Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery
Abstract Regulatory arrest peptides interact with specific residues on bacterial ribosomes and arrest their own translation. Here, we analyse over 30,000 bacterial genome sequences to identify additional Sec/YidC-related arrest peptides, followed by in vivo and in vitro analyses. We find that Sec/Yi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46993-3 |
| _version_ | 1797219744390053888 |
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| author | Keigo Fujiwara Naoko Tsuji Mayu Yoshida Hiraku Takada Shinobu Chiba |
| author_facet | Keigo Fujiwara Naoko Tsuji Mayu Yoshida Hiraku Takada Shinobu Chiba |
| author_sort | Keigo Fujiwara |
| collection | DOAJ |
| description | Abstract Regulatory arrest peptides interact with specific residues on bacterial ribosomes and arrest their own translation. Here, we analyse over 30,000 bacterial genome sequences to identify additional Sec/YidC-related arrest peptides, followed by in vivo and in vitro analyses. We find that Sec/YidC-related arrest peptides show patchy, but widespread, phylogenetic distribution throughout the bacterial domain. Several of the identified peptides contain distinct conserved sequences near the C-termini, but are still able to efficiently stall bacterial ribosomes in vitro and in vivo. In addition, we identify many arrest peptides that share an R-A-P-P-like sequence, suggesting that this sequence might serve as a common evolutionary seed to overcome ribosomal structural differences across species. |
| first_indexed | 2024-04-24T12:38:31Z |
| format | Article |
| id | doaj.art-8325ba1e4e4d4765bbb7a5e7b7c41704 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T12:38:31Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-8325ba1e4e4d4765bbb7a5e7b7c417042024-04-07T11:24:02ZengNature PortfolioNature Communications2041-17232024-04-0115111610.1038/s41467-024-46993-3Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machineryKeigo Fujiwara0Naoko Tsuji1Mayu Yoshida2Hiraku Takada3Shinobu Chiba4Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-KuFaculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-KuFaculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-KuFaculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-KuFaculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-KuAbstract Regulatory arrest peptides interact with specific residues on bacterial ribosomes and arrest their own translation. Here, we analyse over 30,000 bacterial genome sequences to identify additional Sec/YidC-related arrest peptides, followed by in vivo and in vitro analyses. We find that Sec/YidC-related arrest peptides show patchy, but widespread, phylogenetic distribution throughout the bacterial domain. Several of the identified peptides contain distinct conserved sequences near the C-termini, but are still able to efficiently stall bacterial ribosomes in vitro and in vivo. In addition, we identify many arrest peptides that share an R-A-P-P-like sequence, suggesting that this sequence might serve as a common evolutionary seed to overcome ribosomal structural differences across species.https://doi.org/10.1038/s41467-024-46993-3 |
| spellingShingle | Keigo Fujiwara Naoko Tsuji Mayu Yoshida Hiraku Takada Shinobu Chiba Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery Nature Communications |
| title | Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| title_full | Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| title_fullStr | Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| title_full_unstemmed | Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| title_short | Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| title_sort | patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery |
| url | https://doi.org/10.1038/s41467-024-46993-3 |
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