Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry
Due to the increasing pharmaceutical interest of oligonucleotides, for example in antisense therapy and vaccines, their analytical characterization is of fundamental importance due to their complex structure. For this purpose, mass spectrometry is a viable tool for structural studies of nucleic acid...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/3/1169 |
| _version_ | 1797623771448737792 |
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| author | Christopher Gawlig Michael Rühl |
| author_facet | Christopher Gawlig Michael Rühl |
| author_sort | Christopher Gawlig |
| collection | DOAJ |
| description | Due to the increasing pharmaceutical interest of oligonucleotides, for example in antisense therapy and vaccines, their analytical characterization is of fundamental importance due to their complex structure. For this purpose, mass spectrometry is a viable tool for structural studies of nucleic acids. Structural information regarding the primary sequence of a nucleic acid can reliably be gained via tandem mass spectrometry (MSMS) fragmentation. In this work, we present the characteristic fragmentation behavior of short-chain oligonucleotides (15–35 nucleotides) with respect to the collision-induced dissociation (CID) voltage used. The relationship and influence of the length of the oligonucleotide and its charge state is also discussed. The results presented here can be helpful for estimating the required fragmentation energies of short-chain oligonucleotides and their sequencing. |
| first_indexed | 2024-03-11T09:33:29Z |
| format | Article |
| id | doaj.art-831bcd5296ef4b5faa216e14f035246e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:29Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-831bcd5296ef4b5faa216e14f035246e2023-11-16T17:28:38ZengMDPI AGMolecules1420-30492023-01-01283116910.3390/molecules28031169Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass SpectrometryChristopher Gawlig0Michael Rühl1Biospring GmbH, 60386 Frankfurt am Main, GermanyBiospring GmbH, 60386 Frankfurt am Main, GermanyDue to the increasing pharmaceutical interest of oligonucleotides, for example in antisense therapy and vaccines, their analytical characterization is of fundamental importance due to their complex structure. For this purpose, mass spectrometry is a viable tool for structural studies of nucleic acids. Structural information regarding the primary sequence of a nucleic acid can reliably be gained via tandem mass spectrometry (MSMS) fragmentation. In this work, we present the characteristic fragmentation behavior of short-chain oligonucleotides (15–35 nucleotides) with respect to the collision-induced dissociation (CID) voltage used. The relationship and influence of the length of the oligonucleotide and its charge state is also discussed. The results presented here can be helpful for estimating the required fragmentation energies of short-chain oligonucleotides and their sequencing.https://www.mdpi.com/1420-3049/28/3/1169oligonucleotidessequence confirmationfragment ionscharge statecollision energy |
| spellingShingle | Christopher Gawlig Michael Rühl Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry Molecules oligonucleotides sequence confirmation fragment ions charge state collision energy |
| title | Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry |
| title_full | Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry |
| title_fullStr | Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry |
| title_full_unstemmed | Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry |
| title_short | Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry |
| title_sort | investigation of the influence of charge state and collision energy on oligonucleotide fragmentation by tandem mass spectrometry |
| topic | oligonucleotides sequence confirmation fragment ions charge state collision energy |
| url | https://www.mdpi.com/1420-3049/28/3/1169 |
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