Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612100427X |
| _version_ | 1818461916540960768 |
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| author | Elena Romano Piera Trionfini Roberta Giampietro Ariela Benigni Susanna Tomasoni |
| author_facet | Elena Romano Piera Trionfini Roberta Giampietro Ariela Benigni Susanna Tomasoni |
| author_sort | Elena Romano |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed.In this study, we used CRISPR-Cas9 technology to generate an isogenic iPSC line with a homozygous frameshift mutation in the MHC II transactivator (CIITA) gene. The CIITA-/- iPSCs exhibit typical morphology of pluripotent cells, normal karyotype, expression of pluripotency markers and differentiation capacity in the three germ layers. |
| first_indexed | 2024-12-14T23:53:45Z |
| format | Article |
| id | doaj.art-831fefabf399488f847c57f65799c790 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-14T23:53:45Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-831fefabf399488f847c57f65799c7902022-12-21T22:43:10ZengElsevierStem Cell Research1873-50612021-12-0157102580Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 systemElena Romano0Piera Trionfini1Roberta Giampietro2Ariela Benigni3Susanna Tomasoni4Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, ItalyCorresponding authors.; Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, ItalyIstituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, ItalyIstituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, ItalyCorresponding authors.; Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, ItalyHuman induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed.In this study, we used CRISPR-Cas9 technology to generate an isogenic iPSC line with a homozygous frameshift mutation in the MHC II transactivator (CIITA) gene. The CIITA-/- iPSCs exhibit typical morphology of pluripotent cells, normal karyotype, expression of pluripotency markers and differentiation capacity in the three germ layers.http://www.sciencedirect.com/science/article/pii/S187350612100427X |
| spellingShingle | Elena Romano Piera Trionfini Roberta Giampietro Ariela Benigni Susanna Tomasoni Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system Stem Cell Research |
| title | Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system |
| title_full | Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system |
| title_fullStr | Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system |
| title_full_unstemmed | Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system |
| title_short | Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system |
| title_sort | generation of a homozygous ciita knockout ips cell line using the crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S187350612100427X |
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