Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i>
Studies on microorganism response spaceflight date back to 1960. However, nothing conclusive is known concerning the effects of spaceflight on virulence and environmental tolerance of entomopathogenic fungi; thus, this area of research remains open to further exploration. In this study, the entomopa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/8/555 |
| _version_ | 1827617502659608576 |
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| author | Youdan Zhang Xiaochen Zhang Jieming Zhang Shaukat Ali Jianhui Wu |
| author_facet | Youdan Zhang Xiaochen Zhang Jieming Zhang Shaukat Ali Jianhui Wu |
| author_sort | Youdan Zhang |
| collection | DOAJ |
| description | Studies on microorganism response spaceflight date back to 1960. However, nothing conclusive is known concerning the effects of spaceflight on virulence and environmental tolerance of entomopathogenic fungi; thus, this area of research remains open to further exploration. In this study, the entomopathogenic fungus <i>Beauveria bassiana</i> (strain SB010) was exposed to spaceflight (ChangZheng 5 space shuttle during 5 May 2020 to 8 May 2020) as a part of the Key Research and Development Program of Guangdong Province, China, in collaboration with the China Space Program. The study revealed significant differences between the secondary metabolite profiles of the wild isolate (SB010) and the spaceflight-exposed isolate (BHT021, BH030, BHT098) of <i>B. bassiana</i>. Some of the secondary metabolites/toxins, including enniatin A2, brevianamide F, macrosporin, aphidicolin, and diacetoxyscirpenol, were only produced by the spaceflight-exposed isolate (BHT021, BHT030). The study revealed increased insecticidal activities for of crude protein extracts of <i>B. bassiana</i> spaceflight mutants (BHT021 and BH030, respectively) against <i>Megalurothrips usitatus</i> 5 days post application when compared crude protein extracts of the wild isolate (SB010). The data obtained support the idea of using space mutation as a tool for development/screening of fungal strains producing higher quantities of secondary metabolites, ultimately leading to increased toxicity/virulence against the target insect host. |
| first_indexed | 2024-03-09T09:47:55Z |
| format | Article |
| id | doaj.art-8350bcd7639948ac976e798adf166710 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T09:47:55Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-8350bcd7639948ac976e798adf1667102023-12-02T00:23:33ZengMDPI AGToxins2072-66512022-08-0114855510.3390/toxins14080555Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i>Youdan Zhang0Xiaochen Zhang1Jieming Zhang2Shaukat Ali3Jianhui Wu4Key Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaStudies on microorganism response spaceflight date back to 1960. However, nothing conclusive is known concerning the effects of spaceflight on virulence and environmental tolerance of entomopathogenic fungi; thus, this area of research remains open to further exploration. In this study, the entomopathogenic fungus <i>Beauveria bassiana</i> (strain SB010) was exposed to spaceflight (ChangZheng 5 space shuttle during 5 May 2020 to 8 May 2020) as a part of the Key Research and Development Program of Guangdong Province, China, in collaboration with the China Space Program. The study revealed significant differences between the secondary metabolite profiles of the wild isolate (SB010) and the spaceflight-exposed isolate (BHT021, BH030, BHT098) of <i>B. bassiana</i>. Some of the secondary metabolites/toxins, including enniatin A2, brevianamide F, macrosporin, aphidicolin, and diacetoxyscirpenol, were only produced by the spaceflight-exposed isolate (BHT021, BHT030). The study revealed increased insecticidal activities for of crude protein extracts of <i>B. bassiana</i> spaceflight mutants (BHT021 and BH030, respectively) against <i>Megalurothrips usitatus</i> 5 days post application when compared crude protein extracts of the wild isolate (SB010). The data obtained support the idea of using space mutation as a tool for development/screening of fungal strains producing higher quantities of secondary metabolites, ultimately leading to increased toxicity/virulence against the target insect host.https://www.mdpi.com/2072-6651/14/8/555space exposureentomopathogenic fungipathogenicitysecondary metabolites |
| spellingShingle | Youdan Zhang Xiaochen Zhang Jieming Zhang Shaukat Ali Jianhui Wu Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> Toxins space exposure entomopathogenic fungi pathogenicity secondary metabolites |
| title | Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> |
| title_full | Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> |
| title_fullStr | Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> |
| title_full_unstemmed | Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> |
| title_short | Spaceflight Changes the Production and Bioactivity of Secondary Metabolites in <i>Beauveria bassiana</i> |
| title_sort | spaceflight changes the production and bioactivity of secondary metabolites in i beauveria bassiana i |
| topic | space exposure entomopathogenic fungi pathogenicity secondary metabolites |
| url | https://www.mdpi.com/2072-6651/14/8/555 |
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