Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice
<p>Abstract</p> <p>Background</p> <p>Obesity is an important cardiovascular risk factor. This study tested the effect of obesity reduction on preserving left ventricular ejection fraction (LVEF) and attenuating inflammation, oxidative stress and LV remodeling in obese m...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://www.translational-medicine.com/content/10/1/145 |
| _version_ | 1819139391731269632 |
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| author | Wang Hui-Ting Liu Chu-Feng Tsai Tzu-Hsien Chen Yung-Lung Chang Hsueh-Wen Tsai Ching-Yen Leu Steve Zhen Yen-Yi Chai Han-Tan Chung Sheng-Ying Chua Sarah Yen Chia-Hung Yip Hon-Kan |
| author_facet | Wang Hui-Ting Liu Chu-Feng Tsai Tzu-Hsien Chen Yung-Lung Chang Hsueh-Wen Tsai Ching-Yen Leu Steve Zhen Yen-Yi Chai Han-Tan Chung Sheng-Ying Chua Sarah Yen Chia-Hung Yip Hon-Kan |
| author_sort | Wang Hui-Ting |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Obesity is an important cardiovascular risk factor. This study tested the effect of obesity reduction on preserving left ventricular ejection fraction (LVEF) and attenuating inflammation, oxidative stress and LV remodeling in obese mice.</p> <p>Methods and results</p> <p>Eight-week-old C57BL/6 J mice (n=24) were equally divided into control (fed a control diet for 22 weeks), obesity (high-fat diet, 22 weeks), and obese reduction (OR) (high-fat diet, 14 weeks; then control diet, 8 weeks). Animals were sacrificed at post 22-week high-fat diet and the LV myocardium collected. Heart weight, body weight, abdominal-fat weight, total cholesterol level and fasting blood glucose were higher in obesity than in control and OR (all p<0.001). Inflammation measured by mRNA expressions of IL-6, MMP-9, PAI-1 and leptin and protein expression of NF-κB was higher, whereas anti-inflammation measured by mRNA expressions of adiponectin and INF-γ was lower in obesity than in control and OR (all p<0.003). Oxidative protein expressions of NOX-1, NOX-2 and oxidized protein were higher, whereas expression of anti-oxidant markers HO-1 and NQO-1 were lower (all p<0.01); and apoptosis measured by Bax and caspase 3 was higher, whereas anti-apoptotic Bcl-2 was lower in obesity as compared with control and OR (all p<0.001). The expressions of fibrotic markers phosphorylated Smad3 and TGF-β were higher, whereas expression of anti-fibrotic phosphorylated Smad1/5 and BMP-2 were lower (all p<0.02); and LVEF was lower, whereas the LV remodeling was higher in obesity than in control and OR (all p<0.001).</p> <p>Conclusion</p> <p>Impaired LVEF, enhanced LV remodeling, inflammation, fibrosis, oxidative stress and apoptosis were reversed by reduction in mouse obesity.</p> |
| first_indexed | 2024-12-22T11:21:55Z |
| format | Article |
| id | doaj.art-83311a05914c4ac681b0ff8a3181dc1f |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-22T11:21:55Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
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| series | Journal of Translational Medicine |
| spelling | doaj.art-83311a05914c4ac681b0ff8a3181dc1f2022-12-21T18:27:51ZengBMCJournal of Translational Medicine1479-58762012-07-0110114510.1186/1479-5876-10-145Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese miceWang Hui-TingLiu Chu-FengTsai Tzu-HsienChen Yung-LungChang Hsueh-WenTsai Ching-YenLeu SteveZhen Yen-YiChai Han-TanChung Sheng-YingChua SarahYen Chia-HungYip Hon-Kan<p>Abstract</p> <p>Background</p> <p>Obesity is an important cardiovascular risk factor. This study tested the effect of obesity reduction on preserving left ventricular ejection fraction (LVEF) and attenuating inflammation, oxidative stress and LV remodeling in obese mice.</p> <p>Methods and results</p> <p>Eight-week-old C57BL/6 J mice (n=24) were equally divided into control (fed a control diet for 22 weeks), obesity (high-fat diet, 22 weeks), and obese reduction (OR) (high-fat diet, 14 weeks; then control diet, 8 weeks). Animals were sacrificed at post 22-week high-fat diet and the LV myocardium collected. Heart weight, body weight, abdominal-fat weight, total cholesterol level and fasting blood glucose were higher in obesity than in control and OR (all p<0.001). Inflammation measured by mRNA expressions of IL-6, MMP-9, PAI-1 and leptin and protein expression of NF-κB was higher, whereas anti-inflammation measured by mRNA expressions of adiponectin and INF-γ was lower in obesity than in control and OR (all p<0.003). Oxidative protein expressions of NOX-1, NOX-2 and oxidized protein were higher, whereas expression of anti-oxidant markers HO-1 and NQO-1 were lower (all p<0.01); and apoptosis measured by Bax and caspase 3 was higher, whereas anti-apoptotic Bcl-2 was lower in obesity as compared with control and OR (all p<0.001). The expressions of fibrotic markers phosphorylated Smad3 and TGF-β were higher, whereas expression of anti-fibrotic phosphorylated Smad1/5 and BMP-2 were lower (all p<0.02); and LVEF was lower, whereas the LV remodeling was higher in obesity than in control and OR (all p<0.001).</p> <p>Conclusion</p> <p>Impaired LVEF, enhanced LV remodeling, inflammation, fibrosis, oxidative stress and apoptosis were reversed by reduction in mouse obesity.</p>http://www.translational-medicine.com/content/10/1/145ObesityInflammationOxidative stressApoptosisFibrosis |
| spellingShingle | Wang Hui-Ting Liu Chu-Feng Tsai Tzu-Hsien Chen Yung-Lung Chang Hsueh-Wen Tsai Ching-Yen Leu Steve Zhen Yen-Yi Chai Han-Tan Chung Sheng-Ying Chua Sarah Yen Chia-Hung Yip Hon-Kan Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice Journal of Translational Medicine Obesity Inflammation Oxidative stress Apoptosis Fibrosis |
| title | Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice |
| title_full | Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice |
| title_fullStr | Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice |
| title_full_unstemmed | Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice |
| title_short | Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice |
| title_sort | effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling oxidative stress and inflammation in obese mice |
| topic | Obesity Inflammation Oxidative stress Apoptosis Fibrosis |
| url | http://www.translational-medicine.com/content/10/1/145 |
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