Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles
Silver diamine fluoride (SDF) is an outstanding dental material for arresting and preventing caries, but some drawbacks, such as high flowability due to low viscosity and cytotoxicity to the pulp, have been reported. To overcome these problems, copper-doped bioactive glass nanoparticles (CuBGns) wer...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/6/1137 |
| _version_ | 1827657558749347840 |
|---|---|
| author | Se-Jung Bang Soo-Kyung Jun Yu-Jin Kim Jun-Yong Ahn Huong Thu Vu Nandin Mandakhbayar Mi-Ran Han Jun-Haeng Lee Jong-Bin Kim Jong-Soo Kim Jonathan C. Knowles Hye-Sung Kim Hae-Hyoung Lee Ji-Sun Shin Jung-Hwan Lee |
| author_facet | Se-Jung Bang Soo-Kyung Jun Yu-Jin Kim Jun-Yong Ahn Huong Thu Vu Nandin Mandakhbayar Mi-Ran Han Jun-Haeng Lee Jong-Bin Kim Jong-Soo Kim Jonathan C. Knowles Hye-Sung Kim Hae-Hyoung Lee Ji-Sun Shin Jung-Hwan Lee |
| author_sort | Se-Jung Bang |
| collection | DOAJ |
| description | Silver diamine fluoride (SDF) is an outstanding dental material for arresting and preventing caries, but some drawbacks, such as high flowability due to low viscosity and cytotoxicity to the pulp, have been reported. To overcome these problems, copper-doped bioactive glass nanoparticles (CuBGns) were combined with SDF. After synthesis, CuBGns were examined by physical analysis and added in SDF at different weight/volume% (SDF@CuBGn). After assessing physical properties (viscosity and flowability) of SDF@CuBGn, physicochemical properties (morphology before and after simulated body fluid (SBF) immersion and ion release) of SDF@CuBGn-applied hydroxyapatite (HA) discs were evaluated. Biological properties were further evaluated by cytotoxicity test to pulp stem cells and antibacterial effect on cariogenic organisms (<i>Streptococcus mutans</i> and <i>Staphylococcus aureus</i>). Combining CuBGns in SDF increased the viscosity up to 3 times while lowering the flowability. More CuBGns and functional elements in SDF (Ag and F) were deposited on the HA substrate, even after SBF immersion test for 14 days, and they showed higher Cu, Ca, and Si release without changing F and Ag release. Cell viability test suggested lower cytotoxicity in SDF@CuBGn-applied HA, while CuBGns in SDF boosted antibacterial effect against <i>S. aureus</i>, ~27% in diameter of agar diffusion test. In conclusion, the addition of CuBGn to SDF enhances viscosity, Ag and F deposition, and antibacterial effects while reducing cell toxicity, highlighting the role of bioactive CuBGns for regulating physical and biological effects of dental materials. |
| first_indexed | 2024-03-09T22:46:32Z |
| format | Article |
| id | doaj.art-831eca0ccf0f4f7b9c8a776ece213d29 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:46:32Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-831eca0ccf0f4f7b9c8a776ece213d292023-11-23T18:28:49ZengMDPI AGPharmaceutics1999-49232022-05-01146113710.3390/pharmaceutics14061137Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass NanoparticlesSe-Jung Bang0Soo-Kyung Jun1Yu-Jin Kim2Jun-Yong Ahn3Huong Thu Vu4Nandin Mandakhbayar5Mi-Ran Han6Jun-Haeng Lee7Jong-Bin Kim8Jong-Soo Kim9Jonathan C. Knowles10Hye-Sung Kim11Hae-Hyoung Lee12Ji-Sun Shin13Jung-Hwan Lee14Department of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaInstitute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, KoreaInstitute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, KoreaDepartment of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Pediatric Dentistry, College of Dentistry, Dankook University, Cheonan 31116, KoreaDepartment of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, KoreaSilver diamine fluoride (SDF) is an outstanding dental material for arresting and preventing caries, but some drawbacks, such as high flowability due to low viscosity and cytotoxicity to the pulp, have been reported. To overcome these problems, copper-doped bioactive glass nanoparticles (CuBGns) were combined with SDF. After synthesis, CuBGns were examined by physical analysis and added in SDF at different weight/volume% (SDF@CuBGn). After assessing physical properties (viscosity and flowability) of SDF@CuBGn, physicochemical properties (morphology before and after simulated body fluid (SBF) immersion and ion release) of SDF@CuBGn-applied hydroxyapatite (HA) discs were evaluated. Biological properties were further evaluated by cytotoxicity test to pulp stem cells and antibacterial effect on cariogenic organisms (<i>Streptococcus mutans</i> and <i>Staphylococcus aureus</i>). Combining CuBGns in SDF increased the viscosity up to 3 times while lowering the flowability. More CuBGns and functional elements in SDF (Ag and F) were deposited on the HA substrate, even after SBF immersion test for 14 days, and they showed higher Cu, Ca, and Si release without changing F and Ag release. Cell viability test suggested lower cytotoxicity in SDF@CuBGn-applied HA, while CuBGns in SDF boosted antibacterial effect against <i>S. aureus</i>, ~27% in diameter of agar diffusion test. In conclusion, the addition of CuBGn to SDF enhances viscosity, Ag and F deposition, and antibacterial effects while reducing cell toxicity, highlighting the role of bioactive CuBGns for regulating physical and biological effects of dental materials.https://www.mdpi.com/1999-4923/14/6/1137silver diamine fluoridecopper-doped bioactive glass nanoparticlehydroxyapatite discviscosityphysicochemical propertiesbiological properties |
| spellingShingle | Se-Jung Bang Soo-Kyung Jun Yu-Jin Kim Jun-Yong Ahn Huong Thu Vu Nandin Mandakhbayar Mi-Ran Han Jun-Haeng Lee Jong-Bin Kim Jong-Soo Kim Jonathan C. Knowles Hye-Sung Kim Hae-Hyoung Lee Ji-Sun Shin Jung-Hwan Lee Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles Pharmaceutics silver diamine fluoride copper-doped bioactive glass nanoparticle hydroxyapatite disc viscosity physicochemical properties biological properties |
| title | Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles |
| title_full | Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles |
| title_fullStr | Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles |
| title_full_unstemmed | Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles |
| title_short | Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles |
| title_sort | characterization of physical and biological properties of a caries arresting liquid containing copper doped bioglass nanoparticles |
| topic | silver diamine fluoride copper-doped bioactive glass nanoparticle hydroxyapatite disc viscosity physicochemical properties biological properties |
| url | https://www.mdpi.com/1999-4923/14/6/1137 |
| work_keys_str_mv | AT sejungbang characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT sookyungjun characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT yujinkim characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT junyongahn characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT huongthuvu characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT nandinmandakhbayar characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT miranhan characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT junhaenglee characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT jongbinkim characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT jongsookim characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT jonathancknowles characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT hyesungkim characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT haehyounglee characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT jisunshin characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles AT junghwanlee characterizationofphysicalandbiologicalpropertiesofacariesarrestingliquidcontainingcopperdopedbioglassnanoparticles |