AdapterRemoval: easy cleaning of next-generation sequencing reads
<p>Abstract</p> <p>Background</p> <p>With the advent of next-generation sequencing there is an increased demand for tools to pre-process and handle the vast amounts of data generated. One recurring problem is adapter contamination in the reads, i.e. the partial or compl...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1756-0500/5/337 |
| _version_ | 1819026841664487424 |
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| author | Lindgreen Stinus |
| author_facet | Lindgreen Stinus |
| author_sort | Lindgreen Stinus |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>With the advent of next-generation sequencing there is an increased demand for tools to pre-process and handle the vast amounts of data generated. One recurring problem is adapter contamination in the reads, i.e. the partial or complete sequencing of adapter sequences. These adapter sequences have to be removed as they can hinder correct mapping of the reads and influence SNP calling and other downstream analyses.</p> <p>Findings</p> <p>We present a tool called AdapterRemoval which is able to pre-process both single and paired-end data. The program locates and removes adapter residues from the reads, it is able to combine paired reads if they overlap, and it can optionally trim low-quality nucleotides. Furthermore, it can look for adapter sequence in both the 5’ and 3’ ends of the reads. This is a flexible tool that can be tuned to accommodate different experimental settings and sequencing platforms producing FASTQ files. AdapterRemoval is shown to be good at trimming adapters from both single-end and paired-end data.</p> <p>Conclusions</p> <p>AdapterRemoval is a comprehensive tool for analyzing next-generation sequencing data. It exhibits good performance both in terms of sensitivity and specificity. AdapterRemoval has already been used in various large projects and it is possible to extend it further to accommodate application-specific biases in the data.</p> |
| first_indexed | 2024-12-21T05:32:59Z |
| format | Article |
| id | doaj.art-83196b1ddd8149d5b4a54a2676f0418b |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-21T05:32:59Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-83196b1ddd8149d5b4a54a2676f0418b2022-12-21T19:14:30ZengBMCBMC Research Notes1756-05002012-07-015133710.1186/1756-0500-5-337AdapterRemoval: easy cleaning of next-generation sequencing readsLindgreen Stinus<p>Abstract</p> <p>Background</p> <p>With the advent of next-generation sequencing there is an increased demand for tools to pre-process and handle the vast amounts of data generated. One recurring problem is adapter contamination in the reads, i.e. the partial or complete sequencing of adapter sequences. These adapter sequences have to be removed as they can hinder correct mapping of the reads and influence SNP calling and other downstream analyses.</p> <p>Findings</p> <p>We present a tool called AdapterRemoval which is able to pre-process both single and paired-end data. The program locates and removes adapter residues from the reads, it is able to combine paired reads if they overlap, and it can optionally trim low-quality nucleotides. Furthermore, it can look for adapter sequence in both the 5’ and 3’ ends of the reads. This is a flexible tool that can be tuned to accommodate different experimental settings and sequencing platforms producing FASTQ files. AdapterRemoval is shown to be good at trimming adapters from both single-end and paired-end data.</p> <p>Conclusions</p> <p>AdapterRemoval is a comprehensive tool for analyzing next-generation sequencing data. It exhibits good performance both in terms of sensitivity and specificity. AdapterRemoval has already been used in various large projects and it is possible to extend it further to accommodate application-specific biases in the data.</p>http://www.biomedcentral.com/1756-0500/5/337Next-generation sequencingAdapter trimmingData pre-processingSequence alignmentPaired-end readsSingle-end reads |
| spellingShingle | Lindgreen Stinus AdapterRemoval: easy cleaning of next-generation sequencing reads BMC Research Notes Next-generation sequencing Adapter trimming Data pre-processing Sequence alignment Paired-end reads Single-end reads |
| title | AdapterRemoval: easy cleaning of next-generation sequencing reads |
| title_full | AdapterRemoval: easy cleaning of next-generation sequencing reads |
| title_fullStr | AdapterRemoval: easy cleaning of next-generation sequencing reads |
| title_full_unstemmed | AdapterRemoval: easy cleaning of next-generation sequencing reads |
| title_short | AdapterRemoval: easy cleaning of next-generation sequencing reads |
| title_sort | adapterremoval easy cleaning of next generation sequencing reads |
| topic | Next-generation sequencing Adapter trimming Data pre-processing Sequence alignment Paired-end reads Single-end reads |
| url | http://www.biomedcentral.com/1756-0500/5/337 |
| work_keys_str_mv | AT lindgreenstinus adapterremovaleasycleaningofnextgenerationsequencingreads |