MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells
The craniofacial skeleton is the foundation of most stomatological treatments, including prosthodontics and maxillofacial surgery. Although histologically similar to the appendicular skeleton, the craniofacial skeleton manifests many unique properties in response to external stimuli and signals. How...
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PeerJ Inc.
2019-01-01
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| author | Xiao Hong Yang Kun Yang Yu Lin An Li Bo Wang Guo Luo Xiao Hua Hu |
| author_facet | Xiao Hong Yang Kun Yang Yu Lin An Li Bo Wang Guo Luo Xiao Hua Hu |
| author_sort | Xiao Hong Yang |
| collection | DOAJ |
| description | The craniofacial skeleton is the foundation of most stomatological treatments, including prosthodontics and maxillofacial surgery. Although histologically similar to the appendicular skeleton, the craniofacial skeleton manifests many unique properties in response to external stimuli and signals. However, the mandibular or maxillary bone marrow mesenchyme, which is the intrinsic foundation of the functions of craniofacial skeleton, has not been well studied, and its homeostasis mechanism remains elusive. Osteoporosis is a systemic disease that affects all skeletons and is characterized by bone mass loss. Osteoporotic bone marrow mesenchymal stem cells (BMMSCs) exhibit disturbed homeostasis and distorted lineage commitment. Many reports have shown that microRNAs (miRNAs) play important roles in regulating MSCs homeostasis. Here, to obtain a better understanding of mandibular bone marrow MSCs homeostasis, we isolated and cultured mandible marrow MSCs from mouse mandibles. Using miR-705 mimics and an inhibitor, we demonstrated that miR-705 played a vital role in shifting the mandibular MSCs lineage commitment in vitro. Utilizing an osteoporosis mouse model, we demonstrated that MSCs from ovariectomized (OVX) mouse mandibular bone marrow exhibited impaired osteogenic and excessive adipogenic differentiation. miR-705 was found overexpressed in OVX mandibular MSCs. The knock down of miR-705 in vitro partially attenuated the differentiation disorder of the OVX mandibular MSCs by upregulating the expression of osteogenic marker genes but suppressing adipogenic genes. Taken together, our findings provide a better understanding of the homeostasis mechanism of mandibular BMMSCs and a novel potential therapeutic target for treating mandibular osteoporosis. |
| first_indexed | 2024-03-09T06:44:27Z |
| format | Article |
| id | doaj.art-8331179e9afa49a4990d61eba5202038 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:44:27Z |
| publishDate | 2019-01-01 |
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| spelling | doaj.art-8331179e9afa49a4990d61eba52020382023-12-03T10:41:10ZengPeerJ Inc.PeerJ2167-83592019-01-017e627910.7717/peerj.6279MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cellsXiao Hong Yang0Kun Yang1Yu Lin An2Li Bo Wang3Guo Luo4Xiao Hua Hu5Department of Prosthetics, the Affiliated Stomatology Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Periodontology, the Affiliated Stomatology Hospital of Zunyi Medical University, Zunyi Medical university, Zunyi, Guizhou, ChinaDepartment of Stomatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, ChinaDepartment of Prosthetics, the Affiliated Stomatology Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Stomatology, Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaThe craniofacial skeleton is the foundation of most stomatological treatments, including prosthodontics and maxillofacial surgery. Although histologically similar to the appendicular skeleton, the craniofacial skeleton manifests many unique properties in response to external stimuli and signals. However, the mandibular or maxillary bone marrow mesenchyme, which is the intrinsic foundation of the functions of craniofacial skeleton, has not been well studied, and its homeostasis mechanism remains elusive. Osteoporosis is a systemic disease that affects all skeletons and is characterized by bone mass loss. Osteoporotic bone marrow mesenchymal stem cells (BMMSCs) exhibit disturbed homeostasis and distorted lineage commitment. Many reports have shown that microRNAs (miRNAs) play important roles in regulating MSCs homeostasis. Here, to obtain a better understanding of mandibular bone marrow MSCs homeostasis, we isolated and cultured mandible marrow MSCs from mouse mandibles. Using miR-705 mimics and an inhibitor, we demonstrated that miR-705 played a vital role in shifting the mandibular MSCs lineage commitment in vitro. Utilizing an osteoporosis mouse model, we demonstrated that MSCs from ovariectomized (OVX) mouse mandibular bone marrow exhibited impaired osteogenic and excessive adipogenic differentiation. miR-705 was found overexpressed in OVX mandibular MSCs. The knock down of miR-705 in vitro partially attenuated the differentiation disorder of the OVX mandibular MSCs by upregulating the expression of osteogenic marker genes but suppressing adipogenic genes. Taken together, our findings provide a better understanding of the homeostasis mechanism of mandibular BMMSCs and a novel potential therapeutic target for treating mandibular osteoporosis.https://peerj.com/articles/6279.pdfMicroRNA-705DifferentiationBone marrow mesenchymal stem cellsOsteoporosisMandible boneOsteogenesis |
| spellingShingle | Xiao Hong Yang Kun Yang Yu Lin An Li Bo Wang Guo Luo Xiao Hua Hu MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells PeerJ MicroRNA-705 Differentiation Bone marrow mesenchymal stem cells Osteoporosis Mandible bone Osteogenesis |
| title | MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| title_full | MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| title_fullStr | MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| title_full_unstemmed | MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| title_short | MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| title_sort | microrna 705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells |
| topic | MicroRNA-705 Differentiation Bone marrow mesenchymal stem cells Osteoporosis Mandible bone Osteogenesis |
| url | https://peerj.com/articles/6279.pdf |
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