Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color
The unicellular microalgae <i>Haematococcus lacustris</i> is an astaxanthin-rich organism that is widely used for commercial cultivation, but its main limitation is its relatively low biomass yield. It is widely accepted that the use of appropriate high light intensity could promote alga...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/7/839 |
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| author | Lu Liu Kezhen Ying Kebi Wu Si Tang Jin Zhou Zhonghua Cai |
| author_facet | Lu Liu Kezhen Ying Kebi Wu Si Tang Jin Zhou Zhonghua Cai |
| author_sort | Lu Liu |
| collection | DOAJ |
| description | The unicellular microalgae <i>Haematococcus lacustris</i> is an astaxanthin-rich organism that is widely used for commercial cultivation, but its main limitation is its relatively low biomass yield. It is widely accepted that the use of appropriate high light intensity could promote algal growth; however, <i>H. lacustris</i> is very sensitive to high-intensity light, and its growth can be readily arrested by inappropriate illumination. To exploit the growth-promoting benefit of higher light intensities while avoiding growth arrestment, we examined the growth of <i>H. lacustris</i> under high light intensities using various light profiles, including different light colors and light/dark cycles. The results show that light color treatments could not alleviate cellular stress under high light intensities; however, it was interesting to find that red light was favored the most by cells out of all the colors. In terms of the light/dark cycle, the 2/2 h light/dark cycle treatment was shown to lead to the highest specific growth rate, which was 46% higher than that achieved in the control treatment (18 μmol/m<sup>2</sup>·s light intensity, white light). Therefore, in further experiments, the 2/2 h light/dark cycle with red-light treatment was examined. The results show that this combination enabled a significantly higher specific growth rate, which was 66.5% higher than that achieved in the control treatment (18 μmol/m<sup>2</sup>·s light intensity, white light). These findings offer new strategies for the optimization of illumination for the growth of <i>H. lacustris</i> and lay the foundations for more reasonable lighting utilization for the cultivation of commercially valuable algal species. |
| first_indexed | 2024-03-09T03:19:00Z |
| format | Article |
| id | doaj.art-832b58d5df0142f49b9035dbe8a8182f |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-09T03:19:00Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-832b58d5df0142f49b9035dbe8a8182f2023-12-03T15:14:29ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-06-0110783910.3390/jmse10070839Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light ColorLu Liu0Kezhen Ying1Kebi Wu2Si Tang3Jin Zhou4Zhonghua Cai5School of Life Sciences, Tsinghua University, Beijing 100086, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaSchool of Life Sciences, Tsinghua University, Beijing 100086, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, ChinaSchool of Life Sciences, Tsinghua University, Beijing 100086, ChinaThe unicellular microalgae <i>Haematococcus lacustris</i> is an astaxanthin-rich organism that is widely used for commercial cultivation, but its main limitation is its relatively low biomass yield. It is widely accepted that the use of appropriate high light intensity could promote algal growth; however, <i>H. lacustris</i> is very sensitive to high-intensity light, and its growth can be readily arrested by inappropriate illumination. To exploit the growth-promoting benefit of higher light intensities while avoiding growth arrestment, we examined the growth of <i>H. lacustris</i> under high light intensities using various light profiles, including different light colors and light/dark cycles. The results show that light color treatments could not alleviate cellular stress under high light intensities; however, it was interesting to find that red light was favored the most by cells out of all the colors. In terms of the light/dark cycle, the 2/2 h light/dark cycle treatment was shown to lead to the highest specific growth rate, which was 46% higher than that achieved in the control treatment (18 μmol/m<sup>2</sup>·s light intensity, white light). Therefore, in further experiments, the 2/2 h light/dark cycle with red-light treatment was examined. The results show that this combination enabled a significantly higher specific growth rate, which was 66.5% higher than that achieved in the control treatment (18 μmol/m<sup>2</sup>·s light intensity, white light). These findings offer new strategies for the optimization of illumination for the growth of <i>H. lacustris</i> and lay the foundations for more reasonable lighting utilization for the cultivation of commercially valuable algal species.https://www.mdpi.com/2077-1312/10/7/839<i>Haematococcus lacustris</i>light colorlight/dark cyclelight colorhigh light intensity |
| spellingShingle | Lu Liu Kezhen Ying Kebi Wu Si Tang Jin Zhou Zhonghua Cai Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color Journal of Marine Science and Engineering <i>Haematococcus lacustris</i> light color light/dark cycle light color high light intensity |
| title | Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color |
| title_full | Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color |
| title_fullStr | Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color |
| title_full_unstemmed | Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color |
| title_short | Promoting the Growth of <i>Haematococcus lacustris</i> under High Light Intensity through the Combination of Light/Dark Cycle and Light Color |
| title_sort | promoting the growth of i haematococcus lacustris i under high light intensity through the combination of light dark cycle and light color |
| topic | <i>Haematococcus lacustris</i> light color light/dark cycle light color high light intensity |
| url | https://www.mdpi.com/2077-1312/10/7/839 |
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