Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy
<i>Salvia mltiorrhiza</i> Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/9/2043 |
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| author | Yuanyuan Li Zhuoni Hou Feng Su Jipeng Chen Xiaodan Zhang Ling Xu Dongfeng Yang Zongsuo Liang |
| author_facet | Yuanyuan Li Zhuoni Hou Feng Su Jipeng Chen Xiaodan Zhang Ling Xu Dongfeng Yang Zongsuo Liang |
| author_sort | Yuanyuan Li |
| collection | DOAJ |
| description | <i>Salvia mltiorrhiza</i> Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB root has not been verified so far. The purpose of this study was to establish a fast and simple method to quantify the tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh <i>Salvia Miltiorrhiza</i> Bunge root without any pre-purification steps using <sup>1</sup>H-NMR spectroscopy. The process is as follows: first, 70% methanol aqueous extracts of fresh <i>Salvia Miltiorrhiza</i> Bunge roots were quantitatively analyzed for tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone using <sup>1</sup>H-NMR spectroscopy. Different internal standards were tested and the validated method was compared with HPLC. 3,4,5-trichloropyridine was chosen as the internal standard. Twelve samples of <i>Salvia Miltiorrhiza</i> Bunge were quantitatively analyzed by qNMR and HPLC respectively. Then, the results were analyzed by chemometric approaches. This NMR method offers a fast, stable, and accurate analysis of four ketones: tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh roots of <i>Salvia Miltiorrhiza</i> Bunge. |
| first_indexed | 2024-03-10T20:11:33Z |
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| language | English |
| last_indexed | 2024-03-10T20:11:33Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-83285b597b684e7d9eb6841cc670c46a2023-11-19T22:53:56ZengMDPI AGMolecules1420-30492020-04-01259204310.3390/molecules25092043Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance SpectroscopyYuanyuan Li0Zhuoni Hou1Feng Su2Jipeng Chen3Xiaodan Zhang4Ling Xu5Dongfeng Yang6Zongsuo Liang7The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaThe Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaKey Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 18 Chao Wang Road, Hangzhou 310014, ChinaKey Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 18 Chao Wang Road, Hangzhou 310014, ChinaThe Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaThe Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaThe Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaThe Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China<i>Salvia mltiorrhiza</i> Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB root has not been verified so far. The purpose of this study was to establish a fast and simple method to quantify the tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh <i>Salvia Miltiorrhiza</i> Bunge root without any pre-purification steps using <sup>1</sup>H-NMR spectroscopy. The process is as follows: first, 70% methanol aqueous extracts of fresh <i>Salvia Miltiorrhiza</i> Bunge roots were quantitatively analyzed for tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone using <sup>1</sup>H-NMR spectroscopy. Different internal standards were tested and the validated method was compared with HPLC. 3,4,5-trichloropyridine was chosen as the internal standard. Twelve samples of <i>Salvia Miltiorrhiza</i> Bunge were quantitatively analyzed by qNMR and HPLC respectively. Then, the results were analyzed by chemometric approaches. This NMR method offers a fast, stable, and accurate analysis of four ketones: tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh roots of <i>Salvia Miltiorrhiza</i> Bunge.https://www.mdpi.com/1420-3049/25/9/2043qNMR<i>Salvia Miltiorrhiza</i> Bungetanshinone Itanshinone IIAdihydrotanshinonecryptotanshinone |
| spellingShingle | Yuanyuan Li Zhuoni Hou Feng Su Jipeng Chen Xiaodan Zhang Ling Xu Dongfeng Yang Zongsuo Liang Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy Molecules qNMR <i>Salvia Miltiorrhiza</i> Bunge tanshinone I tanshinone IIA dihydrotanshinone cryptotanshinone |
| title | Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy |
| title_full | Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy |
| title_fullStr | Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy |
| title_full_unstemmed | Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy |
| title_short | Quantitative Determination and Validation of Four Ketones in <i>Salvia miltiorrhiza</i> Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy |
| title_sort | quantitative determination and validation of four ketones in i salvia miltiorrhiza i bunge using quantitative proton nuclear magnetic resonance spectroscopy |
| topic | qNMR <i>Salvia Miltiorrhiza</i> Bunge tanshinone I tanshinone IIA dihydrotanshinone cryptotanshinone |
| url | https://www.mdpi.com/1420-3049/25/9/2043 |
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