Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model
Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffol...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-03-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00083/full |
| _version_ | 1818477104514203648 |
|---|---|
| author | Joana Castro Aliona S. Rosca Piet Cools Mario Vaneechoutte Nuno Cerca |
| author_facet | Joana Castro Aliona S. Rosca Piet Cools Mario Vaneechoutte Nuno Cerca |
| author_sort | Joana Castro |
| collection | DOAJ |
| description | Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV. |
| first_indexed | 2024-12-10T09:33:53Z |
| format | Article |
| id | doaj.art-833d4ec550e24556a30474d78c3a0ac2 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T09:33:53Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-833d4ec550e24556a30474d78c3a0ac22022-12-22T01:54:14ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-03-011010.3389/fcimb.2020.00083520800Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro ModelJoana Castro0Aliona S. Rosca1Piet Cools2Mario Vaneechoutte3Nuno Cerca4Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), Centre of Biological Engineering (CEB), Braga, PortugalLaboratory of Research in Biofilms Rosário Oliveira (LIBRO), Centre of Biological Engineering (CEB), Braga, PortugalLaboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumLaboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumLaboratory of Research in Biofilms Rosário Oliveira (LIBRO), Centre of Biological Engineering (CEB), Braga, PortugalBacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV.https://www.frontiersin.org/article/10.3389/fcimb.2020.00083/fullbacterial vaginosispolymicrobial biofilmsGardnerellaAtopobium vaginaePNA-FISH |
| spellingShingle | Joana Castro Aliona S. Rosca Piet Cools Mario Vaneechoutte Nuno Cerca Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model Frontiers in Cellular and Infection Microbiology bacterial vaginosis polymicrobial biofilms Gardnerella Atopobium vaginae PNA-FISH |
| title | Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model |
| title_full | Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model |
| title_fullStr | Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model |
| title_full_unstemmed | Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model |
| title_short | Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model |
| title_sort | gardnerella vaginalis enhances atopobium vaginae viability in an in vitro model |
| topic | bacterial vaginosis polymicrobial biofilms Gardnerella Atopobium vaginae PNA-FISH |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00083/full |
| work_keys_str_mv | AT joanacastro gardnerellavaginalisenhancesatopobiumvaginaeviabilityinaninvitromodel AT alionasrosca gardnerellavaginalisenhancesatopobiumvaginaeviabilityinaninvitromodel AT pietcools gardnerellavaginalisenhancesatopobiumvaginaeviabilityinaninvitromodel AT mariovaneechoutte gardnerellavaginalisenhancesatopobiumvaginaeviabilityinaninvitromodel AT nunocerca gardnerellavaginalisenhancesatopobiumvaginaeviabilityinaninvitromodel |