Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System.
In this study, the metabolic and physiological potential evaluator system based on Kyoto Encyclopedia of Genes and Genomes (KEGG) functional modules was employed to establish a functional classification of archaeal species and to determine the comprehensive functions (functionome) of the previously...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4510362?pdf=render |
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| author | Hideto Takami Wataru Arai Kazuhiro Takemoto Ikuo Uchiyama Takeaki Taniguchi |
| author_facet | Hideto Takami Wataru Arai Kazuhiro Takemoto Ikuo Uchiyama Takeaki Taniguchi |
| author_sort | Hideto Takami |
| collection | DOAJ |
| description | In this study, the metabolic and physiological potential evaluator system based on Kyoto Encyclopedia of Genes and Genomes (KEGG) functional modules was employed to establish a functional classification of archaeal species and to determine the comprehensive functions (functionome) of the previously uncultivated thermophile "Candidatus Caldiarchaeum subterraneum" (Ca. C. subterraneum). A phylogenetic analysis based on the concatenated sequences of proteins common among 142 archaea and 2 bacteria, and among 137 archaea and 13 unicellular eukaryotes suggested that Ca. C. subterraneum is closely related to thaumarchaeotic species. Consistent with the results of the phylogenetic analysis, clustering and principal component analyses based on the completion ratio patterns for all KEGG modules in 79 archaeal species suggested that the overall metabolic and physiological potential of Ca. C. subterraneum is similar to that of thaumarchaeotic species. However, Ca. C. subterraneum possessed almost no genes in the modules required for nitrification and the hydroxypropionate-hydroxybutyrate cycle for carbon fixation, unlike thaumarchaeotic species. However, it possessed all genes in the modules required for central carbohydrate metabolism, such as glycolysis, pyruvate oxidation, the tricarboxylic acid (TCA) cycle, and the glyoxylate cycle, as well as multiple sets of sugar and branched chain amino acid ABC transporters. These metabolic and physiological features appear to support the predominantly aerobic character of Ca. C. subterraneum, which lives in a subsurface thermophilic microbial mat community with a heterotrophic lifestyle. |
| first_indexed | 2024-12-11T23:50:13Z |
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| id | doaj.art-83264928ad84473f97b72b14b6c337ed |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T23:50:13Z |
| publishDate | 2015-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-83264928ad84473f97b72b14b6c337ed2022-12-22T00:45:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013299410.1371/journal.pone.0132994Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System.Hideto TakamiWataru AraiKazuhiro TakemotoIkuo UchiyamaTakeaki TaniguchiIn this study, the metabolic and physiological potential evaluator system based on Kyoto Encyclopedia of Genes and Genomes (KEGG) functional modules was employed to establish a functional classification of archaeal species and to determine the comprehensive functions (functionome) of the previously uncultivated thermophile "Candidatus Caldiarchaeum subterraneum" (Ca. C. subterraneum). A phylogenetic analysis based on the concatenated sequences of proteins common among 142 archaea and 2 bacteria, and among 137 archaea and 13 unicellular eukaryotes suggested that Ca. C. subterraneum is closely related to thaumarchaeotic species. Consistent with the results of the phylogenetic analysis, clustering and principal component analyses based on the completion ratio patterns for all KEGG modules in 79 archaeal species suggested that the overall metabolic and physiological potential of Ca. C. subterraneum is similar to that of thaumarchaeotic species. However, Ca. C. subterraneum possessed almost no genes in the modules required for nitrification and the hydroxypropionate-hydroxybutyrate cycle for carbon fixation, unlike thaumarchaeotic species. However, it possessed all genes in the modules required for central carbohydrate metabolism, such as glycolysis, pyruvate oxidation, the tricarboxylic acid (TCA) cycle, and the glyoxylate cycle, as well as multiple sets of sugar and branched chain amino acid ABC transporters. These metabolic and physiological features appear to support the predominantly aerobic character of Ca. C. subterraneum, which lives in a subsurface thermophilic microbial mat community with a heterotrophic lifestyle.http://europepmc.org/articles/PMC4510362?pdf=render |
| spellingShingle | Hideto Takami Wataru Arai Kazuhiro Takemoto Ikuo Uchiyama Takeaki Taniguchi Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. PLoS ONE |
| title | Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. |
| title_full | Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. |
| title_fullStr | Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. |
| title_full_unstemmed | Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. |
| title_short | Functional Classification of Uncultured "Candidatus Caldiarchaeum subterraneum" Using the Maple System. |
| title_sort | functional classification of uncultured candidatus caldiarchaeum subterraneum using the maple system |
| url | http://europepmc.org/articles/PMC4510362?pdf=render |
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