Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties
The present study aimed to isolate actinomycetes from soil samples collected from Western Ghats regions, Tamil Nadu, India. Totally 400 actinomycetes isolates were isolated, of which 161 (40.2%) showed antimicrobial activity in preliminary screening. Secondary screening yielded 27 active isolates; t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2017-06-01
|
| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853516301494 |
| _version_ | 1828380937545580544 |
|---|---|
| author | Ganesan Pathalam Host Antony David Rajendran Daniel Reegan Appadurai Munusamy Rajiv Gandhi Gabriel Paulraj Michael Ignacimuthu Savarimuthu Abdullah Al-Dhabi Naif |
| author_facet | Ganesan Pathalam Host Antony David Rajendran Daniel Reegan Appadurai Munusamy Rajiv Gandhi Gabriel Paulraj Michael Ignacimuthu Savarimuthu Abdullah Al-Dhabi Naif |
| author_sort | Ganesan Pathalam |
| collection | DOAJ |
| description | The present study aimed to isolate actinomycetes from soil samples collected from Western Ghats regions, Tamil Nadu, India. Totally 400 actinomycetes isolates were isolated, of which 161 (40.2%) showed antimicrobial activity in preliminary screening. Secondary screening yielded 27 active isolates; twelve of them showed good antimicrobial and larvicidal activities. Five isolates namely S12-4, S12-17, S12-8, DPS-14 and FMS-20 presented 98%, 93%, 87%, 82%, and 80% larvicidal activity against Cx. quinquefasciatus; very low larvicidal activity was observed against Ae. aegypti. S12-4 showed 100% larval mortality against An. stephensi. RAPD results showed highest similarity between DPS-122 and KFS-24 (0.89246). The polymorphic index observed in the primers ranged from 0.286 (OPA-09) to 0.07 (OPC-18). The results suggest that Western Ghats regions may be a good natural source for isolation of actinobacteria and thereby for discovering novel molecules with antimicrobial and mosquito larvicidal properties. |
| first_indexed | 2024-12-10T04:06:06Z |
| format | Article |
| id | doaj.art-832491f6c37142c28b6a41f05eeec0ef |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-10T04:06:06Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-832491f6c37142c28b6a41f05eeec0ef2022-12-22T02:02:50ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352017-06-016220921710.1016/j.bjbas.2017.04.002Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal propertiesGanesan Pathalam0Host Antony David Rajendran1Daniel Reegan Appadurai2Munusamy Rajiv Gandhi3Gabriel Paulraj Michael4Ignacimuthu Savarimuthu5Abdullah Al-Dhabi Naif6Entomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaEntomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaEntomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaEntomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaEntomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaEntomology Research Institute, Loyola College, Chennai 600 034, Tamil Nadu, IndiaAddiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaThe present study aimed to isolate actinomycetes from soil samples collected from Western Ghats regions, Tamil Nadu, India. Totally 400 actinomycetes isolates were isolated, of which 161 (40.2%) showed antimicrobial activity in preliminary screening. Secondary screening yielded 27 active isolates; twelve of them showed good antimicrobial and larvicidal activities. Five isolates namely S12-4, S12-17, S12-8, DPS-14 and FMS-20 presented 98%, 93%, 87%, 82%, and 80% larvicidal activity against Cx. quinquefasciatus; very low larvicidal activity was observed against Ae. aegypti. S12-4 showed 100% larval mortality against An. stephensi. RAPD results showed highest similarity between DPS-122 and KFS-24 (0.89246). The polymorphic index observed in the primers ranged from 0.286 (OPA-09) to 0.07 (OPC-18). The results suggest that Western Ghats regions may be a good natural source for isolation of actinobacteria and thereby for discovering novel molecules with antimicrobial and mosquito larvicidal properties.http://www.sciencedirect.com/science/article/pii/S2314853516301494Antimicrobial activityLarvicidal bioassayRAPDCulex quinquefasciatusAnopheles stephensiAedes aegypti |
| spellingShingle | Ganesan Pathalam Host Antony David Rajendran Daniel Reegan Appadurai Munusamy Rajiv Gandhi Gabriel Paulraj Michael Ignacimuthu Savarimuthu Abdullah Al-Dhabi Naif Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties Beni-Suef University Journal of Basic and Applied Sciences Antimicrobial activity Larvicidal bioassay RAPD Culex quinquefasciatus Anopheles stephensi Aedes aegypti |
| title | Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| title_full | Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| title_fullStr | Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| title_full_unstemmed | Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| title_short | Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| title_sort | isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties |
| topic | Antimicrobial activity Larvicidal bioassay RAPD Culex quinquefasciatus Anopheles stephensi Aedes aegypti |
| url | http://www.sciencedirect.com/science/article/pii/S2314853516301494 |
| work_keys_str_mv | AT ganesanpathalam isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT hostantonydavidrajendran isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT danielreeganappadurai isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT munusamyrajivgandhi isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT gabrielpaulrajmichael isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT ignacimuthusavarimuthu isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties AT abdullahaldhabinaif isolationandmolecularcharacterizationofactinomyceteswithantimicrobialandmosquitolarvicidalproperties |