Rebecca A. Hill Richard E. Baird Ellianna Blair Merrilee Thoresen Amelia R. Woolums Florencia Meyer Carrie K. Vance |
| author_facet | Mariana Santos-Rivera Nicholas C. Fitzkee Rebecca A. Hill Richard E. Baird Ellianna Blair Merrilee Thoresen Amelia R. Woolums Florencia Meyer Carrie K. Vance |
| author_sort | Mariana Santos-Rivera |
| collection | DOAJ |
| description | Abstract Each year, bovine respiratory disease (BRD) results in significant economic loss in the cattle sector, and novel metabolic profiling for early diagnosis represents a promising tool for developing effective measures for disease management. Here, 1H-nuclear magnetic resonance (1H-NMR) spectra were used to characterize metabolites from blood plasma collected from male dairy calves (n = 10) intentionally infected with two of the main BRD causal agents, bovine respiratory syncytial virus (BRSV) and Mannheimia haemolytica (MH), to generate a well-defined metabolomic profile under controlled conditions. In response to infection, 46 metabolites (BRSV = 32, MH = 33) changed in concentration compared to the uninfected state. Fuel substrates and products exhibited a particularly strong effect, reflecting imbalances that occur during the immune response. Furthermore, 1H-NMR spectra from samples from the uninfected and infected stages were discriminated with an accuracy, sensitivity, and specificity ≥ 95% using chemometrics to model the changes associated with disease, suggesting that metabolic profiles can be used for further development, understanding, and validation of novel diagnostic tools. |
| first_indexed | 2024-04-09T23:00:51Z |
| format | Article |
| id | doaj.art-83121f39568f4b7097e60e8d2612393b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T23:00:51Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-83121f39568f4b7097e60e8d2612393b2023-03-22T10:59:40ZengNature PortfolioScientific Reports2045-23222023-02-0113111710.1038/s41598-023-29234-3NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD)Mariana Santos-Rivera0Nicholas C. Fitzkee1Rebecca A. Hill2Richard E. Baird3Ellianna Blair4Merrilee Thoresen5Amelia R. Woolums6Florencia Meyer7Carrie K. Vance8Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State UniversityDepartment of Chemistry, Mississippi State UniversityDepartment of Chemistry, Mississippi State UniversityDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State UniversityDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State UniversityCollege of Veterinary Medicine, Pathobiology and Population Medicine, Mississippi State UniversityCollege of Veterinary Medicine, Pathobiology and Population Medicine, Mississippi State UniversityDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State UniversityDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State UniversityAbstract Each year, bovine respiratory disease (BRD) results in significant economic loss in the cattle sector, and novel metabolic profiling for early diagnosis represents a promising tool for developing effective measures for disease management. Here, 1H-nuclear magnetic resonance (1H-NMR) spectra were used to characterize metabolites from blood plasma collected from male dairy calves (n = 10) intentionally infected with two of the main BRD causal agents, bovine respiratory syncytial virus (BRSV) and Mannheimia haemolytica (MH), to generate a well-defined metabolomic profile under controlled conditions. In response to infection, 46 metabolites (BRSV = 32, MH = 33) changed in concentration compared to the uninfected state. Fuel substrates and products exhibited a particularly strong effect, reflecting imbalances that occur during the immune response. Furthermore, 1H-NMR spectra from samples from the uninfected and infected stages were discriminated with an accuracy, sensitivity, and specificity ≥ 95% using chemometrics to model the changes associated with disease, suggesting that metabolic profiles can be used for further development, understanding, and validation of novel diagnostic tools.https://doi.org/10.1038/s41598-023-29234-3 |
| spellingShingle | Mariana Santos-Rivera Nicholas C. Fitzkee Rebecca A. Hill Richard E. Baird Ellianna Blair Merrilee Thoresen Amelia R. Woolums Florencia Meyer Carrie K. Vance NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) Scientific Reports |
| title | NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) |
| title_full | NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) |
| title_fullStr | NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) |
| title_full_unstemmed | NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) |
| title_short | NMR-based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease (BRD) |
| title_sort | nmr based metabolomics of plasma from dairy calves infected with two primary causal agents of bovine respiratory disease brd |
| url | https://doi.org/10.1038/s41598-023-29234-3 |
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