Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2
The dysregulated lncRNA play essential roles in glioma development. This study aimed to investigate the role and mechanism of lncRNA potassium voltage-gated channel subfamily Q member 1 opposite strand/ antisense transcript 1 (KCNQ1OT1) in glioma progression. Tumor tissues and adjacent normal sample...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-03-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2020-0012 |
| _version_ | 1819016157499228160 |
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| author | Yin Zhangxing Liao Liqing Mao Sheng Liu Ying Xie Tao Yu Hua Zhao Wenxu |
| author_facet | Yin Zhangxing Liao Liqing Mao Sheng Liu Ying Xie Tao Yu Hua Zhao Wenxu |
| author_sort | Yin Zhangxing |
| collection | DOAJ |
| description | The dysregulated lncRNA play essential roles in glioma development. This study aimed to investigate the role and mechanism of lncRNA potassium voltage-gated channel subfamily Q member 1 opposite strand/ antisense transcript 1 (KCNQ1OT1) in glioma progression. Tumor tissues and adjacent normal samples were collected from 30 glioma patients. The expression levels of lncRNA KCNQ1OT1, microRNA (miR)-338-3p and ribonucleotide reductase M2 (RRM2) were detected by quantitative real-time polymerase chain reaction or western blot analyses. Levels of cell viability, apoptosis, cell migration and invasion in glioma cell lines were determined using cell counting kit-8, flow cytometry with annexin V-FITC and trans-well assays, respectively. The role of KCNQ1OT1 in glioma development in vivo was investigated using a xenograft model. The target association between miR-338-3p and KCNQ1OT1 or RRM2 was validated by luciferase reporter assay. The results found that expression of KCNQ1OT1 was enhanced in glioma tissues and cells, and KCNQ1OT1 knockdown inhibited cell viability, migration and invasion, and xenograft tumor growth, but promoted apoptosis. miR-338-3p was targeted via KCNQ1OT1 and could reverse the effect of KCNQ1OT1 on glioma progression. RRM2 was targeted via miR-338-3p and attenuated the suppressive effect of miR-338-3p on glioma cell viability, migration and invasion. Besides, KCNQ1OT1 overexpression increased RRM2 expression, and this event was weakened via miR-338-3p up-regulation. In conclusion, the present finding suggest that silencing of KCNQ1OT1 can suppress the development and progression of glioma by up-regulating miR-338-3p and down-regulating RRM2. |
| first_indexed | 2024-12-21T02:43:10Z |
| format | Article |
| id | doaj.art-8357abece731445f9f491a149e91bc19 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-21T02:43:10Z |
| publishDate | 2020-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-8357abece731445f9f491a149e91bc192022-12-21T19:18:38ZengDe GruyterOpen Life Sciences2391-54122020-03-0115110812110.1515/biol-2020-0012biol-2020-0012Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2Yin Zhangxing0Liao Liqing1Mao Sheng2Liu Ying3Xie Tao4Yu Hua5Zhao Wenxu6Department of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaDepartment of Neurosurgery, Qianjiang Central Hospital of Hubei Province, No. 22, Zhanghuazhong Road, Qianjiang, 433100, Hubei, ChinaThe dysregulated lncRNA play essential roles in glioma development. This study aimed to investigate the role and mechanism of lncRNA potassium voltage-gated channel subfamily Q member 1 opposite strand/ antisense transcript 1 (KCNQ1OT1) in glioma progression. Tumor tissues and adjacent normal samples were collected from 30 glioma patients. The expression levels of lncRNA KCNQ1OT1, microRNA (miR)-338-3p and ribonucleotide reductase M2 (RRM2) were detected by quantitative real-time polymerase chain reaction or western blot analyses. Levels of cell viability, apoptosis, cell migration and invasion in glioma cell lines were determined using cell counting kit-8, flow cytometry with annexin V-FITC and trans-well assays, respectively. The role of KCNQ1OT1 in glioma development in vivo was investigated using a xenograft model. The target association between miR-338-3p and KCNQ1OT1 or RRM2 was validated by luciferase reporter assay. The results found that expression of KCNQ1OT1 was enhanced in glioma tissues and cells, and KCNQ1OT1 knockdown inhibited cell viability, migration and invasion, and xenograft tumor growth, but promoted apoptosis. miR-338-3p was targeted via KCNQ1OT1 and could reverse the effect of KCNQ1OT1 on glioma progression. RRM2 was targeted via miR-338-3p and attenuated the suppressive effect of miR-338-3p on glioma cell viability, migration and invasion. Besides, KCNQ1OT1 overexpression increased RRM2 expression, and this event was weakened via miR-338-3p up-regulation. In conclusion, the present finding suggest that silencing of KCNQ1OT1 can suppress the development and progression of glioma by up-regulating miR-338-3p and down-regulating RRM2.https://doi.org/10.1515/biol-2020-0012gliomakcnq1ot1mir-338-3prrm2 |
| spellingShingle | Yin Zhangxing Liao Liqing Mao Sheng Liu Ying Xie Tao Yu Hua Zhao Wenxu Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 Open Life Sciences glioma kcnq1ot1 mir-338-3p rrm2 |
| title | Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 |
| title_full | Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 |
| title_fullStr | Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 |
| title_full_unstemmed | Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 |
| title_short | Knockdown of lncRNA KCNQ1OT1 inhibits glioma progression by regulating miR-338-3p/RRM2 |
| title_sort | knockdown of lncrna kcnq1ot1 inhibits glioma progression by regulating mir 338 3p rrm2 |
| topic | glioma kcnq1ot1 mir-338-3p rrm2 |
| url | https://doi.org/10.1515/biol-2020-0012 |
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