Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras
The phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of p...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/3/490 |
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| author | Krystyna Żyżyńska-Galeńska Agnieszka Bernat Anna Piliszek Jolanta Karasiewicz Ewa Szablisty Mariusz Sacharczuk Marta Brewińska-Olchowik Michał Bochenek Joanna Grabarek Jacek Andrzej Modliński |
| author_facet | Krystyna Żyżyńska-Galeńska Agnieszka Bernat Anna Piliszek Jolanta Karasiewicz Ewa Szablisty Mariusz Sacharczuk Marta Brewińska-Olchowik Michał Bochenek Joanna Grabarek Jacek Andrzej Modliński |
| author_sort | Krystyna Żyżyńska-Galeńska |
| collection | DOAJ |
| description | The phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency. |
| first_indexed | 2024-03-09T00:33:33Z |
| format | Article |
| id | doaj.art-831274efe9df4a8ca54308cc8efb354b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T00:33:33Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-831274efe9df4a8ca54308cc8efb354b2023-12-11T18:21:50ZengMDPI AGCells2073-44092021-02-0110349010.3390/cells10030490Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult ChimaerasKrystyna Żyżyńska-Galeńska0Agnieszka Bernat1Anna Piliszek2Jolanta Karasiewicz3Ewa Szablisty4Mariusz Sacharczuk5Marta Brewińska-Olchowik6Michał Bochenek7Joanna Grabarek8Jacek Andrzej Modliński9Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandDepartment of Experimental Genomics, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandLaboratory of Cytometry, Nencki Institute of Experimental Biology, 02-093 Warsaw, PolandDepartment of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 32-083 Balice, PolandFaculty of Biology, Medicine and Health, Division of Developmental Biology & Medicine, University of Manchester, Manchester, M13 9PT, UKDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, PolandThe phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency.https://www.mdpi.com/2073-4409/10/3/490reprogrammingembryonic nichechimaeraplasticity |
| spellingShingle | Krystyna Żyżyńska-Galeńska Agnieszka Bernat Anna Piliszek Jolanta Karasiewicz Ewa Szablisty Mariusz Sacharczuk Marta Brewińska-Olchowik Michał Bochenek Joanna Grabarek Jacek Andrzej Modliński Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras Cells reprogramming embryonic niche chimaera plasticity |
| title | Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras |
| title_full | Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras |
| title_fullStr | Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras |
| title_full_unstemmed | Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras |
| title_short | Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras |
| title_sort | embryonic environmental niche reprograms somatic cells to express pluripotency markers and participate in adult chimaeras |
| topic | reprogramming embryonic niche chimaera plasticity |
| url | https://www.mdpi.com/2073-4409/10/3/490 |
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