6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model
Objective: To explore the effects of 6-gingerol, asarinin, and deoxyschizandrin—the main components of Zingiber officinale (Willd.) Rosc. (Gan Jiang), Asarum heterotropoides f. var. mandshuricum (Maxim.) (Xi Xin), and Schisandra chinensis (Turcz.) Baill. (Wu Wei Zi), respectively—on an interleukin (...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754823000030 |
| _version_ | 1797701811424985088 |
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| author | Shuai Li Shuzhen Guo Dayu Lin Jiang Yu Jiayang Tang Xiaoxi lv Yuping Zhang Qingqing Wang |
| author_facet | Shuai Li Shuzhen Guo Dayu Lin Jiang Yu Jiayang Tang Xiaoxi lv Yuping Zhang Qingqing Wang |
| author_sort | Shuai Li |
| collection | DOAJ |
| description | Objective: To explore the effects of 6-gingerol, asarinin, and deoxyschizandrin—the main components of Zingiber officinale (Willd.) Rosc. (Gan Jiang), Asarum heterotropoides f. var. mandshuricum (Maxim.) (Xi Xin), and Schisandra chinensis (Turcz.) Baill. (Wu Wei Zi), respectively—on an interleukin (IL)-13–induced BEAS-2B cell model in vitro. Methods: The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1% fetal bovine serum (FBS) in vitro. Mitoquinone mesylate (Mito-Q) treatment was used as a positive control group, and different concentrations of 6-gingerol, asarinin, and deoxyschizandrin were used to treat the models. The level of reactive oxygen species (ROS) production was detected by flow cytometry. The expression levels of LC3B, Beclin1, adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK), phosphorylated-AMP–activated protein kinase (P-AMPK), dynamin-related protein 1 (DRP1), and mitochondrial fusion protein 2 (MFN2) were detected by Western blot. Mitochondrial membrane potential (MMP) assay kit with JC-1 was utilized to detect the level of MMP. Results: The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1% FBS showed an increased ROS level and a decreased MMP. 6-Gingerol, asarinin, and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model. Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B, while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model. Conclusion: 6-Gingerol, asarinin, and deoxyschizandrin can reduce ROS generation and increase MMP, but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein. The three components have both synergistic and complementary effects in classic medicine compatibility. This study may provide an innovative strategy to reduce the lung inflammation related to IL-13. |
| first_indexed | 2024-03-12T04:41:07Z |
| format | Article |
| id | doaj.art-833881b287ab4a588839fe2cfa834df9 |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T04:41:07Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-833881b287ab4a588839fe2cfa834df92023-09-03T09:41:12ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482023-04-011022182276-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell modelShuai Li0Shuzhen Guo1Dayu Lin2Jiang Yu3Jiayang Tang4Xiaoxi lv5Yuping Zhang6Qingqing Wang7School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaInstitute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, China; Corresponding author.Objective: To explore the effects of 6-gingerol, asarinin, and deoxyschizandrin—the main components of Zingiber officinale (Willd.) Rosc. (Gan Jiang), Asarum heterotropoides f. var. mandshuricum (Maxim.) (Xi Xin), and Schisandra chinensis (Turcz.) Baill. (Wu Wei Zi), respectively—on an interleukin (IL)-13–induced BEAS-2B cell model in vitro. Methods: The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1% fetal bovine serum (FBS) in vitro. Mitoquinone mesylate (Mito-Q) treatment was used as a positive control group, and different concentrations of 6-gingerol, asarinin, and deoxyschizandrin were used to treat the models. The level of reactive oxygen species (ROS) production was detected by flow cytometry. The expression levels of LC3B, Beclin1, adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK), phosphorylated-AMP–activated protein kinase (P-AMPK), dynamin-related protein 1 (DRP1), and mitochondrial fusion protein 2 (MFN2) were detected by Western blot. Mitochondrial membrane potential (MMP) assay kit with JC-1 was utilized to detect the level of MMP. Results: The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1% FBS showed an increased ROS level and a decreased MMP. 6-Gingerol, asarinin, and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model. Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B, while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model. Conclusion: 6-Gingerol, asarinin, and deoxyschizandrin can reduce ROS generation and increase MMP, but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein. The three components have both synergistic and complementary effects in classic medicine compatibility. This study may provide an innovative strategy to reduce the lung inflammation related to IL-13.http://www.sciencedirect.com/science/article/pii/S2095754823000030Interleukin-13Mitochondrial functionMitoquinone mesylate6-GingerolAsarininDeoxyschizandrin |
| spellingShingle | Shuai Li Shuzhen Guo Dayu Lin Jiang Yu Jiayang Tang Xiaoxi lv Yuping Zhang Qingqing Wang 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model Journal of Traditional Chinese Medical Sciences Interleukin-13 Mitochondrial function Mitoquinone mesylate 6-Gingerol Asarinin Deoxyschizandrin |
| title | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model |
| title_full | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model |
| title_fullStr | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model |
| title_full_unstemmed | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model |
| title_short | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13–induced BEAS-2B cell model |
| title_sort | 6 gingerol asarinin and deoxyschizandrin improve bronchial epithelium functions in an interleukin 13 induced beas 2b cell model |
| topic | Interleukin-13 Mitochondrial function Mitoquinone mesylate 6-Gingerol Asarinin Deoxyschizandrin |
| url | http://www.sciencedirect.com/science/article/pii/S2095754823000030 |
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