Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary
Most picornaviruses of the family <i>Picornaviridae</i> are relatively well known, but there are certain “neglected” genera like <i>Bopivirus</i>, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, th...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/1/66 |
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| author | Zoltán László Péter Pankovics Gábor Reuter Attila Cságola Ádám Bálint Mihály Albert Ákos Boros |
| author_facet | Zoltán László Péter Pankovics Gábor Reuter Attila Cságola Ádám Bálint Mihály Albert Ákos Boros |
| author_sort | Zoltán László |
| collection | DOAJ |
| description | Most picornaviruses of the family <i>Picornaviridae</i> are relatively well known, but there are certain “neglected” genera like <i>Bopivirus</i>, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and <i>cre</i>-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species <i>Bopivirus A</i>, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus <i>Bopivirus</i>. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses. |
| first_indexed | 2024-03-10T13:27:11Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T13:27:11Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-8312f2af71844b75aacb7c174a3921992023-11-21T08:49:29ZengMDPI AGViruses1999-49152021-01-011316610.3390/v13010066Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in HungaryZoltán László0Péter Pankovics1Gábor Reuter2Attila Cságola3Ádám Bálint4Mihály Albert5Ákos Boros6Department of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, HungaryCeva Phylaxia Ltd., H-1107 Budapest, HungaryDepartment of Virology, National Food Chain Safety Office Veterinary Diagnostic Directorate, H-1143 Budapest, HungaryCeva Phylaxia Ltd., H-1107 Budapest, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, HungaryMost picornaviruses of the family <i>Picornaviridae</i> are relatively well known, but there are certain “neglected” genera like <i>Bopivirus</i>, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and <i>cre</i>-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species <i>Bopivirus A</i>, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus <i>Bopivirus</i>. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses.https://www.mdpi.com/1999-4915/13/1/66picornavirusIRESbopiviruslivestockepidemiologyovine |
| spellingShingle | Zoltán László Péter Pankovics Gábor Reuter Attila Cságola Ádám Bálint Mihály Albert Ákos Boros Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary Viruses picornavirus IRES bopivirus livestock epidemiology ovine |
| title | Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary |
| title_full | Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary |
| title_fullStr | Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary |
| title_full_unstemmed | Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary |
| title_short | Multiple Types of Novel Enteric Bopiviruses (<i>Picornaviridae</i>) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary |
| title_sort | multiple types of novel enteric bopiviruses i picornaviridae i with the possibility of interspecies transmission identified from cloven hoofed domestic livestock ovine caprine and bovine in hungary |
| topic | picornavirus IRES bopivirus livestock epidemiology ovine |
| url | https://www.mdpi.com/1999-4915/13/1/66 |
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