Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with r...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/2304-6732/8/11/495 |
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| author | Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky |
| author_facet | Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky |
| author_sort | Nadezhda Ignatova |
| collection | DOAJ |
| description | Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with renal calculi were isolated and identified using standard methods of bacteriological analysis and tested for drug resistance to 10 antibiotics by the disco-diffusion method. Uropathogenic bacterial strains present in 78.7 ± 5.2% of the infected samples from the total number of analyzed calculi. The most frequent representatives belonged to the genera <i>Staphylococcus</i>, <i>Escherichia</i>, and <i>Enterococcus</i>. All tested strains showed high antibiotic resistance. Representatives of the most common bacterial genera in the calculi were used as models for the selection of PD exposure modes. It was found that the maximum time of photosensitizer accumulation depends on the structure of the bacterial cell wall: 30 min for Gram-negative strains and 60 min for Gram-positive ones. Optimal modes of PD exposure to antibiotic-resistant uropathogenic microorganisms were selected: 50 µg/mL Fotoditazin and 150 mW laser power. The maximal bactericidal activity of PDI against uropathogenic microorganisms was shown for <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>. The bacteriostatic effect was found against <i>Escherichia coli</i> and <i>Proteus mirabilis.</i> |
| first_indexed | 2024-03-10T05:09:05Z |
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| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-10T05:09:05Z |
| publishDate | 2021-11-01 |
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| record_format | Article |
| series | Photonics |
| spelling | doaj.art-83474888f87e42b9a5a7b3af4b6a767e2023-11-23T01:01:31ZengMDPI AGPhotonics2304-67322021-11-0181149510.3390/photonics8110495Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant UropathogensNadezhda Ignatova0Tatiana Ivanova1Artem Antonyan2Ivan Budruev3Olga Streltsova4Vadim Elagin5Vladislav Kamensky6Department of Epidemiology, Microbiology and Evidence-Based Medicine, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaMedical and Preventative Care Department, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaDepartment of Urology Named after E.V. Shakhov, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaInstitute of Biology and Biomedicine, National Research Lobachevsky State University of Nizhny Novgorod, 23 Prospekt Gagarina (Gagarin Avenue), 603022 Nizhny Novgorod, RussiaDepartment of Urology Named after E.V. Shakhov, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603005 Nizhny Novgorod, RussiaPhotodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with renal calculi were isolated and identified using standard methods of bacteriological analysis and tested for drug resistance to 10 antibiotics by the disco-diffusion method. Uropathogenic bacterial strains present in 78.7 ± 5.2% of the infected samples from the total number of analyzed calculi. The most frequent representatives belonged to the genera <i>Staphylococcus</i>, <i>Escherichia</i>, and <i>Enterococcus</i>. All tested strains showed high antibiotic resistance. Representatives of the most common bacterial genera in the calculi were used as models for the selection of PD exposure modes. It was found that the maximum time of photosensitizer accumulation depends on the structure of the bacterial cell wall: 30 min for Gram-negative strains and 60 min for Gram-positive ones. Optimal modes of PD exposure to antibiotic-resistant uropathogenic microorganisms were selected: 50 µg/mL Fotoditazin and 150 mW laser power. The maximal bactericidal activity of PDI against uropathogenic microorganisms was shown for <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>. The bacteriostatic effect was found against <i>Escherichia coli</i> and <i>Proteus mirabilis.</i>https://www.mdpi.com/2304-6732/8/11/495urolithiasisantibiotic-resistant bacteriabactericidal effectlaser-induced inactivationphotosensitizer |
| spellingShingle | Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens Photonics urolithiasis antibiotic-resistant bacteria bactericidal effect laser-induced inactivation photosensitizer |
| title | Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens |
| title_full | Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens |
| title_fullStr | Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens |
| title_full_unstemmed | Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens |
| title_short | Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens |
| title_sort | efficacy of photodynamic inactivation against the major human antibiotic resistant uropathogens |
| topic | urolithiasis antibiotic-resistant bacteria bactericidal effect laser-induced inactivation photosensitizer |
| url | https://www.mdpi.com/2304-6732/8/11/495 |
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