Molecular Detection of the Crown Gall Disease Caused by an Agrobacterium Species in Tunisian Fruit Trees' Nurseries
The importance of the crown gall disease caused by bacteria of the genus Agrobacterium was assessed for 13 isolates and 5 plant tissues belonging to 4 different species. Samples were collected from different growing areas (soil, plants and tumor tissues). The aim of this study was to isolate the bac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institution of the Agricultural Research and Higher Education
2015-06-01
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| Series: | Tunisian Journal of Plant Protection |
| Subjects: | |
| Online Access: | http://www.tjpp.tn/SiteWeb/PreviousIssues/TJPP10-1/01Chaouachi.pdf
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| Summary: | The importance of the crown gall disease caused by bacteria of the genus Agrobacterium was assessed
for 13 isolates and 5 plant tissues belonging to 4 different species. Samples were collected from
different growing areas (soil, plants and tumor tissues). The aim of this study was to isolate the
bacterium from symptomatic plants, soils and for the first time from asymptomatic plant tissues and to
identify new isolates of Agrobacterium sp. by sequence analysis of relevant marker genes. Two
primers' pairs were used to test the incidence of crown gall disease in Tunisian nurseries. The first
targeted the virulence region within the Ti plasmid and the second the polygalacturonase gene used for
the identification of Allorhizobium vitis. Simple PCR and sequencing unambiguously demonstrated the
unexpected contamination of the asymptomatic plant tissues by part of the Ti plasmid (virulence
region). The samples were clustered according to the BLAST analyses of a sequenced vir gene region
and phylogenetic trees allowed the distinction of two main groups, the first group carries vitopine type
Ti plasmids, the second group carries nopaline, octopine, type Ti plasmids agropine, mikimopine,
cucumopine type Ri plasmid and others Ri plasmid types. The BLAST analyses of a polygalacturonase
encoding gene sequence from 6 isolates and 3 strains showed 95-99% identity to the sequences of
Allorhizobium vitis previously deposited in NCBI GenBank database. |
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| ISSN: | 1737-5436 2490-4368 |