M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations
Background: Macrophages (Mф) can be M1/M2 polarized by Th1/2 signals, respectively. M2-like Mф are thought to be important in asthma pathogenesis, and M1-like in anti-infective immunity, however their roles in virus-induced asthma exacerbations are unknown. Our objectives were (i) to assess polarise...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-04-01
|
| Series: | EBioMedicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396420301092 |
| _version_ | 1818062146939912192 |
|---|---|
| author | Alexandra Nikonova Musa Khaitov David J. Jackson Stephanie Traub Maria-Belen Trujillo-Torralbo Dmitriy A. Kudlay Anton S. Dvornikov Ajerico del-Rosario Rudolf Valenta Luminita A. Stanciu Rahim Khaitov Sebastian L. Johnston |
| author_facet | Alexandra Nikonova Musa Khaitov David J. Jackson Stephanie Traub Maria-Belen Trujillo-Torralbo Dmitriy A. Kudlay Anton S. Dvornikov Ajerico del-Rosario Rudolf Valenta Luminita A. Stanciu Rahim Khaitov Sebastian L. Johnston |
| author_sort | Alexandra Nikonova |
| collection | DOAJ |
| description | Background: Macrophages (Mф) can be M1/M2 polarized by Th1/2 signals, respectively. M2-like Mф are thought to be important in asthma pathogenesis, and M1-like in anti-infective immunity, however their roles in virus-induced asthma exacerbations are unknown. Our objectives were (i) to assess polarised Mф phenotype responses to rhinovirus (RV) infection in vitro and (ii) to assess Mф phenotypes in healthy subjects and people with asthma before and during experimental RV infection in vivo. Methods: We investigated characteristics of polarized/unpolarized human monocyte-derived Mф (MDM, from 3–6 independent donors) in vitro and evaluated frequencies of M1/M2-like bronchoalveolar lavage (BAL) Mф in experimental RV-induced asthma exacerbation in 7 healthy controls and 17 (at baseline) and 18 (at day 4 post infection) people with asthma. Findings: We observed in vitro: M1-like but not M2-like or unpolarized MDM are potent producers of type I and III interferons in response to RV infection (P<0.0001), and M1-like are more resistant to RV infection (P<0.05); compared to M1-like, M2-like MDM constitutively produced higher levels of CCL22/MDC (P = 0.007) and CCL17/TARC (P<0.0001); RV-infected M1-like MDM were characterized as CD14+CD80+CD197+ (P = 0.002 vs M2-like, P<0.0001 vs unpolarized MDM). In vivo we found reduced percentages of M1-like CD14+CD80+CD197+ BAL Mф in asthma during experimental RV16 infection compared to baseline (P = 0.024). Interpretation: Human M1-like BAL Mф are likely important contributors to anti-viral immunity and their numbers are reduced in patients with allergic asthma during RV-induced asthma exacerbations. This mechanism may be one explanation why RV-triggered clinical and pathologic outcomes are more severe in allergic patients than in healthy subjects. Funding: ERC FP7 Advanced grant 233015, MRC Centre Grant G1000758, Asthma UK grant 08–048, NIHR Biomedical Research Centre funding scheme, NIHR BRC Centre grant P26095, the Predicta FP7 Collaborative Project grant 260895, RSF grant 19-15-00272, Megagrant No 14.W03.31.0024. Key words: Macrophage, Polarization, Rhinovirus, Asthma, Exacerbation |
| first_indexed | 2024-12-10T13:59:35Z |
| format | Article |
| id | doaj.art-83599ee0d791431fa8664baecb15c64e |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-10T13:59:35Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-83599ee0d791431fa8664baecb15c64e2022-12-22T01:45:50ZengElsevierEBioMedicine2352-39642020-04-0154M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbationsAlexandra Nikonova0Musa Khaitov1David J. Jackson2Stephanie Traub3Maria-Belen Trujillo-Torralbo4Dmitriy A. Kudlay5Anton S. Dvornikov6Ajerico del-Rosario7Rudolf Valenta8Luminita A. Stanciu9Rahim Khaitov10Sebastian L. Johnston11National Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Norfolk Place, London W2 1PG, United Kingdom; NRC Institute of Immunology FMBA, Kashirskoe shosse 24, 115478 Moscow, Russian Federation; Mechnikov Research Institute for Vaccines and Sera, M. Kazenny per., 5A, 105064 Moscow, Russian FederationNRC Institute of Immunology FMBA, Kashirskoe shosse 24, 115478 Moscow, Russian FederationNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; Imperial College Healthcare NHS Trust, Norfolk Place, London W2 1PG, United Kingdom; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Norfolk Place, London W2 1PG, United KingdomNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Norfolk Place, London W2 1PG, United KingdomNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; Imperial College Healthcare NHS Trust, Norfolk Place, London W2 1PG, United KingdomNRC Institute of Immunology FMBA, Kashirskoe shosse 24, 115478 Moscow, Russian FederationPirogov Russian National Research Medical University, Ostrovitianov str. 1, 117513 Moscow, Russian FederationNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; Imperial