Ecogenotoxicity testing of aquatic environment by comet assay in plants
One of the goals of environmental monitoring is the detection of potentially hazardous compounds in water. We have set up a standard method to apply the Comet assay in aquatic plants that could be of great interest to evaluate cytotoxicity, genotoxicity and oxidative stress on the same species regar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00051/full |
| _version_ | 1819088975973842944 |
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| author | Anita Mukherjee |
| author_facet | Anita Mukherjee |
| author_sort | Anita Mukherjee |
| collection | DOAJ |
| description | One of the goals of environmental monitoring is the detection of potentially hazardous compounds in water. We have set up a standard method to apply the Comet assay in aquatic plants that could be of great interest to evaluate cytotoxicity, genotoxicity and oxidative stress on the same species regarded as most sensitive to environmental pollutants.
The aim of the present study was to set up of standardized procedure to evaluate genotoxicity in aquatic plants- Ceratophyllum demersum one that is submerged free floating and the other is Lemna minor - a fresh water floating plant by Comet assay. Electrophoresis and unwinding times were adapted to obtain minimum DNA migration evaluated as tail intensity % or tail moment in the control group and, at the same time maximum sensitivity for DNA damage with known genotoxicants. We further investigated the cytotoxicity and oxidative stress induced in the same species. Based on the repeatability of results obtained we suggest that Ceratophyllum, Lemna can serve as model species and Comet assay could be adopted to monitor the eco-genotoxicity of water pollutants. |
| first_indexed | 2024-12-21T22:00:35Z |
| format | Article |
| id | doaj.art-83581f3e0437464cac17cdff45d09770 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T22:00:35Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-83581f3e0437464cac17cdff45d097702022-12-21T18:48:50ZengFrontiers Media S.A.Frontiers in Genetics1664-80212015-05-01610.3389/conf.fgene.2015.01.00051152172Ecogenotoxicity testing of aquatic environment by comet assay in plantsAnita Mukherjee0University of CalcuttaOne of the goals of environmental monitoring is the detection of potentially hazardous compounds in water. We have set up a standard method to apply the Comet assay in aquatic plants that could be of great interest to evaluate cytotoxicity, genotoxicity and oxidative stress on the same species regarded as most sensitive to environmental pollutants. The aim of the present study was to set up of standardized procedure to evaluate genotoxicity in aquatic plants- Ceratophyllum demersum one that is submerged free floating and the other is Lemna minor - a fresh water floating plant by Comet assay. Electrophoresis and unwinding times were adapted to obtain minimum DNA migration evaluated as tail intensity % or tail moment in the control group and, at the same time maximum sensitivity for DNA damage with known genotoxicants. We further investigated the cytotoxicity and oxidative stress induced in the same species. Based on the repeatability of results obtained we suggest that Ceratophyllum, Lemna can serve as model species and Comet assay could be adopted to monitor the eco-genotoxicity of water pollutants.http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00051/fullComet AssayAntioxidant EnzymesLemnaCeratophyllumFly Ash Leachate |
| spellingShingle | Anita Mukherjee Ecogenotoxicity testing of aquatic environment by comet assay in plants Frontiers in Genetics Comet Assay Antioxidant Enzymes Lemna Ceratophyllum Fly Ash Leachate |
| title | Ecogenotoxicity testing of aquatic environment by comet assay in plants |
| title_full | Ecogenotoxicity testing of aquatic environment by comet assay in plants |
| title_fullStr | Ecogenotoxicity testing of aquatic environment by comet assay in plants |
| title_full_unstemmed | Ecogenotoxicity testing of aquatic environment by comet assay in plants |
| title_short | Ecogenotoxicity testing of aquatic environment by comet assay in plants |
| title_sort | ecogenotoxicity testing of aquatic environment by comet assay in plants |
| topic | Comet Assay Antioxidant Enzymes Lemna Ceratophyllum Fly Ash Leachate |
| url | http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00051/full |
| work_keys_str_mv | AT anitamukherjee ecogenotoxicitytestingofaquaticenvironmentbycometassayinplants |