Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
Summary: Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724001096 |
| _version_ | 1797263616649461760 |
|---|---|
| author | Suruchi Roychoudhry Jean T. Greenberg Nicolás M. Cecchini |
| author_facet | Suruchi Roychoudhry Jean T. Greenberg Nicolás M. Cecchini |
| author_sort | Suruchi Roychoudhry |
| collection | DOAJ |
| description | Summary: Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.For complete details on the use and execution of this protocol, please refer to Cecchini et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-25T00:15:50Z |
| format | Article |
| id | doaj.art-833dc40b96c94e168520df90eed19fae |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-25T00:15:50Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-833dc40b96c94e168520df90eed19fae2024-03-13T04:46:25ZengElsevierSTAR Protocols2666-16672024-06-0152102944Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in ArabidopsisSuruchi Roychoudhry0Jean T. Greenberg1Nicolás M. Cecchini2Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UKDepartment of Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS 524W, Chicago, IL 60637, USA; Corresponding authorCentro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, CONICET, Departamento de Química Biológica-Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, Córdoba X5000HUA, Argentina; Corresponding authorSummary: Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.For complete details on the use and execution of this protocol, please refer to Cecchini et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724001096Model OrganismsPlant sciencesSignal Transduction |
| spellingShingle | Suruchi Roychoudhry Jean T. Greenberg Nicolás M. Cecchini Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis STAR Protocols Model Organisms Plant sciences Signal Transduction |
| title | Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis |
| title_full | Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis |
| title_fullStr | Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis |
| title_full_unstemmed | Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis |
| title_short | Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis |
| title_sort | protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in arabidopsis |
| topic | Model Organisms Plant sciences Signal Transduction |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724001096 |
| work_keys_str_mv | AT suruchiroychoudhry protocolforanalyzingthemovementanduptakeofisotopicallylabeledsignalingmoleculeazelaicacidinarabidopsis AT jeantgreenberg protocolforanalyzingthemovementanduptakeofisotopicallylabeledsignalingmoleculeazelaicacidinarabidopsis AT nicolasmcecchini protocolforanalyzingthemovementanduptakeofisotopicallylabeledsignalingmoleculeazelaicacidinarabidopsis |