Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis
Abstract Sepsis is a life-threatening multiple-organ injury caused by disordered host immune response to microbial infection. However, the correlation between gut microbiota dysbiosis and immune indicators remains unexplored. To address this gap in knowledge, we carried out 16 S rDNA sequencing, ana...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Pediatrics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12887-023-04349-8 |
| _version_ | 1797451484546203648 |
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| author | Xia Lin Mohnad Abdalla Junjie Yang Lei Liu Yali Fu Yanli Zhang Shuchun Yang Han Yu Yongsheng Ge Sufang Zhang Guiyun Kang Wei Dang Qin Jiang Ying Wang Zhongtao Gai |
| author_facet | Xia Lin Mohnad Abdalla Junjie Yang Lei Liu Yali Fu Yanli Zhang Shuchun Yang Han Yu Yongsheng Ge Sufang Zhang Guiyun Kang Wei Dang Qin Jiang Ying Wang Zhongtao Gai |
| author_sort | Xia Lin |
| collection | DOAJ |
| description | Abstract Sepsis is a life-threatening multiple-organ injury caused by disordered host immune response to microbial infection. However, the correlation between gut microbiota dysbiosis and immune indicators remains unexplored. To address this gap in knowledge, we carried out 16 S rDNA sequencing, analyzed clinical fecal samples from children with sepsis (n = 30) and control children (n = 25), and obtained immune indicators, including T cell subtypes (CD3+, CD3+CD4+, CD3+CD8+, and CD4/CD8), NK cells, cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ), and immunoglobulin indices (IgA, IgE, IgM and IgG). In addition, we analyzed the correlation between gut microbiota dysbiosis and immune indicators, and evaluated the clinical discriminatory power of discovered bacterial biomarkers. We found that children with sepsis exhibited gut bacterial dysbiosis and low alpha diversity. The Spearman’s rank correlation coefficient suggested that Rhodococcus erythropolis had a significantly positive correlation with IFN-γ and CD3 + T cells. Klebsiella pneumoniae and Streptococcus mitis were significantly correlated with NK cells. Bacteroides uniformis was significantly positively correlated with IgM and erythrocyte sedimentation rate, and Eubacterium eligens was significantly positively correlated with IL-4 and CD3+CD8+ T cells. The biomarkers discovered in this study had strong discriminatory power. These changes in the gut microbiome may be closely related to immunologic dysfunction and to the development or exacerbation of sepsis. However, a large sample size is required for verification. |
| first_indexed | 2024-03-09T14:54:18Z |
| format | Article |
| id | doaj.art-8346b05e5dab4c7a8f3993b4ea836f5e |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-03-09T14:54:18Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-8346b05e5dab4c7a8f3993b4ea836f5e2023-11-26T14:16:02ZengBMCBMC Pediatrics1471-24312023-10-0123111010.1186/s12887-023-04349-8Relationship between gut microbiota dysbiosis and immune indicator in children with sepsisXia Lin0Mohnad Abdalla1Junjie Yang2Lei Liu3Yali Fu4Yanli Zhang5Shuchun Yang6Han Yu7Yongsheng Ge8Sufang Zhang9Guiyun Kang10Wei Dang11Qin Jiang12Ying Wang13Zhongtao Gai14Children’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityCollege of Life Science, Qilu Normal UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityChildren’s Hospital Affiliated to Shandong UniversityAbstract Sepsis is a life-threatening multiple-organ injury caused by disordered host immune response to microbial infection. However, the correlation between gut microbiota dysbiosis and immune indicators remains unexplored. To address this gap in knowledge, we carried out 16 S rDNA sequencing, analyzed clinical fecal samples from children with sepsis (n = 30) and control children (n = 25), and obtained immune indicators, including T cell subtypes (CD3+, CD3+CD4+, CD3+CD8+, and CD4/CD8), NK cells, cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ), and immunoglobulin indices (IgA, IgE, IgM and IgG). In addition, we analyzed the correlation between gut microbiota dysbiosis and immune indicators, and evaluated the clinical discriminatory power of discovered bacterial biomarkers. We found that children with sepsis exhibited gut bacterial dysbiosis and low alpha diversity. The Spearman’s rank correlation coefficient suggested that Rhodococcus erythropolis had a significantly positive correlation with IFN-γ and CD3 + T cells. Klebsiella pneumoniae and Streptococcus mitis were significantly correlated with NK cells. Bacteroides uniformis was significantly positively correlated with IgM and erythrocyte sedimentation rate, and Eubacterium eligens was significantly positively correlated with IL-4 and CD3+CD8+ T cells. The biomarkers discovered in this study had strong discriminatory power. These changes in the gut microbiome may be closely related to immunologic dysfunction and to the development or exacerbation of sepsis. However, a large sample size is required for verification.https://doi.org/10.1186/s12887-023-04349-8sepsisGut microbiotaImmune indicatorBiomarkerChildren |
| spellingShingle | Xia Lin Mohnad Abdalla Junjie Yang Lei Liu Yali Fu Yanli Zhang Shuchun Yang Han Yu Yongsheng Ge Sufang Zhang Guiyun Kang Wei Dang Qin Jiang Ying Wang Zhongtao Gai Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis BMC Pediatrics sepsis Gut microbiota Immune indicator Biomarker Children |
| title | Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| title_full | Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| title_fullStr | Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| title_full_unstemmed | Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| title_short | Relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| title_sort | relationship between gut microbiota dysbiosis and immune indicator in children with sepsis |
| topic | sepsis Gut microbiota Immune indicator Biomarker Children |
| url | https://doi.org/10.1186/s12887-023-04349-8 |
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