College Healthcare NHS Trust, Norfolk Place, London W2 1PG, United KingdomNRC Institute of Immunology FMBA, Kashirskoe shosse 24, 115478 Moscow, Russian Federation; Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, AustriaNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Norfolk Place, London W2 1PG, United KingdomNRC Institute of Immunology FMBA, Kashirskoe shosse 24, 115478 Moscow, Russian FederationNational Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom; Imperial College Healthcare NHS Trust, Norfolk Place, London W2 1PG, United Kingdom; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Norfolk Place, London W2 1PG, United Kingdom; Corresponding author at: National Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom.Background: Macrophages (Mф) can be M1/M2 polarized by Th1/2 signals, respectively. M2-like Mф are thought to be important in asthma pathogenesis, and M1-like in anti-infective immunity, however their roles in virus-induced asthma exacerbations are unknown. Our objectives were (i) to assess polarised Mф phenotype responses to rhinovirus (RV) infection in vitro and (ii) to assess Mф phenotypes in healthy subjects and people with asthma before and during experimental RV infection in vivo. Methods: We investigated characteristics of polarized/unpolarized human monocyte-derived Mф (MDM, from 3–6 independent donors) in vitro and evaluated frequencies of M1/M2-like bronchoalveolar lavage (BAL) Mф in experimental RV-induced asthma exacerbation in 7 healthy controls and 17 (at baseline) and 18 (at day 4 post infection) people with asthma. Findings: We observed in vitro: M1-like but not M2-like or unpolarized MDM are potent producers of type I and III interferons in response to RV infection (P<0.0001), and M1-like are more resistant to RV infection (P<0.05); compared to M1-like, M2-like MDM constitutively produced higher levels of CCL22/MDC (P = 0.007) and CCL17/TARC (P<0.0001); RV-infected M1-like MDM were characterized as CD14+CD80+CD197+ (P = 0.002 vs M2-like, P<0.0001 vs unpolarized MDM). In vivo we found reduced percentages of M1-like CD14+CD80+CD197+ BAL Mф in asthma during experimental RV16 infection compared to baseline (P = 0.024). Interpretation: Human M1-like BAL Mф are likely important contributors to anti-viral immunity and their numbers are reduced in patients with allergic asthma during RV-induced asthma exacerbations. This mechanism may be one explanation why RV-triggered clinical and pathologic outcomes are more severe in allergic patients than in healthy subjects. Funding: ERC FP7 Advanced grant 233015, MRC Centre Grant G1000758, Asthma UK grant 08–048, NIHR Biomedical Research Centre funding scheme, NIHR BRC Centre grant P26095, the Predicta FP7 Collaborative Project grant 260895, RSF grant 19-15-00272, Megagrant No 14.W03.31.0024. Key words: Macrophage, Polarization, Rhinovirus, Asthma, Exacerbationhttp://www.sciencedirect.com/science/article/pii/S2352396420301092 |
| spellingShingle | Alexandra Nikonova Musa Khaitov David J. Jackson Stephanie Traub Maria-Belen Trujillo-Torralbo Dmitriy A. Kudlay Anton S. Dvornikov Ajerico del-Rosario Rudolf Valenta Luminita A. Stanciu Rahim Khaitov Sebastian L. Johnston M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations EBioMedicine |
| title | M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations |
| title_full | M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations |
| title_fullStr | M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations |
| title_full_unstemmed | M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations |
| title_short | M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations |
| title_sort | m1 like macrophages are potent producers of anti viral interferons and m1 associated marker positive lung macrophages are decreased during rhinovirus induced asthma exacerbations |
| url | http://www.sciencedirect.com/science/article/pii/S2352396420301092 |
| work_keys_str_mv | AT alexandranikonova m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT musakhaitov m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT davidjjackson m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT stephanietraub m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT mariabelentrujillotorralbo m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT dmitriyakudlay m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT antonsdvornikov m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT ajericodelrosario m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT rudolfvalenta m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT luminitaastanciu m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT rahimkhaitov m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations AT sebastianljohnston m1likemacrophagesarepotentproducersofantiviralinterferonsandm1associatedmarkerpositivelungmacrophagesaredecreasedduringrhinovirusinducedasthmaexacerbations |