Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.

HIV-1 infection is associated with a progressive loss of T cell functional capacity and reduced responsiveness to antigenic stimuli. The mechanisms underlying T cell dysfunction in HIV-1/AIDS are not completely understood. Multiple studies have shown that binding of program death ligand 1 (PD-L1) on...

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Main Authors: Nathan L Bowers, E Scott Helton, Richard P H Huijbregts, Paul A Goepfert, Sonya L Heath, Zdenek Hel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-03-01
Series:PLoS Pathogens
Online Access:http://europepmc.org/articles/PMC3953441?pdf=render
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author Nathan L Bowers
E Scott Helton
Richard P H Huijbregts
Paul A Goepfert
Sonya L Heath
Zdenek Hel
author_facet Nathan L Bowers
E Scott Helton
Richard P H Huijbregts
Paul A Goepfert
Sonya L Heath
Zdenek Hel
author_sort Nathan L Bowers
collection DOAJ
description HIV-1 infection is associated with a progressive loss of T cell functional capacity and reduced responsiveness to antigenic stimuli. The mechanisms underlying T cell dysfunction in HIV-1/AIDS are not completely understood. Multiple studies have shown that binding of program death ligand 1 (PD-L1) on the surface of monocytes and dendritic cells to PD-1 on T cells negatively regulates T cell function. Here we show that neutrophils in the blood of HIV-1-infected individuals express high levels of PD-L1. PD-L1 is induced by HIV-1 virions, TLR-7/8 ligand, bacterial lipopolysaccharide (LPS), and IFNα. Neutrophil PD-L1 levels correlate with the expression of PD-1 and CD57 on CD4+ and CD8+ T cells, elevated levels of neutrophil degranulation markers in plasma, and increased frequency of low density neutrophils (LDNs) expressing the phenotype of granulocytic myeloid-derived suppressor cells (G-MDSCs). Neutrophils purified from the blood of HIV-1-infected patients suppress T cell function via several mechanisms including PD-L1/PD-1 interaction and production of reactive oxygen species (ROS). Collectively, the accumulated data suggest that chronic HIV-1 infection results in an induction of immunosuppressive activity of neutrophils characterized by high expression of PD-L1 and an inhibitory effect on T cell function.
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spelling doaj.art-83303f7c6dcb42d383f87d587b4720f72022-12-22T00:13:14ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-03-01103e100399310.1371/journal.ppat.1003993Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.Nathan L BowersE Scott HeltonRichard P H HuijbregtsPaul A GoepfertSonya L HeathZdenek HelHIV-1 infection is associated with a progressive loss of T cell functional capacity and reduced responsiveness to antigenic stimuli. The mechanisms underlying T cell dysfunction in HIV-1/AIDS are not completely understood. Multiple studies have shown that binding of program death ligand 1 (PD-L1) on the surface of monocytes and dendritic cells to PD-1 on T cells negatively regulates T cell function. Here we show that neutrophils in the blood of HIV-1-infected individuals express high levels of PD-L1. PD-L1 is induced by HIV-1 virions, TLR-7/8 ligand, bacterial lipopolysaccharide (LPS), and IFNα. Neutrophil PD-L1 levels correlate with the expression of PD-1 and CD57 on CD4+ and CD8+ T cells, elevated levels of neutrophil degranulation markers in plasma, and increased frequency of low density neutrophils (LDNs) expressing the phenotype of granulocytic myeloid-derived suppressor cells (G-MDSCs). Neutrophils purified from the blood of HIV-1-infected patients suppress T cell function via several mechanisms including PD-L1/PD-1 interaction and production of reactive oxygen species (ROS). Collectively, the accumulated data suggest that chronic HIV-1 infection results in an induction of immunosuppressive activity of neutrophils characterized by high expression of PD-L1 and an inhibitory effect on T cell function.http://europepmc.org/articles/PMC3953441?pdf=render
spellingShingle Nathan L Bowers
E Scott Helton
Richard P H Huijbregts
Paul A Goepfert
Sonya L Heath
Zdenek Hel
Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
PLoS Pathogens
title Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
title_full Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
title_fullStr Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
title_full_unstemmed Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
title_short Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
title_sort immune suppression by neutrophils in hiv 1 infection role of pd l1 pd 1 pathway
url http://europepmc.org/articles/PMC3953441?pdf=render
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