Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide
A simple, precise and sensitive indirect spectrophotometric method is described for the assay of Paracetamol in its pure form and pharmaceutical formulations in the aqueous medium. The method is based on the oxidation of the Paracetamol with an excess of N-Bromosuccinimide (NBS) in alkaline medium a...
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| Format: | Article |
| Language: | Arabic |
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College of Education for Pure Sciences
2020-09-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_164827_f3f6bf139f2551d9de6c1ba38670b81e.pdf |
| _version_ | 1818409117488775168 |
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| author | Theiaa Al-Sabha Zahraa Al-Gubouri |
| author_facet | Theiaa Al-Sabha Zahraa Al-Gubouri |
| author_sort | Theiaa Al-Sabha |
| collection | DOAJ |
| description | A simple, precise and sensitive indirect spectrophotometric method is described for the assay of Paracetamol in its pure form and pharmaceutical formulations in the aqueous medium. The method is based on the oxidation of the Paracetamol with an excess of N-Bromosuccinimide (NBS) in alkaline medium and the residual oxidizing agent bleaches the purple-colored Eriochrom black-T (EBT) to colorless species which is measured at 516 nm at room temperature. Calibration graph is linear over 0.5-9 µg mL-1 and molar absorptivity is 3.7×104 L.mol-1.cm-1. The detection and quantification limits were 0.068 and 0.229 µgmL-1 respectively. The accuracy (Average recovery %) is 98.57, and Precision (RSD) is ≤ 1.5. No interference effect has been observed from the excipients that exist in drug formulations. The method has been applied successfully in the determination of the Paracetamol in its commercial formulations (injection, syrup, and tablet), and compared favorably with other spectrophotometric methods used different reagents. The reaction mechanism for the oxidation of Paracetamol and EBT was postulated. |
| first_indexed | 2024-12-14T09:54:32Z |
| format | Article |
| id | doaj.art-831c6fb702b44d1eb4150cdf8107d801 |
| institution | Directory Open Access Journal |
| issn | 1812-125X 2664-2530 |
| language | Arabic |
| last_indexed | 2024-12-14T09:54:32Z |
| publishDate | 2020-09-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | مجلة التربية والعلم |
| spelling | doaj.art-831c6fb702b44d1eb4150cdf8107d8012022-12-21T23:07:27ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302020-09-0129324625610.33899/edusj.2020.126846.1060164827Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-BromosuccinimideTheiaa Al-Sabha0Zahraa Al-Gubouri1Department of Chemistry, College of Education for Pure Science, University of MosulChemistry department, College of Education for pure science, University of Mosul, Mosul, IraqA simple, precise and sensitive indirect spectrophotometric method is described for the assay of Paracetamol in its pure form and pharmaceutical formulations in the aqueous medium. The method is based on the oxidation of the Paracetamol with an excess of N-Bromosuccinimide (NBS) in alkaline medium and the residual oxidizing agent bleaches the purple-colored Eriochrom black-T (EBT) to colorless species which is measured at 516 nm at room temperature. Calibration graph is linear over 0.5-9 µg mL-1 and molar absorptivity is 3.7×104 L.mol-1.cm-1. The detection and quantification limits were 0.068 and 0.229 µgmL-1 respectively. The accuracy (Average recovery %) is 98.57, and Precision (RSD) is ≤ 1.5. No interference effect has been observed from the excipients that exist in drug formulations. The method has been applied successfully in the determination of the Paracetamol in its commercial formulations (injection, syrup, and tablet), and compared favorably with other spectrophotometric methods used different reagents. The reaction mechanism for the oxidation of Paracetamol and EBT was postulated.https://edusj.mosuljournals.com/article_164827_f3f6bf139f2551d9de6c1ba38670b81e.pdfspectrophotometry,,,،,؛ebt,,,،,؛paracetamol,,,،,؛oxidation,,,،,؛nbs |
| spellingShingle | Theiaa Al-Sabha Zahraa Al-Gubouri Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide مجلة التربية والعلم spectrophotometry,, ,،,؛ebt,, ,،,؛paracetamol,, ,،,؛oxidation,, ,،,؛nbs |
| title | Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide |
| title_full | Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide |
| title_fullStr | Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide |
| title_full_unstemmed | Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide |
| title_short | Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide |
| title_sort | indirect spectrophotometric determination of paracetamol via decolorization of eriochrom black t with n bromosuccinimide |
| topic | spectrophotometry,, ,،,؛ebt,, ,،,؛paracetamol,, ,،,؛oxidation,, ,،,؛nbs |
| url | https://edusj.mosuljournals.com/article_164827_f3f6bf139f2551d9de6c1ba38670b81e.pdf |
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