Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations
<p>Abstract</p> <p>Background</p> <p>The association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the pres...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-10-01
|
| Series: | Lipids in Health and Disease |
| Online Access: | http://www.lipidworld.com/content/9/1/123 |
| _version_ | 1818963401248866304 |
|---|---|
| author | Cao Xiao-Li Hu Xi-Jiang Aung Lynn Wu Dong-Feng Miao Lin Liu Wan-Ying Yin Rui-Xing Zhang Lin Wu Jin-Zhen Pan Shang-Ling |
| author_facet | Cao Xiao-Li Hu Xi-Jiang Aung Lynn Wu Dong-Feng Miao Lin Liu Wan-Ying Yin Rui-Xing Zhang Lin Wu Jin-Zhen Pan Shang-Ling |
| author_sort | Cao Xiao-Li |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677T polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.</p> <p>Methods</p> <p>A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples. Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.</p> <p>Results</p> <p>The levels of serum total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han (<it>P </it>< 0.05-0.001). The frequency of C and T alleles was 77.4% and 22.6% in Bai Ku Yao, and 60.9% and 39.1% in Han (<it>P </it>< 0.001); respectively. The frequency of CC, CT and TT genotypes was 58.7%, 37.3% and 4.0% in Bai Ku Yao, and 32.6%, 56.4% and 11.0% in Han (<it>P </it>< 0.001); respectively. The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes (<it>P </it>< 0.05-0.01). The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes (<it>P </it>< 0.05). The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC, TG and LDL-C in Bai Ku Yao were correlated with genotypes (<it>P </it>< 0.05-0.001), whereas the levels of LDL-C in Han were associated with genotypes (<it>P </it>< 0.001). Serum lipid parameters were also correlated with sex, age, body mass index, alcohol consumption, cigarette smoking, and blood pressure in the both ethnic groups.</p> <p>Conclusions</p> <p>The differences in serum TC, TG, LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677T or different MTHFR gene-enviromental interactions.</p> |
| first_indexed | 2024-12-20T12:44:38Z |
| format | Article |
| id | doaj.art-8346b4e813da442999fa7dc0bb0e2d8d |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-20T12:44:38Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-8346b4e813da442999fa7dc0bb0e2d8d2022-12-21T19:40:20ZengBMCLipids in Health and Disease1476-511X2010-10-019112310.1186/1476-511X-9-123Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populationsCao Xiao-LiHu Xi-JiangAung LynnWu Dong-FengMiao LinLiu Wan-YingYin Rui-XingZhang LinWu Jin-ZhenPan Shang-Ling<p>Abstract</p> <p>Background</p> <p>The association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677T polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.</p> <p>Methods</p> <p>A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples. Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.</p> <p>Results</p> <p>The levels of serum total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han (<it>P </it>< 0.05-0.001). The frequency of C and T alleles was 77.4% and 22.6% in Bai Ku Yao, and 60.9% and 39.1% in Han (<it>P </it>< 0.001); respectively. The frequency of CC, CT and TT genotypes was 58.7%, 37.3% and 4.0% in Bai Ku Yao, and 32.6%, 56.4% and 11.0% in Han (<it>P </it>< 0.001); respectively. The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes (<it>P </it>< 0.05-0.01). The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes (<it>P </it>< 0.05). The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC, TG and LDL-C in Bai Ku Yao were correlated with genotypes (<it>P </it>< 0.05-0.001), whereas the levels of LDL-C in Han were associated with genotypes (<it>P </it>< 0.001). Serum lipid parameters were also correlated with sex, age, body mass index, alcohol consumption, cigarette smoking, and blood pressure in the both ethnic groups.</p> <p>Conclusions</p> <p>The differences in serum TC, TG, LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677T or different MTHFR gene-enviromental interactions.</p>http://www.lipidworld.com/content/9/1/123 |
| spellingShingle | Cao Xiao-Li Hu Xi-Jiang Aung Lynn Wu Dong-Feng Miao Lin Liu Wan-Ying Yin Rui-Xing Zhang Lin Wu Jin-Zhen Pan Shang-Ling Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations Lipids in Health and Disease |
| title | Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations |
| title_full | Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations |
| title_fullStr | Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations |
| title_full_unstemmed | Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations |
| title_short | Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations |
| title_sort | association of methylenetetrahydrofolate reductase c677t polymorphism and serum lipid levels in the guangxi bai ku yao and han populations |
| url | http://www.lipidworld.com/content/9/1/123 |
| work_keys_str_mv | AT caoxiaoli associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT huxijiang associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT aunglynn associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT wudongfeng associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT miaolin associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT liuwanying associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT yinruixing associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT zhanglin associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT wujinzhen associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations AT panshangling associationofmethylenetetrahydrofolatereductasec677tpolymorphismandserumlipidlevelsintheguangxibaikuyaoandhanpopulations |