Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility
Background: The purpose of the study was to describe the epidemiological implication of <i>Pseudomonas aeruginosa</i> between 2017–2022 in a tertiary hospital from Romania, including the molecular fingerprinting of similar phenotypic strains (multidrug-resistant isolates), which would ha...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/12/2049 |
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| author | Răzvan Lucian Coșeriu Camelia Vintilă Anca Delia Mare Cristina Nicoleta Ciurea Radu Ovidiu Togănel Anca Cighir Anastasia Simion Adrian Man |
| author_facet | Răzvan Lucian Coșeriu Camelia Vintilă Anca Delia Mare Cristina Nicoleta Ciurea Radu Ovidiu Togănel Anca Cighir Anastasia Simion Adrian Man |
| author_sort | Răzvan Lucian Coșeriu |
| collection | DOAJ |
| description | Background: The purpose of the study was to describe the epidemiological implication of <i>Pseudomonas aeruginosa</i> between 2017–2022 in a tertiary hospital from Romania, including the molecular fingerprinting of similar phenotypic strains (multidrug-resistant isolates), which would have an important health impact. The study also describes the resistance profile of <i>P. aeruginosa</i> before and during COVID-19, which might bring new information regarding the management of antibiotic treatments. Materials and methods: Information regarding wards, specimen types, species, and antibiotic resistance profile of 1994 strains of <i>Pseudomonas</i> spp. Isolated over a period of 6 years in Mures Clinical County Hospital, Romania, was collected from the WHONET database. From 50 multidrug-resistant isolates, molecular fingerprinting was performed by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) to prove the potential clonal distribution. Results: A number of 1994 <i>Pseudomonas</i> spp. were isolated between 2017–2022, from which <i>P. aeruginosa</i> was the most frequent species, 97.39% (n = 1942). <i>P. aeruginosa</i> was most frequently isolated in 2017 (n = 538), with the dermatology department as the main source, mainly from pus secretion. A drop in the harvesting rate was noted in 2020 due to COVID-19 restrictions. Regarding the resistance profile, there are a few modifications. The susceptibility of <i>P. aeruginosa</i> to carbapenems, piperacillin-tazobactam, and amikacin suffered alterations before and during COVID-19. The molecular fingerprinting showed three <i>P. aeruginosa</i> clusters, including strains with 80–99% similarity. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:11:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-833b4944fcbd462cb6c6ec17347289502023-11-24T16:12:41ZengMDPI AGLife2075-17292022-12-011212204910.3390/life12122049Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to SusceptibilityRăzvan Lucian Coșeriu0Camelia Vintilă1Anca Delia Mare2Cristina Nicoleta Ciurea3Radu Ovidiu Togănel4Anca Cighir5Anastasia Simion6Adrian Man7Department of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaDepartment of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology Târgu Mureș, 540139 Targu Mures, RomaniaBackground: The purpose of the study was to describe the epidemiological implication of <i>Pseudomonas aeruginosa</i> between 2017–2022 in a tertiary hospital from Romania, including the molecular fingerprinting of similar phenotypic strains (multidrug-resistant isolates), which would have an important health impact. The study also describes the resistance profile of <i>P. aeruginosa</i> before and during COVID-19, which might bring new information regarding the management of antibiotic treatments. Materials and methods: Information regarding wards, specimen types, species, and antibiotic resistance profile of 1994 strains of <i>Pseudomonas</i> spp. Isolated over a period of 6 years in Mures Clinical County Hospital, Romania, was collected from the WHONET database. From 50 multidrug-resistant isolates, molecular fingerprinting was performed by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) to prove the potential clonal distribution. Results: A number of 1994 <i>Pseudomonas</i> spp. were isolated between 2017–2022, from which <i>P. aeruginosa</i> was the most frequent species, 97.39% (n = 1942). <i>P. aeruginosa</i> was most frequently isolated in 2017 (n = 538), with the dermatology department as the main source, mainly from pus secretion. A drop in the harvesting rate was noted in 2020 due to COVID-19 restrictions. Regarding the resistance profile, there are a few modifications. The susceptibility of <i>P. aeruginosa</i> to carbapenems, piperacillin-tazobactam, and amikacin suffered alterations before and during COVID-19. The molecular fingerprinting showed three <i>P. aeruginosa</i> clusters, including strains with 80–99% similarity.https://www.mdpi.com/2075-1729/12/12/2049<i>Pseudomonas aeruginosa</i>ERIC-PCRbacterial resistance profilesCOVID-19 |
| spellingShingle | Răzvan Lucian Coșeriu Camelia Vintilă Anca Delia Mare Cristina Nicoleta Ciurea Radu Ovidiu Togănel Anca Cighir Anastasia Simion Adrian Man Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility Life <i>Pseudomonas aeruginosa</i> ERIC-PCR bacterial resistance profiles COVID-19 |
| title | Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility |
| title_full | Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility |
| title_fullStr | Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility |
| title_full_unstemmed | Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility |
| title_short | Epidemiology, Evolution of Antimicrobial Profile and Genomic Fingerprints of <i>Pseudomonas aeruginosa</i> before and during COVID-19: Transition from Resistance to Susceptibility |
| title_sort | epidemiology evolution of antimicrobial profile and genomic fingerprints of i pseudomonas aeruginosa i before and during covid 19 transition from resistance to susceptibility |
| topic | <i>Pseudomonas aeruginosa</i> ERIC-PCR bacterial resistance profiles COVID-19 |
| url | https://www.mdpi.com/2075-1729/12/12/2049 |
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