| Summary: | Objective: To investigate the effect of DIO on the kinematics and viability of spermatozoa in an albino rat model.
Methods: Sperm suspensions from normal (Control) and diet-induced obese (DIO) Wistar rats were collected and incubated for various times (30, 60, 120 or 180 min at 37 °C). Motility parameters were analyzed with computer-aided sperm analysis (CASA), while viability was assessed by means of a dye exclusion staining technique (eosin/nigrosin).
Results: Results reveal that there was a significant time dependent decrease (P < 0.05) in progressive motility, curvilinear velocity and beat cross-frequency after 60 min, while amplitude of lateral head displacement and sperm viability was significantly reduced (P < 0.05) after 120 min in the DIO group compared to control spermatozoa.
Conclusions: These results provided evidence that obesity is detrimental to sperm parameter in rats possibly through increased testicular temperature as a result of a rise in fat deposition.
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