The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum
DDT (1,1,1-trichloro-2,2 bis(4-chlorophenyl) ethane) is a synthetic insecticide that has several negative effects on the environment and humans. Therefore, determining an effective method to reduce DDT may give a beneficial impact. Brown-rot fungus, Gloeophyllum trabeum, is well known to have the ab...
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Elsevier
2023-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023054245 |
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| author | Hamdan Dwi Rizqi Adi Setyo Purnomo Aulia Ulfi |
| author_facet | Hamdan Dwi Rizqi Adi Setyo Purnomo Aulia Ulfi |
| author_sort | Hamdan Dwi Rizqi |
| collection | DOAJ |
| description | DDT (1,1,1-trichloro-2,2 bis(4-chlorophenyl) ethane) is a synthetic insecticide that has several negative effects on the environment and humans. Therefore, determining an effective method to reduce DDT may give a beneficial impact. Brown-rot fungus, Gloeophyllum trabeum, is well known to have the ability to degrade DDT, even though it might require long-term remediation. In this study, the effect of the addition of bacteria on the biodegradation of DDT by G. trabeum had been investigated. Bacillus subtilis, Pseudomonas aeruginosa, and Ralstonia pickettii were screened for the bacteria which the volume of bacteria at 1, 3, 5, and 10 mL and the time range of addition of bacteria on days 0, 1, 3, and 5. The addition of B. subtilis, P. aeruginosa, and R. pickettii bacteria into the G. trabeum culture increased DDT biodegradation to approximately 62.02; 74.66; and 75.72%, respectively, in which G. trabeum was only able to degrade DDT by 54.52% for 7 days of incubation. R. pickettii enhanced the degradation process, in which the addition of 10 mL of this bacterium at day 1 possessed the highest value of 92.41% within 7 days of incubation. DDD was detected to be a product metabolite through a dechlorination reaction. This study indicated that mixed cultures of G. trabeum and R. pickettii can be used to degrade DDT. |
| first_indexed | 2024-03-12T21:36:33Z |
| format | Article |
| id | doaj.art-832320935a3844cba9b0c9c4d3f96da6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:36:33Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-832320935a3844cba9b0c9c4d3f96da62023-07-27T05:58:56ZengElsevierHeliyon2405-84402023-07-0197e18216The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeumHamdan Dwi Rizqi0Adi Setyo Purnomo1Aulia Ulfi2Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, IndonesiaCorresponding author.; Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, IndonesiaDepartment of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, IndonesiaDDT (1,1,1-trichloro-2,2 bis(4-chlorophenyl) ethane) is a synthetic insecticide that has several negative effects on the environment and humans. Therefore, determining an effective method to reduce DDT may give a beneficial impact. Brown-rot fungus, Gloeophyllum trabeum, is well known to have the ability to degrade DDT, even though it might require long-term remediation. In this study, the effect of the addition of bacteria on the biodegradation of DDT by G. trabeum had been investigated. Bacillus subtilis, Pseudomonas aeruginosa, and Ralstonia pickettii were screened for the bacteria which the volume of bacteria at 1, 3, 5, and 10 mL and the time range of addition of bacteria on days 0, 1, 3, and 5. The addition of B. subtilis, P. aeruginosa, and R. pickettii bacteria into the G. trabeum culture increased DDT biodegradation to approximately 62.02; 74.66; and 75.72%, respectively, in which G. trabeum was only able to degrade DDT by 54.52% for 7 days of incubation. R. pickettii enhanced the degradation process, in which the addition of 10 mL of this bacterium at day 1 possessed the highest value of 92.41% within 7 days of incubation. DDD was detected to be a product metabolite through a dechlorination reaction. This study indicated that mixed cultures of G. trabeum and R. pickettii can be used to degrade DDT.http://www.sciencedirect.com/science/article/pii/S2405844023054245BiodegradationDDTBacillus subtilisPseudomonas aeruginosaRalstonia pickettiiGloeophyllum trabeum |
| spellingShingle | Hamdan Dwi Rizqi Adi Setyo Purnomo Aulia Ulfi The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum Heliyon Biodegradation DDT Bacillus subtilis Pseudomonas aeruginosa Ralstonia pickettii Gloeophyllum trabeum |
| title | The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum |
| title_full | The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum |
| title_fullStr | The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum |
| title_full_unstemmed | The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum |
| title_short | The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum |
| title_sort | effect of bacteria addition on ddt biodegradation by brown rot fungus gloeophyllum trabeum |
| topic | Biodegradation DDT Bacillus subtilis Pseudomonas aeruginosa Ralstonia pickettii Gloeophyllum trabeum |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023054245 |
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