Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids
ABSTRACT: The effects of grass silage and red clover silage on milk fatty acid (FA) composition are extensively studied, but little is known of their effects on minor lipid constituents of milk fat globule membrane. We investigated the effects of forage:concentrate (FC) ratio in grass silage-based d...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223007208 |
| _version_ | 1797291839489835008 |
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| author | Mari J. Jaakamo Tytti J. Luukkonen Piia K. Kairenius Ali R. Bayat Seppo A. Ahvenjärvi Johanna H. Vilkki Heidi M. Leskinen |
| author_facet | Mari J. Jaakamo Tytti J. Luukkonen Piia K. Kairenius Ali R. Bayat Seppo A. Ahvenjärvi Johanna H. Vilkki Heidi M. Leskinen |
| author_sort | Mari J. Jaakamo |
| collection | DOAJ |
| description | ABSTRACT: The effects of grass silage and red clover silage on milk fatty acid (FA) composition are extensively studied, but little is known of their effects on minor lipid constituents of milk fat globule membrane. We investigated the effects of forage:concentrate (FC) ratio in grass silage-based diets and forage type (grass silage vs. red clover silage) on selected molecular species of milk phospholipids (PL) and the FA composition of PL. Ten multiparous Nordic Red cows were offered following dietary treatments: grass silage-based diets containing 70:30 (HG) or 30:70 (LG) FC ratio or a red clover silage-based diet (RC) comprising 50:50 FC ratio on a dry matter basis. The most abundant molecular species within the phosphatidylcholines was 16:0–18:1 phosphatidylcholine that was increased by 18% in HG compared with LG milk. Dietary treatments did not affect the relative proportion of 18:1–18:1+18:0–18:2 phosphatidylethanolamine that was the most prevalent species (ca. 44%–45%) in that class. We identified the d18:1–22:0 sphingomyelin as the most abundant sphingomyelin species that tended to increase in HG milk compared with LG. The FC ratio did not affect the relative proportions of saturated FA nor monounsaturated FA in PL, but the proportion of cis-9 18:1 was elevated in HG versus LG milk, whereas the proportion of 18:2n-6 was 50% higher in LG versus HG milk. The RC diet increased monounsaturated FA and 18:3n-3 levels in PL compared with grass silage-based diets and decreased the relative proportion of saturated FA. However, the RC diet did not affect the relative proportion of polyunsaturated FA in PL, although red clover silage typically increases the proportion of polyunsaturated FA in milk fat. This study provides valuable knowledge of the minor lipid components in milk on species level in relation to common feeding strategies in high-forage systems. |
| first_indexed | 2024-03-07T19:43:33Z |
| format | Article |
| id | doaj.art-8359e0c987f14a32a39528076da2a020 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-07T19:43:33Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-8359e0c987f14a32a39528076da2a0202024-02-29T05:17:01ZengElsevierJournal of Dairy Science0022-03022024-03-01107314501459Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipidsMari J. Jaakamo0Tytti J. Luukkonen1Piia K. Kairenius2Ali R. Bayat3Seppo A. Ahvenjärvi4Johanna H. Vilkki5Heidi M. Leskinen6Animal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, Finland; Corresponding authorsAnimal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, FinlandAnimal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, FinlandAnimal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, FinlandAnimal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, FinlandGenomics and Breeding, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, FinlandAnimal Nutrition, Production Systems, Natural Resources Institute Finland, FI-31600 Jokioinen, Finland; Corresponding authorsABSTRACT: The effects of grass silage and red clover silage on milk fatty acid (FA) composition are extensively studied, but little is known of their effects on minor lipid constituents of milk fat globule membrane. We investigated the effects of forage:concentrate (FC) ratio in grass silage-based diets and forage type (grass silage vs. red clover silage) on selected molecular species of milk phospholipids (PL) and the FA composition of PL. Ten multiparous Nordic Red cows were offered following dietary treatments: grass silage-based diets containing 70:30 (HG) or 30:70 (LG) FC ratio or a red clover silage-based diet (RC) comprising 50:50 FC ratio on a dry matter basis. The most abundant molecular species within the phosphatidylcholines was 16:0–18:1 phosphatidylcholine that was increased by 18% in HG compared with LG milk. Dietary treatments did not affect the relative proportion of 18:1–18:1+18:0–18:2 phosphatidylethanolamine that was the most prevalent species (ca. 44%–45%) in that class. We identified the d18:1–22:0 sphingomyelin as the most abundant sphingomyelin species that tended to increase in HG milk compared with LG. The FC ratio did not affect the relative proportions of saturated FA nor monounsaturated FA in PL, but the proportion of cis-9 18:1 was elevated in HG versus LG milk, whereas the proportion of 18:2n-6 was 50% higher in LG versus HG milk. The RC diet increased monounsaturated FA and 18:3n-3 levels in PL compared with grass silage-based diets and decreased the relative proportion of saturated FA. However, the RC diet did not affect the relative proportion of polyunsaturated FA in PL, although red clover silage typically increases the proportion of polyunsaturated FA in milk fat. This study provides valuable knowledge of the minor lipid components in milk on species level in relation to common feeding strategies in high-forage systems.http://www.sciencedirect.com/science/article/pii/S0022030223007208grass silagered clover silagephospholipidpolar lipidfatty acid composition |
| spellingShingle | Mari J. Jaakamo Tytti J. Luukkonen Piia K. Kairenius Ali R. Bayat Seppo A. Ahvenjärvi Johanna H. Vilkki Heidi M. Leskinen Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids Journal of Dairy Science grass silage red clover silage phospholipid polar lipid fatty acid composition |
| title | Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| title_full | Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| title_fullStr | Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| title_full_unstemmed | Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| title_short | Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| title_sort | effects of dietary forage to concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids |
| topic | grass silage red clover silage phospholipid polar lipid fatty acid composition |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223007208 |
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