The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy
Abstract Mangiferin (MAN) was a natural bioactive xanthone with remarkable antioxidative, anti‐inflammatory, and antiapoptotic effects. The purpose of this study to determine the protective influence of MAN on D‐galactose (D‐gal)‐induced aging and cognitive impairment, together with its underlying m...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.306 |
| _version_ | 1797389148986802176 |
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| author | Jing Zhang Yanmei Chen Zhengxuan Wang Wenbing Zhou Hafiz Ullah Jianxin Cao Yaping Liu Guiguang Cheng |
| author_facet | Jing Zhang Yanmei Chen Zhengxuan Wang Wenbing Zhou Hafiz Ullah Jianxin Cao Yaping Liu Guiguang Cheng |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Abstract Mangiferin (MAN) was a natural bioactive xanthone with remarkable antioxidative, anti‐inflammatory, and antiapoptotic effects. The purpose of this study to determine the protective influence of MAN on D‐galactose (D‐gal)‐induced aging and cognitive impairment, together with its underlying molecular mechanism. The network pharmacology molecular docking analysis revealed that MAN has a potential binding with aging‐related oxidative stress and apoptosis proteins. In D‐gal‐induced aging mice, MAN could significantly reduce D‐gal‐induced histopathological changes of liver and brain tissues. Moreover, MAN administration obviously increased activities of antioxidant enzymes (SOD, T‐AOC, GSH‐Px, and CAT) and decreased levels of AChE and MDA in the serum, liver, or brain tissues. Additionally, MAN alleviated learning and memory disorders and improved the exploration ability. Western blotting and immunohistochemical analysis results revealed that MAN could regulate the expressions of PI3K/Akt/Nrf2 signaling pathway‐related proteins and apoptotic‐related proteins (Bax, Bcl‐2, and Caspase‐9) in the liver tissues. Therefore, these findings suggested that MAN can be used as a potent dietary supplement in functional foods for the protection against aging‐related impairments. |
| first_indexed | 2024-03-08T22:51:39Z |
| format | Article |
| id | doaj.art-8357725fa3154cd9aded8f4c0f8d70ce |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-08T22:51:39Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-8357725fa3154cd9aded8f4c0f8d70ce2023-12-16T12:46:30ZengWileyFood Frontiers2643-84292023-12-01441927194510.1002/fft2.306The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategyJing Zhang0Yanmei Chen1Zhengxuan Wang2Wenbing Zhou3Hafiz Ullah4Jianxin Cao5Yaping Liu6Guiguang Cheng7Faculty of Food Science and Engineering Kunming University of Science and Technology Kunming ChinaFaculty of Life Science and Technology Kunming University of Science and Technology Kunming ChinaFaculty of Food Science and Engineering Kunming University of Science and Technology Kunming ChinaYunnan Tobacco Company, Yuxi Branch Yuxi ChinaDepartment of Botany University of Chitral Chitral PakistanFaculty of Food Science and Engineering Kunming University of Science and Technology Kunming ChinaFaculty of Food Science and Engineering Kunming University of Science and Technology Kunming ChinaFaculty of Food Science and Engineering Kunming University of Science and Technology Kunming ChinaAbstract Mangiferin (MAN) was a natural bioactive xanthone with remarkable antioxidative, anti‐inflammatory, and antiapoptotic effects. The purpose of this study to determine the protective influence of MAN on D‐galactose (D‐gal)‐induced aging and cognitive impairment, together with its underlying molecular mechanism. The network pharmacology molecular docking analysis revealed that MAN has a potential binding with aging‐related oxidative stress and apoptosis proteins. In D‐gal‐induced aging mice, MAN could significantly reduce D‐gal‐induced histopathological changes of liver and brain tissues. Moreover, MAN administration obviously increased activities of antioxidant enzymes (SOD, T‐AOC, GSH‐Px, and CAT) and decreased levels of AChE and MDA in the serum, liver, or brain tissues. Additionally, MAN alleviated learning and memory disorders and improved the exploration ability. Western blotting and immunohistochemical analysis results revealed that MAN could regulate the expressions of PI3K/Akt/Nrf2 signaling pathway‐related proteins and apoptotic‐related proteins (Bax, Bcl‐2, and Caspase‐9) in the liver tissues. Therefore, these findings suggested that MAN can be used as a potent dietary supplement in functional foods for the protection against aging‐related impairments.https://doi.org/10.1002/fft2.306agingcognitive impairmentmangiferinnetwork pharmacologyoxidative stresspharmacological mechanism |
| spellingShingle | Jing Zhang Yanmei Chen Zhengxuan Wang Wenbing Zhou Hafiz Ullah Jianxin Cao Yaping Liu Guiguang Cheng The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy Food Frontiers aging cognitive impairment mangiferin network pharmacology oxidative stress pharmacological mechanism |
| title | The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| title_full | The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| title_fullStr | The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| title_full_unstemmed | The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| title_short | The protective effect and mechanism of mangiferin on D‐galactose‐induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| title_sort | protective effect and mechanism of mangiferin on d galactose induced oxidative stress and cognitive impairment in aging mice by an integrated network pharmacology and experimental validation strategy |
| topic | aging cognitive impairment mangiferin network pharmacology oxidative stress pharmacological mechanism |
| url | https://doi.org/10.1002/fft2.306 |
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