Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid Leukemia Compared with Normal Individuals
Background: Chronic myeloid leukemia is a type of myeloproliferative malignancy that is associated with translocation between chromosomes 9 and 22 and accounts for approximately 15% of all leukemia. SNHGs are a subset of long non-coding RNAs that are considered to host small nuclear RNAs. SNHG5 is a...
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Isfahan University of Medical Sciences
2022-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | https://jims.mui.ac.ir/article_16290_88f7a963505a869b306ce7f2a29192a2.pdf |
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| author | Zahra Shahpouri-Arani Mohamad Reza Hajari Javad Mohammadi-Asl Najmaldin Saki |
| author_facet | Zahra Shahpouri-Arani Mohamad Reza Hajari Javad Mohammadi-Asl Najmaldin Saki |
| author_sort | Zahra Shahpouri-Arani |
| collection | DOAJ |
| description | Background: Chronic myeloid leukemia is a type of myeloproliferative malignancy that is associated with translocation between chromosomes 9 and 22 and accounts for approximately 15% of all leukemia. SNHGs are a subset of long non-coding RNAs that are considered to host small nuclear RNAs. SNHG5 is a regulatory factor in gene expression in various cancers, including chronic myeloid leukemia. The aim of this study was to evaluate and compare the expression level of SNHG5 in the peripheral blood sample of patients with chronic myeloid leukemia compared to normal individuals and then to evaluate SNHG5 as a potential biomarker in these patients. Methods: This study was conducted with a case-control design. First, the accuracy of chronic myeloid leukemia was confirmed using karyotype, molecular and cellular methods. After RNA extraction from white blood cells and cDNA synthesis, gene expression was measured using Real Time PCR. Finally, the data were statistically analyzed.Findings: Findings of this study showed that the expression level of SNHG5 in patients with chronic myeloid leukemia significantly increased compared to normal individuals (P < 0.001). Increased expression may indicate the potential role of this molecule in the development and progression of this cancer.Conclusion: The present study demonstrates the effective role of SNHG5 in the progression of chronic myeloid leukemia and can be investigated as a biomarker and potential therapeutic target in future studies |
| first_indexed | 2024-03-12T05:13:49Z |
| format | Article |
| id | doaj.art-834111a6f3834c2c9f4fad17b076600f |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T05:13:49Z |
| publishDate | 2022-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-834111a6f3834c2c9f4fad17b076600f2023-09-03T08:21:57ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2022-03-0140659626810.48305/jims.2022.1629016290Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid Leukemia Compared with Normal IndividualsZahra Shahpouri-Arani0Mohamad Reza Hajari1Javad Mohammadi-Asl2Najmaldin Saki3MSc, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssistant Professor, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssistant Professor, Department of Genetics, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, IranAssociate Professor, Department of Laboratory Sciences, School of Paramedical Sciences, Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Chronic myeloid leukemia is a type of myeloproliferative malignancy that is associated with translocation between chromosomes 9 and 22 and accounts for approximately 15% of all leukemia. SNHGs are a subset of long non-coding RNAs that are considered to host small nuclear RNAs. SNHG5 is a regulatory factor in gene expression in various cancers, including chronic myeloid leukemia. The aim of this study was to evaluate and compare the expression level of SNHG5 in the peripheral blood sample of patients with chronic myeloid leukemia compared to normal individuals and then to evaluate SNHG5 as a potential biomarker in these patients. Methods: This study was conducted with a case-control design. First, the accuracy of chronic myeloid leukemia was confirmed using karyotype, molecular and cellular methods. After RNA extraction from white blood cells and cDNA synthesis, gene expression was measured using Real Time PCR. Finally, the data were statistically analyzed.Findings: Findings of this study showed that the expression level of SNHG5 in patients with chronic myeloid leukemia significantly increased compared to normal individuals (P < 0.001). Increased expression may indicate the potential role of this molecule in the development and progression of this cancer.Conclusion: The present study demonstrates the effective role of SNHG5 in the progression of chronic myeloid leukemia and can be investigated as a biomarker and potential therapeutic target in future studieshttps://jims.mui.ac.ir/article_16290_88f7a963505a869b306ce7f2a29192a2.pdfbiomarkerschronic myeloid leukemiacase- control studieslong non-coding rna snhg5 |
| spellingShingle | Zahra Shahpouri-Arani Mohamad Reza Hajari Javad Mohammadi-Asl Najmaldin Saki Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid Leukemia Compared with Normal Individuals مجله دانشکده پزشکی اصفهان biomarkers chronic myeloid leukemia case- control studies long non-coding rna snhg5 |
| title | Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid
Leukemia Compared with Normal Individuals |
| title_full | Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid
Leukemia Compared with Normal Individuals |
| title_fullStr | Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid
Leukemia Compared with Normal Individuals |
| title_full_unstemmed | Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid
Leukemia Compared with Normal Individuals |
| title_short | Up-regulation of SNHG5 Expression Level in Patients with Chronic Myeloid
Leukemia Compared with Normal Individuals |
| title_sort | up regulation of snhg5 expression level in patients with chronic myeloid leukemia compared with normal individuals |
| topic | biomarkers chronic myeloid leukemia case- control studies long non-coding rna snhg5 |
| url | https://jims.mui.ac.ir/article_16290_88f7a963505a869b306ce7f2a29192a2.pdf |
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