Role of lncRNA LIPE-AS1 in adipogenesis
Recent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specif...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Adipocyte |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21623945.2021.2013415 |
| _version_ | 1818968175443705856 |
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| author | Alyssa Thunen Deirdre La Placa Zhifang Zhang John E. Shively |
| author_facet | Alyssa Thunen Deirdre La Placa Zhifang Zhang John E. Shively |
| author_sort | Alyssa Thunen |
| collection | DOAJ |
| description | Recent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specific expression of isoforms of the murine lncRNA were found in liver and adipose tissue, one of which, designated mLas-V3, overlapped the Lipe gene encoding hormone-sensitive lipase in both mouse and man suggesting that it may have a functional role in adipose tissue. Knock down of expression of mLas-V3 using anti-sense oligos (ASOs) led to a significant decrease in the differentiation of the OP9 pre-adipocyte cell line through the down regulation of the major adipogenic transcription factors Pparg and Cebpa. Knock down of mLas-V3 induced apoptosis during the differentiation of OP9 cells as shown by expression of active caspase-3, a change in the localization of LIP/LAP isoforms of C/EBPβ, and expression of the cellular stress induced factors CHOP, p53, PUMA, and NOXA. We conclude that mLas-V3 may play a role in protecting against stress associated with adipogenesis, and its absence leads to apoptosis. |
| first_indexed | 2024-12-20T14:00:31Z |
| format | Article |
| id | doaj.art-831330b5d4904bf1a0c51aca96a322f6 |
| institution | Directory Open Access Journal |
| issn | 2162-3945 2162-397X |
| language | English |
| last_indexed | 2024-12-20T14:00:31Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj.art-831330b5d4904bf1a0c51aca96a322f62022-12-21T19:38:22ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2022-12-01111112710.1080/21623945.2021.20134152013415Role of lncRNA LIPE-AS1 in adipogenesisAlyssa Thunen0Deirdre La Placa1Zhifang Zhang2John E. Shively3Beckman Research Institute of City of HopeBeckman Research Institute of City of HopeBeckman Research Institute of City of HopeBeckman Research Institute of City of HopeRecent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specific expression of isoforms of the murine lncRNA were found in liver and adipose tissue, one of which, designated mLas-V3, overlapped the Lipe gene encoding hormone-sensitive lipase in both mouse and man suggesting that it may have a functional role in adipose tissue. Knock down of expression of mLas-V3 using anti-sense oligos (ASOs) led to a significant decrease in the differentiation of the OP9 pre-adipocyte cell line through the down regulation of the major adipogenic transcription factors Pparg and Cebpa. Knock down of mLas-V3 induced apoptosis during the differentiation of OP9 cells as shown by expression of active caspase-3, a change in the localization of LIP/LAP isoforms of C/EBPβ, and expression of the cellular stress induced factors CHOP, p53, PUMA, and NOXA. We conclude that mLas-V3 may play a role in protecting against stress associated with adipogenesis, and its absence leads to apoptosis.http://dx.doi.org/10.1080/21623945.2021.2013415lipeadipogenesislong non-coding rnaceacam1apoptosis |
| spellingShingle | Alyssa Thunen Deirdre La Placa Zhifang Zhang John E. Shively Role of lncRNA LIPE-AS1 in adipogenesis Adipocyte lipe adipogenesis long non-coding rna ceacam1 apoptosis |
| title | Role of lncRNA LIPE-AS1 in adipogenesis |
| title_full | Role of lncRNA LIPE-AS1 in adipogenesis |
| title_fullStr | Role of lncRNA LIPE-AS1 in adipogenesis |
| title_full_unstemmed | Role of lncRNA LIPE-AS1 in adipogenesis |
| title_short | Role of lncRNA LIPE-AS1 in adipogenesis |
| title_sort | role of lncrna lipe as1 in adipogenesis |
| topic | lipe adipogenesis long non-coding rna ceacam1 apoptosis |
| url | http://dx.doi.org/10.1080/21623945.2021.2013415 |
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